Protein was obtained from cells and Western blot analyses were performed as reported previously [52 (link),55 (link)]. Primary antibody against all denatured HLA-G was detected with MEM-G/1 (Exbio) [35 (link)], with primary antibody against α-tubulin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used as internal control [52 (link)].
Primary antibody against α tubulin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The primary antibody against α-tubulin is a laboratory reagent used to detect and visualize the α-tubulin protein in biological samples. α-tubulin is a component of the cytoskeletal microtubules, which are essential for various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to study the distribution and expression of α-tubulin in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Mem g 1, by Exbio (1 mentions)
Ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
X ray film, by Kodak (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Proteinase inhibitor cocktail, by Roche (1 mentions)
Nupage 4 12 bis tris precast gel, by Thermo Fisher Scientific (1 mentions)
2 protocols using primary antibody against α tubulin
1
HLA-G Protein Detection via Western Blot
2
Immunoblotting of ELP1 Protein
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Protein samples were obtained by tissue lysis with ice-cold RIPA buffer (Sigma) and Proteinase Inhibitor Cocktail (Roche). Only 50 µg of total protein was separated on NuPAGE 4–12% Bis-Tris precast gels (ThermoFisher) and transferred to nitrocellulose membranes. The membranes were blocked for 1 h in 5% non-fat milk and successively blotted overnight at +4°C in 5% milk with a primary antibody against ELP1 (1:500; Anaspec) and a primary antibody against α-tubulin (1:5000; Santa Cruz Biotechnology). Membranes were washed and incubated with specific secondary antibodies for 1 h at room temperature (anti-Rabbit 1:2000 and anti-mouse 1:5000). Protein bands were detected with ECL western blotting substrate (ThermoFisher) followed by exposure to X-ray film (Kodak).
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