The human TNBC cell line MDA-MB-231 and human normal breast epithelial cell line MCF-10a were procured from ATCC, while the TNBC cell line CAL-51 was obtained from DSMZ. MDA-MB-231 and CAL-51 cells were cultured in DMEM supplemented with 1% P/S and 10% fetal bovine serum (FBS). MCF-10a cells were cultured in DMEM supplemented with 10% FBS, 20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 10 μg/mL insulin, 1% NEAA, and 1% P/S. The culture conditions were maintained at a constant temperature of 37 °C in a humidified environment containing 5% CO2.
Cal 51
Manufactured by Leibniz Institute DSMZ
Sourced in Germany, Australia
The CAL-51 is a cell line derived from a human lung cancer. It is a well-characterized, immortalized cell line commonly used in cancer research and drug development.
Automatically generated - may contain errors
Lab products found in correlation
Mda mb 231, by American Type Culture Collection (10 mentions)
Mda mb 157, by American Type Culture Collection (6 mentions)
Mda mb 468, by American Type Culture Collection (5 mentions)
Mcf 7, by American Type Culture Collection (4 mentions)
Mda mb 436, by American Type Culture Collection (4 mentions)
Bt549, by American Type Culture Collection (4 mentions)
Mda mb 453, by American Type Culture Collection (4 mentions)
Bt 20, by American Type Culture Collection (4 mentions)
Hs578t, by American Type Culture Collection (4 mentions)
Hcc1806, by American Type Culture Collection (4 mentions)
Cal 148, by Leibniz Institute DSMZ (4 mentions)
Insulin, by Merck Group (3 mentions)
Hcc1954, by American Type Culture Collection (3 mentions)
Hcc38, by American Type Culture Collection (3 mentions)
Cal 85 1, by Leibniz Institute DSMZ (3 mentions)
Hcc1937, by American Type Culture Collection (3 mentions)
Mcf 10a, by American Type Culture Collection (2 mentions)
Efm 192a, by Leibniz Institute DSMZ (2 mentions)
Hydrocortisone, by Merck Group (2 mentions)
Mfm 223, by Leibniz Institute DSMZ (2 mentions)
24 protocols using cal 51
1
Culture of TNBC and Normal Breast Cell Lines
2
Cell Line Cultures and Inhibitors
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MCF-7 and MDA-MB231 cell lines were from American Type Culture Collection (ATCC, Rockville, USA), and Cal51 from DSMZ (Braunschweig, Germany). The ER-Src MCF-10A cell line was a generous gift of Dr Kevin Struhl (Harvard Medical School, Boston, USA). All cell lines were cultured following supplier's recommendations. The ER-Src MCF-10A cells contain an integrated fusion of the v-Src oncoprotein and the ligand-binding domain of estrogen receptor and are induced to rapidly transform when grown with 1 μM 4OH-TAM (Sigma-Aldrich, Saint-Quentin Yvelines, France), as previously described [13 (link)].
YM155, Necrostatin-1 and BAY11-7085 were purchased from Selleck Chemicals (Houston, USA) and the pan-caspase inhibitor Q-VD-OPh from R&DSystems (Abingdon, UK). Chloroquine, 3-Methyl-Adenin (3-MA), Bafilomycin A1 were purchased from Sigma-Aldrich (Saint-Quentin Yvelines, France). AS602868 was a generous gift of Merck-Serono International SA [14 (link)]. Antibodies against Survivin was purchased from R&DSystems (Lille, France), antibodies against LC3, p21, pS15-p53, pS317-Chk1, pT68-Chk2, IKK2, Actin, γH2AX from Merck Millipore (Saint-Quentin en Yvelines, France), antibodies against HSP90, p53 and cleaved (i.e. activated) caspase3 from Becton Dickinson (Pont de Claix, France), antibody against BAX from Dako (Courtaboeuf, France), and antibody against p62 from Santa Cruz (Heidelberg, Germany).
YM155, Necrostatin-1 and BAY11-7085 were purchased from Selleck Chemicals (Houston, USA) and the pan-caspase inhibitor Q-VD-OPh from R&DSystems (Abingdon, UK). Chloroquine, 3-Methyl-Adenin (3-MA), Bafilomycin A1 were purchased from Sigma-Aldrich (Saint-Quentin Yvelines, France). AS602868 was a generous gift of Merck-Serono International SA [14 (link)]. Antibodies against Survivin was purchased from R&DSystems (Lille, France), antibodies against LC3, p21, pS15-p53, pS317-Chk1, pT68-Chk2, IKK2, Actin, γH2AX from Merck Millipore (Saint-Quentin en Yvelines, France), antibodies against HSP90, p53 and cleaved (i.e. activated) caspase3 from Becton Dickinson (Pont de Claix, France), antibody against BAX from Dako (Courtaboeuf, France), and antibody against p62 from Santa Cruz (Heidelberg, Germany).
