Hucytosnp 12 chip

Manufactured by Illumina

The HuCytoSNP-12 chip is a DNA microarray designed by Illumina for genetic analysis. It is used to detect and genotype a large number of single nucleotide polymorphisms (SNPs) across the human genome. The chip contains probes for over 300,000 genetic markers, allowing for comprehensive genome-wide genotyping.

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Lab products found in correlation

Karyostudio v1, by Illumina (2 mentions) Dneasy blood and tissue kit, by Qiagen (2 mentions)

2 protocols using hucytosnp 12 chip

hiPSC SNP Karyotyping Protocol

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Each hiPSC line from p12 was detached from the culture play using trypsin for 10 min and centrifuged at 300g for 3 min. hiPSC pellets were resuspended in 200 μl of PBS and DNA was isolated using the DNeasy blood and tissue kit (Qiagen, Valencia, CA). DNA was submitted to the Stanford Functional Genomics Facility for SNP karyotyping using the HuCytoSNP-12 chip (Illumina). CNV and SNP visualization was performed using KaryoStudio v1.4 (Illumina) (Supplementary Fig. 3).

Churko J.M., Lee J., Ameen M., Gu M., Venkatasubramanian M., Diecke S., Sallam K., Im H., Wang G., Gold J.D., Salomonis N., Snyder M.P, & Wu J.C. (2017). Transcriptomic and epigenomic differences in human induced pluripotent stem cells generated from six reprogramming methods. Nature biomedical engineering, 1(10), 826-837.

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iPSC Karyotyping and CNV Analysis

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BrS lines were trypsinized for 5 min and centrifuged at 300 g for 3 min. iPSC pellets were resuspended in 200 μl of PBS and DNA was isolated using the DNeasy blood and tissue kit (Qiagen, Valencia, CA). DNA was submitted to the Stanford Functional Genomics Facility for SNP karyotyping using the HuCytoSNP-12 chip (Illumina). CNV and SNP visualization was performed using KaryoStudio v1.4 (Illumina).

Liang P., Sallam K., Wu H., Li Y., Itzhaki I., Garg P., Zhang Y., Vermglinchan V., Lan F., Gu M., Gong T., Zhuge Y., He C., Ebert A.D., Sanchez-Freire V., Churko J., Hu S., Sharma A., Lam C.K., Scheinman M.M., Bers D.M, & Wu J.C. (2016). Patient-specific and genome-edited induced pluripotent stem cell-derived cardiomyocytes elucidate single cell phenotype of Brugada Syndrome. Journal of the American College of Cardiology, 68(19), 2086-2096.

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