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2 protocols using methyltrioctylammonium chloride

1

Sugarcane Bagasse Pretreatment for Biorefinery

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Sugarcane bagasse (SB) was procured from a local sugar industry. It was ground to 300 µ pore size and termed as untreated SB (UTB). It was pretreated sequentially with sodium hydroxide and Methyltrioctylammonium chloride (IL) as mentioned by Ejaz et al. [12 (link)] and referred as pretreated SB (PTB). Methyltrioctylammonium chloride (≥90%, under the trade name of Aliquat® 336) was purchased from Sigma-Aldrich (Burlington, NJ, USA) and used without any purification.
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2

Daunorubicin Extraction Protocol

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Ethylene glycol (EG), (1S)-(+)-camphor-10-sulfonic acid (CSA), p-aminophenol, salicylic acid (SA), methyltrioctylammoniumchloride (MTOAC), n-butanoic acid (BA), 1-undecanol, l-menthol, 1-octanol, thymol and pure standard of daunorubicin hydrochloride (purity higher than 95 %) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetone, acetonitrile (for spectroscopy), methanol (HPLC grade), sodium dodecyl sulfate (SDS), decanoic acid, 1-decyl-3-methylimidazolium chloride ([DMIM]Cl), Tetrabutyl ammonium chloride (TBAC), Na2HPO4, NaCl, KOH and HCl (37 %) were purchased from Merck (Darmstadt, Germany). A stock solution of DNR at 1000 mg L−1 was prepared in 1 % (v/v) formic acid in water:methanol (50:50 % v/v). Working solutions were prepared by daily dilution of the stock solution.
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