Kidneys were washed in phosphate-buffered saline (PBS) three times and fixed in a 4:1 mixture of absolute ethanol and 40% formaldehyde, then embedded in paraffin. Serial sections were made and hematoxylin–eosin (HE) staining (Sigma-Aldrich, MO, United States) for morphological analysis and Congo red staining (Abcam, Cambridge, United Kingdom) for Aβ accumulation were performed. The staining protocols were carried out according to the manufacturer’s instructions. Photomicrographs were taken using a DP74 camera (Olympus Corporation, Tokyo, Japan) on an Olympus Bx53 microscope (Olympus Corporation, Tokyo, Japan). For measurement of the staining intensity, we used ImageJ 1.40g freeware. Pixel density analysis was performed from at least three photos of five independent experiments.
Hematoxylin eosin staining
Manufactured by Merck Group
Sourced in United States, Germany
Hematoxylin/eosin staining is a common histological staining technique used in pathology and microscopy. The stain consists of two components: hematoxylin, which stains nuclei blue, and eosin, which stains cytoplasm and other structures pink or red. This staining method provides contrast to facilitate the visualization and differentiation of various cellular and tissue structures under a microscope.
Automatically generated - may contain errors
Lab products found in correlation
Roti histofix 4, by Carl Roth (2 mentions)
Eclipse 80i microscope, by Nikon (2 mentions)
Dp74 camera, by Olympus (1 mentions)
Isopentane, by Merck Group (1 mentions)
Mtp slide adapter, by Bruker (1 mentions)
Ultraflextreme maldi tof tof mass spectrometer, by Bruker (1 mentions)
Ab208670, by Abcam (1 mentions)
Ab 203189, by Abcam (1 mentions)
Masson s trichrome staining kit, by Merck Group (1 mentions)
Sirius red staining, by Merck Group (1 mentions)
9 protocols using hematoxylin eosin staining
1
Histological Analysis of Kidney Tissue
2
MALDI-TOF MS Brain Proteomics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Brains (n=4) were isolated and immediately snap frozen in isopentane (Sigma, USA) at - Ethanol and then stored in a dessicator under vacuum. In order to be analyzed by MALDI TOF MS, samples were covered with sinapinic acid (10mg/mL in 60% acetonitrile and 0.2% trifluoroacetic acid (TFA)) as MALDI matrix sprayed using
ImagePrep device (Bruker Daltonics). The device parameters were optimized for the brain sections, with a final set-up of 20 cycles combining 2,5s of spray, 5s of incubation and 20s of dry time. Finally the slides were mounted into MTP slide adapter (Bruker Daltonics) and transferred to the MALDI-TOF mass spectrometer. Four biologically independent samples were analyzed by MS. IMS spectra were acquired in linear positive ion mode in an UltrafleXtreme MALDI-TOF/TOF mass spectrometer (Bruker Daltonics). MS data were averaged from 300 consecutive laser shots with a frequency of 2,000 shot/s. The raster spatial resolution was 100 µm.
After the MS analysis, hematoxylin & eosin (H&E) staining (Sigma) was performed in each brain section and they were used as reference for colocalization to select the regions of interest (ROI).
ImagePrep device (Bruker Daltonics). The device parameters were optimized for the brain sections, with a final set-up of 20 cycles combining 2,5s of spray, 5s of incubation and 20s of dry time. Finally the slides were mounted into MTP slide adapter (Bruker Daltonics) and transferred to the MALDI-TOF mass spectrometer. Four biologically independent samples were analyzed by MS. IMS spectra were acquired in linear positive ion mode in an UltrafleXtreme MALDI-TOF/TOF mass spectrometer (Bruker Daltonics). MS data were averaged from 300 consecutive laser shots with a frequency of 2,000 shot/s. The raster spatial resolution was 100 µm.
After the MS analysis, hematoxylin & eosin (H&E) staining (Sigma) was performed in each brain section and they were used as reference for colocalization to select the regions of interest (ROI).
3
Liver Injury Assessment Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To ensure that BDL caused significant injury to the liver, ALT, AST and total bilirubin were measured as part of the biochemical analysis. In addition, a histological work-up of the liver tissue was performed. Liver sections were fixed in 4% buffered formalin (ROTI Histofix 4%, Carl Roth GmbH + Co. KG, Karlsruhe, Germany) for one week, subsequently embedded in paraffin and cut into slices of 3 μm thickness. The prepared slides were then dewaxed and rehydrated using a standard xylol and a descending alcohol series. Histological examination of liver tissue was performed by means of hematoxylin & eosin staining (Merck KGaA, Darmstadt, Germany). HE staining was conducted according to a standard protocol.
