Citric acid monohydrate, acetyl-β-methylcholine chloride, salbutamol sulfate, atropine, cinnamaldehyde, codeine phosphate, chlorpheniramine maleate (Sigma-Aldrich, Poole, UK), and levodropropizine, (Eurodrug, The Hague, Netherlands), were all dissolved in sterile saline (0.9%; Baxter Healthcare Ltd., Thetford, UK). Tiotropium bromide (Chiesi Farmaceutici, Parma, Italy) was prepared as a stock solution of 6 mM and diluted with sterile saline. All drugs were prepared fresh on the day of use. Roflumilast (CAS162401-32-3; Kemprotec, Carnforth, UK) is poorly soluble in saline and was therefore prepared in neat solutol (2%, Kolliphor HS15, 42966; Sigma-Aldrich) and diluted to the final concentration on the day of use. Capsaicin (Sigma-Aldrich) was prepared as a stock solution of 25 mg/ml (8:1:1 saline:ethanol:Tween 20; Fisher Scientific, Loughborough, UK). HC-030031 (2-(1,3-dimethyl-2,6-dioxo-1,2,3,6-tetrahydro-7H-purin-7-yl)-N-(4-isopropylphenyl)acetamide; Chiesi Farmaceutici) was prepared as a stock solution of 300 mg/ml in sterile saline and 1% solutol.
Chlorpheniramine maleate
Manufactured by Merck Group
Sourced in United Kingdom, United States, Germany
Chlorpheniramine maleate is a synthetic antihistamine compound used in various pharmaceutical and laboratory applications. It functions as a histamine H1 receptor antagonist, which means it blocks the effects of the histamine chemical in the body. This compound is commonly used in the production of various medications and as a research tool in scientific experiments.
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Lab products found in correlation
Kolliphor hs 15, by Merck Group (1 mentions)
Capsaicin, by Merck Group (1 mentions)
Cinnamaldehyde, by Merck Group (1 mentions)
Codeine phosphate, by Merck Group (1 mentions)
Chloroquine cq, by Merck Group (1 mentions)
Histamine, by Merck Group (1 mentions)
Resiniferatoxin rtx, by Merck Group (1 mentions)
Compound 48 80, by Merck Group (1 mentions)
Amiloride hydrochloride, by Merck Group (1 mentions)
Naloxone hydrochloride, by Merck Group (1 mentions)
Agmatine sulfate salt, by Merck Group (1 mentions)
Capsazepine, by Bio-Techne (1 mentions)
Apetx2, by Bio-Techne (1 mentions)
Mc903, by R&D Systems (1 mentions)
Triethylamine tea, by Merck Group (1 mentions)
Sodium phosphate, by Merck Group (1 mentions)
Chlorhexidine digluconate, by Merck Group (1 mentions)
Whatman, by Cytiva (1 mentions)
Isobutylmethylxanthine, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
4 protocols using chlorpheniramine maleate
1
Preparation of Pharmaceutical Compounds
2
Reagents for Pain and Inflammation Study
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Agmatine sulfate salt (Cat#a7127), histamine (Cat#y0001779), compound 48/80 (Cat#C2313), resiniferatoxin (RTX, Cat#R8756), chlorpheniramine maleate (Cat#C3025), chloroquine (CQ, Cat#C6628), naloxone hydrochloride (Cat#N3136), amiloride hydrochloride (Cat#1019701) were all obtained from Sigma-Aldrich (St. Louis, MO, United States). MC903 (Cat# 2700/10) was purchased from R&D systems (Minneapolis, MN, United States). Morphine hydrochloride was obtained from China Northeast Pharmaceutical Group Shenyang No. 1 Pharmaceutical Co., Ltd (Shenyang City, China). naloxone hydrochloride HC-030031 (Cat#2896), capsazepine (CPZ, Cat#0464) and APETx2 (Cat#4804) were purchased from Tocris (Minneapolis, MN, United States). ZnCl2 was obtained from National Pharmaceutical Group pharmaceutical Co., Ltd (Beijing, China). RTX and HC-030031 were freshly dissolved in 10% DMSO. Other reagents were dissolved in saline. Information relating to timing and doses are indicated in the results section or figure legends.
3
Quantification of Chlorhexidine and Cytokines in GCF
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Chlorhexidine digluconate and chlorpheniramine maleate (internal standard, IS) were purchased from Sigma Aldrich (Germany) and Supriya Lifescience Ltd. (India), respectively. HPLC grade methanol and acetonitrile (ACN) were supplied by Carlo Erba (Italy). Sodium phosphate, triethylamine (TEA) and phosphoric acid (p.a grade) were also purchased from Sigma Aldrich (Germany). Throughout the entire chromatographic analysis, HPLC water was used. Whatman 3MM chromatography paper strips, 2 × 5 mm (Whatman Lab sales Ltd., UK) were used for GCF collection. Chlorhexamed 1% gel (0.5 g Chlorhexidine digluconate/50 g gel) was purchased from GlaxoSmithCline, GmbH, Buehl, Germany while ATRIDOX 10% gel (45 mg doxycycline hyclate/0.5 g gel) was supplied by TOLMAR Inc. Fort Collins (USA). Protease inhibitor cocktail for cytokine determination was purchased from Sigma Aldrich (Germany). The assay kits for ALP, LDH and AST activity determination were purchased from Biosystems (Spain). The concentration levels of IL-1β and TNF-α in GCF samples were analyzed using commercial ELISA kits (Booster Immunoleader, Fremont, CA).
4
Immune Cell Signaling Modulation
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Cell culture medium, antibiotics, isobutylmethyl xanthine (IBMX), cAMP, prostaglandin E2 (PGE2), 2,3-trifluormetilfenilhistamine dimaleate (histamine H 1 receptor agonist), bovine serum albumin (BSA), phorbol 12-myristate 13-acetate (PMA), lipopolysaccharides from Escherichia Coli (LPS), cimetidine, ranitidine hydrochloride, famotidine, cetirizine dihydrocloride, chlorpheniramine maleate and diphenhydramine hydrochloride were obtained from Sigma Chemical Company (St.
Louis, MO). Amthamine dihydrobromide, mepyramine maleate, triprolidine hydrochloride, tiotidine and dynasore were acquired from Tocris Cookson Inc.
(Ballwin, MO). [ 3 H]cAMP, [ 3 H]mepyramine and [ 3 H]tiotidine, were purchased from Perkin Elmer Life Sciences (Boston, MA). Fetal bovine serum (FBS) was purchased from Natocor (Argentina). Other chemicals used were of analytical grade and obtained from standard sources.
Louis, MO). Amthamine dihydrobromide, mepyramine maleate, triprolidine hydrochloride, tiotidine and dynasore were acquired from Tocris Cookson Inc.
(Ballwin, MO). [ 3 H]cAMP, [ 3 H]mepyramine and [ 3 H]tiotidine, were purchased from Perkin Elmer Life Sciences (Boston, MA). Fetal bovine serum (FBS) was purchased from Natocor (Argentina). Other chemicals used were of analytical grade and obtained from standard sources.
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