3
Cell lines for BRCA2 and SIRT studies
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DLD1 BRCA2+/+ and –/– (Horizon Discovery), HAP1 WT, SIRT1–/–, SIRT3–/–, SIRT6–/– (Horizon Discovery), CAL51 (DSMZ), HEK293T, MDA-MB-436 (ATCC) and U2OS WT and U2OS HPF1–/– (gift from Ivan Ahel) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS (with 10 µg/ml insulin in the case of MDA-MB-436). SUM149 cells (Asterand Bioscience) were maintained in Ham’s F-12 medium supplemented with 5% FBS, 10 µg/ml insulin and 1 µg/ml hydrocortisone.
All human cell line identities were confirmed by STR typing and verified free of mycoplasma infection using Lonza MycoAlert.
All human cell line identities were confirmed by STR typing and verified free of mycoplasma infection using Lonza MycoAlert.
4
Breast Cancer Cell Line Culturing
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Human breast cancer cell line MDA-MB-231, MDA-MB-453, BT-20, BT-549 and HCC1954 purchased from ATCC were cultured in RPMI 1640 or DMEM medium (Gibico) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% pennicilin/streptornycin (P/S, Beyotime, Shanghai). MFM-223, EFM-192A and CAL-51 were purchased from DSMZ and cultured in DMEM medium supplemented with 10% FBS and 1% P/S. SUM149 and SUM159 got from Asterland Bioscience were cultured in F12 medium (Gibico) with 5% FBS, 1% P/S, 5ug/ml insulin and 1ug/ml hydrocortisone (both from Sigma Aldrich). All of the cell lines were tested and authenticated. All cells were cultured at 37°C in a humidified atmosphere containing 5% CO2. The cell lines were mycoplasma-free and authenticated by PCR analysis monthly, and all cell lines were performed the STR authentication.
5
Characterization of Mammary Cancer Cell Lines
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The 4T1 mouse adenocarcinoma cell line was from ATCC (ref CRL-2539).
Regarding human cell lines, Cal51 and MDA-MB468 were from DSMZ GmbH, whereas HS578T, MCF10A, T47D, MCF-7, CAMA-1 , MDA-MB231 and MDA-MB157 were from ATCC. All human cell lines (including bclx KO cells) were authenticated by single nucleotide polymorphism profiling (Multiplexion GmbH). Cell lines were routinely tested for mycoplasma contamination using Mycoalert kit (Lonza).
HS578T and MDA-MB231 human mammary cancer cell lines were cultured in DMEM Glutamax (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin, at 37°C under a 5% CO 2 damp atmosphere.
The following antibodies were used: Bcl-xL (B22630-050, BD), Tubulin (SC-32293, Santa Cruz), Vinculin (SC-55465, Santa Cruz), mouse Flag (F3165, Sigma), rabbit Flag (F7425, Sigma), Calnexin (2679, Cell Signaling), F0F1 ATPase (612518, BD Biosciences), and Bcl-2 (Ab194583, Abcam).
Regarding human cell lines, Cal51 and MDA-MB468 were from DSMZ GmbH, whereas HS578T, MCF10A, T47D, MCF-7, CAMA-1 , MDA-MB231 and MDA-MB157 were from ATCC. All human cell lines (including bclx KO cells) were authenticated by single nucleotide polymorphism profiling (Multiplexion GmbH). Cell lines were routinely tested for mycoplasma contamination using Mycoalert kit (Lonza).
HS578T and MDA-MB231 human mammary cancer cell lines were cultured in DMEM Glutamax (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin, at 37°C under a 5% CO 2 damp atmosphere.
The following antibodies were used: Bcl-xL (B22630-050, BD), Tubulin (SC-32293, Santa Cruz), Vinculin (SC-55465, Santa Cruz), mouse Flag (F3165, Sigma), rabbit Flag (F7425, Sigma), Calnexin (2679, Cell Signaling), F0F1 ATPase (612518, BD Biosciences), and Bcl-2 (Ab194583, Abcam).
6
Cell Culture Conditions for Cancer Research
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A375, B16F10, EMT6, MIA PACA2, MDA‐MB‐468, HMCB, 4T‐1, LLC, and PAN02 cells were obtained from ATCC. CAL51 cells were obtained from DSMZ. CAL51, LLC, and PAN02 cells were cultured in DMEM with 10% FBS and 1% P/S. MIA PACA2 cells were cultured in DMEM supplemented with 15% FBS and 2.5% horse serum and 1% P/S. A375 cells were cultured in DMEM with 10% FBS and 1% P/S. EMT6 cells were cultured in Waymouth MB752 with 15% FBS, 2 mM L‐glutamine, and 1% P/S. All cells were cultured at 37°C in an incubator containing 5% CO2.
