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Anti usp1

Manufactured by Cell Signaling Technology
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Sourced in United States, United Kingdom

Anti-USP1 is a lab equipment product from Cell Signaling Technology. It is an antibody that specifically recognizes the USP1 protein. USP1 is a deubiquitinating enzyme that plays a role in DNA repair processes.

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Lab products found in correlation

Anti p27, by Cell Signaling Technology (2 mentions) Anti ubiquitin, by Cell Signaling Technology (1 mentions) Anti usp14, by Cell Signaling Technology (1 mentions) Anti uchl3, by Cell Signaling Technology (1 mentions) Anti skp2, by Cell Signaling Technology (1 mentions) Anti stat5, by Cell Signaling Technology (1 mentions) Anti a20, by Cell Signaling Technology (1 mentions) Anti phospho stat5, by Cell Signaling Technology (1 mentions) Anti crkl, by Cell Signaling Technology (1 mentions) Anti uchl1, by Cell Signaling Technology (1 mentions) Co ip assay kit 14311d, by Thermo Fisher Scientific (1 mentions) Anti k48 ubiquitin, by Cell Signaling Technology (1 mentions) Anti usp10, by Cell Signaling Technology (1 mentions) Anti usp2, by Cell Signaling Technology (1 mentions) Anti usp7, by Cell Signaling Technology (1 mentions) Anti usp8, by Cell Signaling Technology (1 mentions) Anti usp15, by Cell Signaling Technology (1 mentions) Anti usp18, by Cell Signaling Technology (1 mentions) Anti cyld, by Cell Signaling Technology (1 mentions) Anti c abl, by Cell Signaling Technology (1 mentions)

3 protocols using anti usp1

1

Ubiquitin-mediated protein regulation assay

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Spautin-1 (S7888), Imatinib (S1026), and MG132 (S2619) were from Sellectchem (Houston, TX, USA). SKP2-C25 (M60136) was from Xcessbio Biosciences, Inc. (San Diego, CA). MTS reagent (G3582) was from Promega Corporation (Madison, WI, USA). Co-IP assay kit (14311D) was from Life Technologies (Carlsbad, CA). Antibodies: anti-Ubiquitin (#3936), anti-K63-Ubiquitin (#12930), anti-K48-Ubiquitin (#12805), anti-USP10 (#8501), anti-USP1 (#8033), anti-USP2 (#8036), anti-USP7 (#4833), anti-USP8 (#11832), anti-USP14 (#11931), anti-USP15 (#66310), anti-USP18 (#4813), anti-UCHL1 (#13179), anti-UCHL3 (#8141), anti-CYLD (#8462), anti-A20 (#5630), anti-SKP2 (#2652), anti-p27 (#3686), anti-FLAG (#14793), anti-HA (#3724), anti-phospho-c-Abl(Y245) (#2861), anti-c-Abl (#2862), anti-phospho-STAT5 (#9359), anti-STAT5 (#25656), anti-phospho-Crkl (#3181) and anti-Crkl (#38710) (Cell Signaling Technology, Beverly, MA, USA); anti-UCHL5 (ab124931), anti-USP13 (ab109264) (Abcam, Cambridge, MA); anti-GAPDH (BS60630) (Bioworld Technology, Inc., Louis Park, MN, USA).
Liao Y., Liu N., Xia X., Guo Z., Li Y., Jiang L., Zhou R., Tang D., Huang H, & Liu J. (2019). USP10 modulates the SKP2/Bcr-Abl axis via stabilizing SKP2 in chronic myeloid leukemia. Cell Discovery, 5, 24.
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2

Western Blot Analysis of Protein Targets

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Cells were lysed using RIPA buffer (Beyotime, Nantong, China) containing 1 mM phenylmethylsulfonyl fluoride (Beyotime). Protein was loaded and separated by 8% or 12% SDS-PAGE gels, transferred to polyvinylidene fluoride membranes (Millipore, Darmstadt, Germany), and blocked with bovine serum albumin (5%; Sangon Biotech, Shanghai, China). The primary antibodies included anti-USP1 (1:1000; Cell Signaling Technology), anti-TBLR1 (1:1000; Abcam, Cambridge, United Kingdom), anti-HA (1:2000; Proteintech, Wuhan, China), anti-β-actin (1:5000; Cell Signaling Technology). Secondary antibodies included donkey anti-rabbit (1:2500; Cell Signaling Technology), rabbit anti-mouse (1:2500; Cell Signaling Technology), and anti-light chain (1:3000; Abcam).
Li Y., Xu Y., Gao C., Sun Y., Zhou K., Wang P., Cheng J., Guo W., Ya C., Fan J, & Yang X. (2020). USP1 Maintains the Survival of Liver Circulating Tumor Cells by Deubiquitinating and Stabilizing TBLR1. Frontiers in Oncology, 10, 554809.
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3

Kinase Inhibitor Library Evaluation

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A custom-built kinase inhibitor library (partial class of kinase inhibitors selected by authors), SNS-032 (#S1145), Dinaciclib (#S2768), Cycloheximide (#S7418), MG132 (#S2619), Bafilomycin A1 (#S1413), bortezomib (#S1013), and enzalutamide (#S1250) were obtained from Selleckchem (Houston, TX). Antibodies: anti-USP1 (#8033), anti-SIX1 (#16960), anti-Cyclin D1 (#55506), anti-p21 (#2947), anti-p27 (#3686), anti-cleaved Caspase 3 (#9661), anti-FLAG-tag (#8146), anti-Ki-67 (#9449), anti-AR (#5153), and anti-AR-V7 (#19672) were from Cell Signaling Technology (Beverly, MA); anti-GAPDH (#ab181602) were from Abcam (Cambridge, MA).
Liao Y., Sun W., Shao Z., Liu Y., Zhong X., Deng Y., Liu F., Huang H, & Liu J. (2022). A SIX1 degradation inducer blocks excessive proliferation of prostate cancer. International Journal of Biological Sciences, 18(6), 2439-2451.
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