Anti pten

Antibody against p-CDK1 (Thr 161) was purchased from Abcam (Cambridge, MA, USA). Anti-p85α, anti-Akt, and p-Akt (Ser 473) antibodies were purchased from BD PharMingen. Antibodies against p21, CDK1, CDK2, cyclin A, cyclin B1, cyclin D, cyclin E, and p110α were purchased from Santa Cruz Biotechnology. Anti-pan-cadherin, anti-PTEN, and anti-PTEN (Ser 380/Thr 382/385) antibodies were obtained from Thermo Fisher Scientific (New York, NY, USA). Antibodies against β-actin, hemagglutinin (HA)-epitope tag, and FLAG-epitope tag were obtained from Sigma-Aldrich. Peroxidase-conjugated anti-mouse IgG, anti-goat IgG, and anti-rabbit IgG secondary antibodies were purchased from Jackson ImmunoResearch Laboratory (West Grove, PA, USA).

Antibodies against caspase-3, caspase-9, and caspase-12 were obtained from Calbiochem (San Diego, CA, USA). Anti- p85α -Akt, -phospho (p)-Akt (Ser 473), BAX, BAK, BCL-2, BCL-_XL, and cytochrome c (Cyt c) antibodies were purchased from BD PharMingen. Antibodies directed against acid sphingomyelinase (ASM), BAD, p-BAD (Ser 128), p-BCL-2 (Ser 87), Bid, calnexin, p-CDK1 (Thr 161), Cyt c oxidase subunit II (Cox-2), and MCL-1 were obtained from Abcam (Cambridge, MA, USA). Antibodies against CD55, CD71, nucleolin, p21, CDK1, CDK2, cyclin A, cyclin B1, cyclin D, cyclin E, p110α, poly (ADP-ribose) polymerase (PARP), and Rac1 were purchased from Santa Cruz Biotechnology. Anti-pan-cadherin, anti-PTEN, and anti-p-PTEN (Ser 380/Thr 382/Ser 385) antibodies were obtained from Thermo Fisher Scientific (New York, NY, USA). Antibodies against β-actin, p-BCL-2 (Thr 69), p-BCL-_XL (Ser 62), FLAG-epitope tag, and α-tubulin were obtained from Sigma-Aldrich. Horseradish peroxidase (HRP)-conjugated streptavidin was provided from Thermo Fisher Scientific (New York, NY, USA). HRP-conjugated anti-mouse, -goat, and -rabbit IgG secondary antibodies were purchased from Jackson ImmunoResearch Laboratory (West Grove, PA, USA).

Antibodies against MMP-1 and MMP-9 were purchased from Oncogene Research Product (Cambridge, MA, USA). Antibody against caspase-3 was obtained from Calbiochem (San Diego, CA, USA). Anti-Akt, -phospho (p)-Akt (Ser 473), p-Akt (Thr 34), and -NF-κB p65 antibodies were purchased from BD PharMingen. Anti-p-NF-κB (Ser 536) antibody was obtained from Cell Signaling Technologies (Boston, MA, USA). Antibodies directed against ASM and calnexin were obtained from Abcam (Cambridge, MA, USA). Antibodies against CD55, CD71, nucleolin, p110α, poly (ADP-ribose) polymerase (PARP), and Rac1 were purchased from Santa Cruz Biotechnology. Anti-pan-cadherin, anti-PTEN, and anti-p-PTEN (Ser 380/Thr 382/Ser 385) antibodies were obtained from Thermo Fisher Scientific (New York, NY, USA). Antibody against β-actin was obtained from Sigma-Aldrich. Peroxidase-conjugated anti-mouse, -goat, and -rabbit IgG secondary antibodies were purchased from Jackson ImmunoResearch Laboratory (West Grove, PA, USA).