Bsa control

For cell viability experiments, cells were treated with a range of concentrations of the indicated small molecule compounds, and relative viability was measured by the CellTiter-Glo Assay. Cells were seeded in 96-well black-wall tissue culture-treated plates at 2,000 to 3,000 cells per well (or 10,500 for primary human monocytes and macrophages), to reach 30% confluency the following day. Cells were treated with compounds 18–24 h after seeding using an HP D300e Digital Dispenser with three biological replicates per condition. For BSA-palmitate and BSA-control experiments, compounds were added manually to cells. 66–72 h after compound treatment, viability was measured using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) as per the manufacturer’s instructions. Unless otherwise specified, relative viability was normalized to the untreated condition. Regression fit curves were computed in Prism 8 or 9 (GraphPad) using four-parameter inhibition nonlinear regression. The mean and standard deviation for three biological replicates of each data point were calculated. The following compounds were used: RSL3 (Selleck Chem), ML210 (Sigma Aldrich), erastin (Selleck Chem), FIN56 (Selleck Chem), Ferrostatin-1 (Sigma Aldrich), Z-VAD-FMK (Selleck Chem), Necrostatin-1 (Selleck Chem), BSA-palmitate (Cayman Chem), BSA-control (Cayman Chem).