7
Cell line cultivation and maintenance
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Cell culture media were obtained from Invitrogen (Carlsbad, CA). MDA-MB-468, Cal51, 293 T, and 101 L hepatoma cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% Gibco Fetal Bovine Serum (FBS, Invitrogen) at 37°C and 5% CO2. MDA-MB-468shAhR and Cal51shAhR were maintained in DMEM with 10% Tet-System Approved FBS (Clontech) at 37°C and 5% CO2. MDA-MB-468 cells are mammary adenocarcinoma cells from a pleural effusion and were purchased from ATCC (Manassas, VA). Cal51 cells are also mammary adenocarcinoma cells from a plural effusion, but they exhibit a normal karyotype [31 (link)]. Cal51 was purchased from DSMZ (Braunschweig, Germany). 101 L hepatoma cells harbor a stably transfected luciferase reporter driven by three upstream DREs, and were obtained from Dr. Christopher Bradfield (Madison, WI), initially acquired from the laboratory of Dr. Robert Tukey (San Diego, CA) [32 (link)]. Parental cell lines were maintained in our laboratory for less than six months after resuscitation.
8
Breast Cancer Cell Line Sourcing
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CAL-51 was got from DSMZ (Germany). BT-549 was acquied from the cell bank of the Chinese Academy of Sciences (Shanghai, China). Professor Joyce Slingerland (University of Miami) generously provided MDA-MB-231/4175. The remaining breast cancer cell lines were obtained from the ATCC (Manassas, VA, US). All cells used in this study were cultured as suggested and identified by STR sequence analysis.
9
Culturing TNBC, Lung, and Colon Cancer Cell Lines
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TNBC cell line CAL-51 was grown in DMEM (Gibco) supplemented with 20% fetal bovine serum (FBS, Serana), 1% penicillin-streptomycin (P/S, Gibco) and 2mM L-glutamine (Gibco). TNBC cell line CAL-120 was grown in DMEM supplemented with 10% FBS, 1% P/S and 2 mM L-glutamine. TNBC cell line HCC1806, lung cancer cell lines A549 and H2122, colon cancer cell lines DLD-1 and RKO were grown in RPMI (Gibco) supplemented with 10% FBS, 1% P/S and 2 mM L-glutamine. TNBC cell line SUM159 was grown in DMEM/F12 (Gibco) supplemented with 10% FBS, 1% P/S, 5 μg/ml insulin (Sigma-Aldrich) and 1μg/ml hydrocortisone (Sigma-Aldrich).
HCC1806, A549, RKO, H2122 and DLD-1 were purchased from ATCC. SUM159 was a gift from Metello Innocenti (NKI, Amsterdam). CAL-51 and CAL-120 were obtained from DSMZ. All cell lines were regularly tested for mycoplasma contamination using a PCR assay and STR profiled (Eurofins).
HCC1806, A549, RKO, H2122 and DLD-1 were purchased from ATCC. SUM159 was a gift from Metello Innocenti (NKI, Amsterdam). CAL-51 and CAL-120 were obtained from DSMZ. All cell lines were regularly tested for mycoplasma contamination using a PCR assay and STR profiled (Eurofins).
10
Comparative Analysis of Cancer Cell Lines
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The following cell lines were used: HCT‐116 (colon cancer, ATCC CCL‐247), SW480 (colon cancer, ATCC CCL‐228), BT‐20 (breast cancer, ATCC HTB‐19), Cal‐51 (breast cancer, DSMZ ACC 302), MDA‐MB‐231 (breast cancer, ATCC HTB‐26), and MDA‐MB‐157 (breast cancer, ATCC HTB‐24). Cells were grown at 37 °C in a humidified atmosphere containing 5% CO2 in 10% Fetal Bovine Serum (FBS)‐supplemented McCoy’s 5A (HCT‐116 cells), EMEM (BT‐20 cells), or DMEM medium (Cal‐51, MDA‐MB‐231, and MDA‐MB‐157) (Gibco, Waltham, MA, USA). HEKn (ATCC PCS‐200‐010) primary neonatal foreskin keratinocytes were cultured in Dermal Cell Basal Medium (ATCC PCS‐200‐030) without FBS but supplemented with a Keratinocyte Growth kit (ATCC PCS‐200‐040).
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