Epigallocatechin 3 gallate

Epigallocatechin-3-gallate (EGCG, 95%) was purchased from Dalian Meilun Biotech Co. Ltd (Dalian, China). Zn(NO₃)₂·6H₂O (99.0%), CaCl₂ and Na₂CO₃ were purchased from Tianjin Xindayu Chemical Co. Ltd (Tianjin, China). Hydrogen peroxide (H₂O₂, 30 wt%), poly (sodium. 4-styrenesulfonate) (PSS, MW ca. 70 000) were purchased from Tianjin Guangfu Fine Chemical Research Institute (Tianjin, China). 3-[4,5-dimethylthialzol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich. PBS, anhydrous dimethyl sulfoxide (DMSO, 99.9% purity), RPMI 1640 medium (Hyclone), DMEM-F12, endothelial cell growth medium, fetal bovine serum (FBS), Tween 20, LPS, PMA were purchased from Solarbio Science &Technology Co. Ltd (Beijing, China). 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH free radical, product number: D4313) was purchased from Tokyo Chemical Industry Co. LTD. Transwell BD Matrigel was purchased from Corning Incorporated (New York, USA). Anti-mouse enzyme-linked immunosorbent assay (ELISA) kits, TNF-α, and IL-6 were purchased from eBioscience (San Diego, CA, USA). VEGF, CD31and HIF-1α antibody were obtained from Abcam (Cambridge, UK). PCNA was obtained from Santa Cruz Biotechnology Inc (CA, USA). All chemicals were used without further purification.

Hyaluronic acid (HA, 97%) (MW ≈ 9000 Da) was purchased from Shandong Freda Biopharmaceutical Co., Ltd. Cysteamine dihydrochloride (>97%), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, 97%), and glutathione (GSH, 98%) were purchased from Aladdin (Shanghai, China). Iron chloride hexahydrate (FeCl₃·6H₂O, 99%) was purchased from Sigma-Aldrich. Epigallocatechin-3-gallate (EGCG, 95%) and Doxorubicin (DOX, >98%) were purchased from Meilun Biotechnology Co., Ltd. Annexin V-fluorescein isothiocyanate (FITC)/7-aminoactinomycin D (7AAD), 4′,6-diamidino-2-phenylindole (DAPI), and lysosomal probes were purchased from Beyotime Biotechnology (Shanghai). Antibodies against CD44, CD31, Ki67, β-actin, and caspase 3 were purchased from Abcam (Massachusetts, USA).