4
Immunohistological Analysis of Rat Myocardial Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Midpapillary sections of rat hearts were fixed in 4% buffered formalin (ROTI Histofix 4%, Carl Roth GmbH + Co. KG, Karlsruhe, Germany) for one week, subsequently embedded in paraffin and cut into slices of 3 μm thickness. The prepared slides were then dewaxed and rehydrated using a standard xylol and a descending alcohol series. Histological examination of the myocardial tissue was performed by means of hematoxylin & eosin staining (Merck KGaA, Darmstadt, Germany) and elastic Van Gieson staining (Merck KGaA, Darmstadt, Germany; Chroma, Waldeck GmbH & Co. KG, Muenster, Germany). All stains were conducted according to a standard protocol.
For immunohistological staining, the ZytoChem-Plus AP Polymer-Kit (Zytomed Systems GmbH, Berlin, Germany) was used according to the manufacturer’s protocol. To detect microthrombi, cluster of differentiation 41 (CD41) antibody (ab 203189, Abcam, Berlin, Germany) and neutrophil myeloperoxidase (MPO) antibody (ab 208670, Abcam, Berlin, Germany) were used as primary antibodies. The evaluation of the stained slides was performed in collaboration with the Department of Pathology, RWTH Aachen University, Faculty of Medicine, Aachen, Germany.
For immunohistological staining, the ZytoChem-Plus AP Polymer-Kit (Zytomed Systems GmbH, Berlin, Germany) was used according to the manufacturer’s protocol. To detect microthrombi, cluster of differentiation 41 (CD41) antibody (ab 203189, Abcam, Berlin, Germany) and neutrophil myeloperoxidase (MPO) antibody (ab 208670, Abcam, Berlin, Germany) were used as primary antibodies. The evaluation of the stained slides was performed in collaboration with the Department of Pathology, RWTH Aachen University, Faculty of Medicine, Aachen, Germany.
5
Histological Analysis of Mouse Hearts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Histology assays were performed with hearts and sections as previously described [22 (link)]. The mouse heart tissues were collected and fixed with 4% paraformaldehyde. Tissues were processed as paraffin section and subsequently analyzed by hematoxylin-eosin staining according to the manufacturer's protocol (Sigma-Aldrich). The sections were imaged by microscopy.
6
Histochemical Analysis of Melanoma Tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On the 20th day after melanoma cells inoculation mice from all groups were sacrificed by cervical dislocation and tumors were collected for histochemical analysis. The collected tumors were embedded in OCT (Leica Biosystems, Wetzlar, Germany), frozen in liquid nitrogen and stored at −80 °C until needed. Subsequently, frozen tumors were sectioned into 5 μm slices. The frozen sections were examined histochemically (hematoxylin/eosin staining, Sigma-Aldrich, St. Louis, MO, USA), and the analysis of the specimens was conducted using the Nikon Eclipse 80i microscope (Nikon Instruments Inc., Tokyo, Japan).
7
Quantification of Cardiac Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LV sections were fixed in a 10% formalin solution, dehydrated in ethanol, and then embedded in paraffin as previously described36 (link). Tissue sections (5 μm) were obtained in a microtome, were deparaffinized, rehydrated, and stained with Masson’s trichrome staining kit (EMD Millipore Corporation, Billerica, MA, USA) and Sirius red staining (Sigma- Aldrich, San Luis, MI, USA) for fibrosis quantification. Hematoxylin/Eosin staining was performed to explore heart morphology (Sigma-Aldrich, San Luis, MI, USA).
8
Immunohistochemical Analysis of Muscle Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
One, 3, 7, 14, and 21 days after the surgery, mice were sacrificed by cervical dislocation and the gastrocnemius muscles were collected for immunohistochemical analysis. The collected muscles were frozen in liquid nitrogen and stored at − 80 °C until needed. Subsequently, frozen tissues were sectioned into 5-μm slices.
The frozen sections were examined histochemically (hematoxylin/eosin staining, Sigma Aldrich), and the analysis of the specimens was conducted using the Nikon Eclipse 80i microscope (Nikon Instruments Inc., Melville, NY, USA).
The frozen sections were examined histochemically (hematoxylin/eosin staining, Sigma Aldrich), and the analysis of the specimens was conducted using the Nikon Eclipse 80i microscope (Nikon Instruments Inc., Melville, NY, USA).
9
Tooth Section Histological Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tooth sections were deparaffinized and rehydrated. Nestin and DSP staining were performed as described previously (18) . Hematoxylin-eosin staining (Sigma-Aldrich, St Louis, MO) was performed on tooth sections according to the manufacturer's instructions. Finally, the slides were analyzed by an operator blinded to the groups under a light microscope and classified using the following criteria:
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!