A 75‐g oral glucose tolerance test was carried out according to the World Health Organization/American Diabetes Association 2007 criteria. The blood samples were drawn from the antecubital vein. Plasma glucose and insulin concentrations were analyzed using the glucose‐hexokinase method and a radioimmunoassay kit (Beijing North Institute, Beijing, China), respectively. Serum glycosylated hemoglobin (HbAlc%) was measured by affinity chromatography. Serum total cholesterol, low‐density lipoprotein (LDL‐c), high‐density lipoprotein and triglyceride concentrations were determined by using a Cobas Fara robot (Roche, Basel, Switzerland). All the assays were carried out at the Department of Cardiovascular Rehabilitation, Xiangya Hospital of Central South University in China.
Cobas fara robot
Manufactured by Roche
Sourced in Switzerland, France
The Cobas Fara robot is an automated clinical chemistry analyzer designed for high-throughput laboratory testing. It is capable of performing a wide range of clinical chemistry assays, including tests for enzymes, substrates, and other analytes. The Cobas Fara robot is designed to provide efficient and reliable laboratory results.
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Lab products found in correlation
2 protocols using cobas fara robot
1
Oral Glucose Tolerance Test in China
2
Biomarker Measurement Techniques
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IL-6, TNF-α, and insulin were measured using commercially available ELISA kits (IL-6: #HS600B, R&D Systems, Minneapolis, MN, USA; TNF-α: #HSTA00C, R&D Systems; and insulin: #K6219, DakoCytomation, St. Charles, MO, USA). Detection limits were 0.1 pg/mL for IL-6, 0.3 pg/mL for TNF-α, and 10 pmol/L for insulin. Mean intra- and interassay coefficients of variance (CV) were 4% and 6% for insulin, 3% and 20% for TNF-α, and 6% and 20% for IL-6. Free fatty acids (FFA) were measured as previously described [14 (link)]. Glucose measurements were made with an ABX Hexokinase Assay (#A11A01667, ABX Diagnostics, Montpellier, France) on a Cobas Fara robot (Roche) [15 (link)]. Leukocytes were counted using the Sysmex XE-2100 (Sysmex Corporation, Kobe, Japan) equipment at the Department of Clinical Biochemistry 3011 at Rigshospitalet, Copenhagen.
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