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2 protocols using hc 030031 hc

1

Characterization of Lipid Modulators

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Allylisothiocyanate (AITC), carvacrol (Car), arachidonic acid (AA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), HC-030031 (HC) and A-967079 (A-9) and other reagents except where indicated, were obtained from Sigma Aldrich (Poole, UK). For in vitro experiments, stock solutions (x1000 working concentration) of AITC, AA, EPA and DHA were made in DMSO, and carvacrol was made in ethanol. Compounds were diluted to working concentrations on the day of use. Respective vehicle controls were used to confirm the specificity of each compound tested.
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2

Calcium Signaling in Cell Lines

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14,15-EET and 14,15-EE5ZE were from Cayman (Ann Arbor, MI). Quest Fluo-8 NW Calcium Assay Kit was from AAT Bioquest (AAT Bioquest Inc., Sunnyvale, CA, USA). Ca2+ free medium, capsaicin, capsazepine (CPZ), SB366791, HC030031 (HC), 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxymethyl ester (BAPTA/AM), ethylene glycol tetraacetic acid (EGTA), Griess's reagent, and Drabkin's reagent kit 525 were from Sigma-Aldrich (St. Louis, MO, USA). TurboFect was from Fermentas (Glen Burnie, MD, USA). Matrigel was from BD Biosciences (San Jose, CA, USA).
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