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Agilent genomic dna uls labeling kit

Manufactured by Agilent Technologies
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Sourced in Netherlands

The Agilent Genomic DNA ULS Labeling Kit is a product designed for the labeling of genomic DNA samples. The kit utilizes the Universal Linkage System (ULS) technology to facilitate the efficient incorporation of labels into DNA fragments, enabling their subsequent detection and analysis.

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Lab products found in correlation

Dneasy tissue kit, by Qiagen (1 mentions) Feature extraction software, by Agilent Technologies (1 mentions) Nanovue, by Avantor (1 mentions) Genomic workbench standard edition 6, by Agilent Technologies (1 mentions)

Spelling variants of this product

ULS Labeling kit (3 citations) ULS labeling (3 citations) Agilent Genomic DNA Labeling Kit (2 citations) Agilent DNA Labeling Kit (2 citations) The Agilent Genomic DNA kit (2 citations) Genomic DNA kit (2 citations)

3 protocols using agilent genomic dna uls labeling kit

1

Genome-wide DNA Copy Number Array for ERG Deletions

Cited 2 times
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To identify ERG deletions, genome-wide DNA copy number arrays were performed as described previously4 (link). Briefly, Agilent SurePrint G3 Hmn 4 × 180 K arrays (Agilent Technologies, Amstelveen, the Netherlands) were co-hybridized with 1 μg patient DNA labeled with ULS-Cy5 and 1 μg reference genomic DNA male pool (G147A, Promega, Leiden, the Netherlands) labeled with ULS-Cy3 (Agilent Genomic DNA ULS Labeling Kit). Using median log ratios, data were normalized using the CGHcall47 (link) version 2.14.0, centralized using CGHnormaliter48 (link) version 1.8.0, and segmented and called using CGHcall default settings (−1 for loss, 0 for diploid, 1 for gain and 2 for amplification) in R version 2.14.1.
Steeghs E.M., Bakker M., Hoogkamer A.Q., Boer J.M., Hartman Q.J., Stalpers F., Escherich G., de Haas V., de Groot-Kruseman H.A., Pieters R, & den Boer M.L. (2018). High STAP1 expression in DUX4-rearranged cases is not suitable as therapeutic target in pediatric B-cell precursor acute lymphoblastic leukemia. Scientific Reports, 8, 693.
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2

Copy Number Analysis Using Microarray

Cited 1 time
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Copy number analysis was performed using Agilent SurePrint G3 Hmn 4×180K arrays (Agilent Technologies, Amstelveen, the Netherlands) co-hybridized with 1 μg patient DNA labeled with ULS-Cy5 and 1 μg reference genomic DNA male pool (G147A, Promega, Leiden, the Netherlands) labeled with ULS-Cy3 (Agilent Genomic DNA ULS Labeling Kit). Copy number microarray data were normalized using median log ratio in the CGHcall [27 (link)] version 2.14.0, centralized using CGHnormaliter [28 (link)] version 1.8.0, and segmented and called using CGHcall default settings (−1 for loss, 0 for diploid, 1 for gain and 2 for amplification) in R version 2.14.1.
Boer J.M., Steeghs E.M., Marchante J.R., Boeree A., Beaudoin J.J., Berna Beverloo H., Kuiper R.P., Escherich G., van der Velden V.H., van der Schoot C.E., de Groot-Kruseman H.A., Pieters R, & den Boer M.L. (2016). Tyrosine kinase fusion genes in pediatric BCR-ABL1-like acute lymphoblastic leukemia. Oncotarget, 8(3), 4618-4628.
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3

Oligo Array CGH Analysis of Dedifferentiated Tumors

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High-resolution oligo array comparative genomic hybridization analysis was used for a subset of samples of 12 tumors from 9 patients, including three usual (nos. 1, 2, and 6), five malignant (nos. 8, 9, 10, 22a, and 23a), and four dedifferentiated tumors (nos. 22b, 23b, 24a, and 24b). The samples taken from three patients (nos. 22, 23, and 24) were from the primary (no. 22a) or the earliest available lesion (Nos. 23a and 24a) and the subsequent dedifferentiated recurrence (nos. 22b, 23b, and 24b). Genomic DNA was extracted from the frozen samples by using a DNeasy Tissue Kit (Qiagen, Valencia, CA, USA) including RNase treatment, and its quality was assessed by using NanoVue (VWR) and agarose gel electrophoresis. The molecular karyotype of the samples was assessed by using an Agilent 144K array and an Agilent Genomic DNA ULS labeling kit in accordance with the manufacturer's protocol, and the raw data were processed using 'Feature extraction' software (Agilent Technologies, Santa Clara, CA, USA).
The data were analyzed using Agilent's Genomic Workbench Standard Edition 6.5.0.58. Copy-number variations, low-level copy-number abnormalities (0.3 olog2 ratio4 -0.3), and copy-number abnormalities involving chromosomes X and Y were not considered.
Dagrada G.P., Spagnuolo R.D., Mauro V., Tamborini E., Cesana L., Gronchi A., Stacchiotti S., Pierotti M.A., Negri T, & Pilotti S. (2015). Solitary fibrous tumors: loss of chimeric protein expression and genomic instability mark dedifferentiation. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 28(8).
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