The Chinese hamster ovary CHO cell line (CHO-K1, RCB0403, Riken Bio Resource Center, Ibaraki, Japan) was used as a representative adherent cell line, as CHO cells are commonly used for protein production46 (link). The CHO cells were cultured in serum-supplemented Ham’s F-12 medium (α-MEM Ham’s F-12, Wako, Tokyo, Japan) supplied with 10% fetal bovine serum (FBS, S1820, Biowest SAS, Nuaillé, France) in a 5% CO2 humidified atmosphere incubator at 37 °C. Cell passage was performed by trypsinization with 0.050% trypsin-EDTA (25300, Life Technologies, CA, USA) by pipetting.
The CHO cells were passaged the minimal number of times needed for experiments and were seeded in cell culture dishes. Cells seeded in the 2 mL SSM were incubated for 24–48 h in a 5% CO2 humidified atmosphere incubator at 37 °C and then used for cell detachment experiments.
The CHO cells were passaged the minimal number of times needed for experiments and were seeded in cell culture dishes. Cells seeded in the 2 mL SSM were incubated for 24–48 h in a 5% CO2 humidified atmosphere incubator at 37 °C and then used for cell detachment experiments.