1290 uplc

Manufactured by Agilent Technologies

Sourced in United States

The 1290 UPLC is an ultra-high-performance liquid chromatography (UPLC) system designed for efficient and high-resolution separation of complex samples. It features a robust and compact design, providing reliable performance and accuracy in analytical laboratories.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (3 mentions) Qtof 6550, by Agilent Technologies (2 mentions) 2 chlorophenylalanine, by Thermo Fisher Scientific (1 mentions) Q tof ms 6550, by Agilent Technologies (1 mentions) 6545 q tof, by Agilent Technologies (1 mentions) Qtof ms 6540, by Agilent Technologies (1 mentions) 6460 qqq ms, by Agilent Technologies (1 mentions) Ms 5000 spectrometer, by Bruker (1 mentions) Analyst tf 1, by AB Sciex (1 mentions) Enzyme linked immunosorbent assay kit, by MultiSciences Biotech (1 mentions) Ix73p2f, by Olympus (1 mentions) C1 si te2000, by Nikon (1 mentions) Model 550, by Bio-Rad (1 mentions) 1100 hplc, by Agilent Technologies (1 mentions) Milli q gradient water system, by Merck Group (1 mentions) Waters 600 hplc system, by Waters Corporation (1 mentions) Waters 2996 pda, by Waters Corporation (1 mentions) 400 mhz nmr, by Bruker (1 mentions) Prep c18 column, by Agilent Technologies (1 mentions) Ab nano lc 400, by AB Sciex (1 mentions)

Close spelling variants of this product

Agilent 1290 UPLC system (55 citations) 1290 Infinity UPLC system (29 citations) Agilent 1290 UPLC (26 citations) 1290 Infinity UPLC (21 citations) 1290 Infinity II UPLC (21 citations) Agilent 1290 Infinity UPLC (17 citations) Agilent 1290 Infinity UPLC system (15 citations) Agilent 1290 series UPLC system (14 citations) Agilent 1290 Infinity II UPLC system (7 citations) 1290 Infinity Binary UPLC (5 citations)

18 protocols using 1290 uplc

QTOF/MS-UPLC Metabolomics Analysis

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Mass Spectrometer (QTOF/MS-6550, Aglient), Ultra-High Performance Liquid Chromatograph (UPLC-1290, Aglient), Vortex mixer (MIX-200, Jingxin, Shanghai), centrifuge (5427R, Eibend, Germany), acetonitrile and methanol were bought from Merck (Darmstadt, Germany). Formic acid and 2-chlorophenylalanine were from Thermo Fisher. All chemicals were of chromatographic grade.

Li M., Guo K., He Y., Li H., Sun W., Yuan X., Liu Z., Li X., Merriman T.R., Li C, & Zhang H. (2024). Natural Changbai mineral water reduces obesity risk through regulating metabolism and gut microbiome in a hyperuricemia male mouse model. Frontiers in Nutrition, 11, 1308882.

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Comprehensive Lipidomic Profiling of Plasma

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The Agilent Qtof 6545 and UPLC 1290 assay detects about 700 distinct lipid species across 13 different lipid classes (e.g., lysophosphatidylcholines, lysophosphatidylethanolamines, phosphatidylcholines, phosphatidylethanolamines, sphingomyelins, diacylglycerols, triacylglycerols, free fatty acids, cholesterol, ceramides, dihydroceramides, hexosylceramides, and lactosylceramides). Each sample is run once on the platform using positive and negative ionization modes. Data acquisition lasts for 20 min per sample, and 25μL of the plasma equivalent will be injected. Data quality will be ensured by using a set of lipid standards after each cleaning, 24 hr of idling or 3 days of consecutive use, performing a quick system suitability test before each batch to ensure acceptable limit of detection for each lipid class, sample randomization for lipid extraction and data acquisition, and triplicate injection of lipids extracted from a reference plasma sample at the beginning of the batch.

Rowan N.J., Deans K., Anderson J.G., Gemmell C.G., Hunter I.S, & Chaithong T. (2001). Putative Virulence Factor Expression by Clinical and Food Isolates of Bacillus spp. after Growth in Reconstituted Infant Milk Formulae. Applied and Environmental Microbiology, 67(9), 3873-3881.

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UPLC-Q-TOF-MS Analysis of Drugs

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Chromatographic analysis was performed using an ultra-performance liquid chromatograph (UPLC 1290, Agilent Technologies, Germany). The separation was done employing a Poroshell 120 EC-C18 column 3.0 × 100 mm; 2.7 µm (Agilent Technologies, USA) with a thermostat at 40 °C. A mixture of 0.1 % formic acid in water (A) and 0.1 % formic acid in acetonitrile (B) was used as a mobile phase. Gradient elution was carried out at a constant fl ow of 0.4 mL min -1 . The following gradient was applied: 0 min 95 % A (5 % B), 0-5 min 30 % A (70 % B), 5-7 min 0 % A (100 % B) and then 7-8.5 min 100 % A (0 % B). Return to the starting gradient composition (95 % A and 5 % B) was performed at 4 min. The injected volume was 10 µL. Detection of the investigated compounds was achieved using a quadrupole coupled to time-of-fl ight analyzer (Q-TOF-MS 6540, Agilent Technologies, USA). The spectrometer was equipped with an ESI Jet Stream source; identifi cation and determination of the investigated drug was carried out in the SCAN mode. Operating spectrometer parameters are presented in Table I.

Szpot P, & Buszewicz G. (2015). Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionization. Acta pharmaceutica (Zagreb, Croatia), 65(2).

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Quantitative Analysis of Metabolites

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Frozen samples were centrifuged for 10 min at 4 °C at 20,817g to remove salts. The supernatant was removed and analyzed by LC–QQQ using MRM in positive ionization mode on an Agilent 6460 QQQ MS. Samples were analyzed on two tandem Ascentis Express RP-amide HPLC columns (2.7 μm, 2.1 mm × 10 cm, 25 °C; Supelco) on an Agilent 1290 UPLC using an isocratic solvent system of 10% acetonitrile in 0.1% (v/v) formic acid as the aqueous mobile phase for 20 min after an initial ramp of 0 to 10% acetonitrile over 0.5 min at flow rate of 0.4 ml min⁻¹. Products were monitored with transitions as follows (parent ion m/z → product ion m/z, fragmentor, collision energy): TKL (171 → 153, 90,6); H-TKL (157 → 139, 90, 4); F-TKL (175 → 157, 90, 5) and H-TTKP (199 → 111, 100, 9). The identity and the quantity of each compound was verified against synthetic standards using external standard curves. Synthetic standards were obtained as previously described^{28 (link)}.

Marsh B.J., Mastronarde D.N., Buttle K.F., Howell K.E, & McIntosh J.R. (2001). Organellar relationships in the Golgi region of the pancreatic beta cell line, HIT-T15, visualized by high resolution electron tomography. Proceedings of the National Academy of Sciences of the United States of America, 98(5), 2399-2406.

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Analytical Techniques for Organic Contaminants

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Organic contaminants were determined on a high-performance liquid chromatography (HPLC) system (1200, Agilent Technology, USA) equipped with a C18 column (4.6 × 250 mm, 5 μm). TPs of BPA were analyzed on an HPLC system (1290uplc, Agilent, USA) connected with a triple quadrupole mass spectrometer (QTOF6550, Agilent, USA), the details are provided in Supplementary Note 3. EPR spectra were obtained using MS-5000 spectrometer (Bruker, Germany) (Supplementary Note 7). Kinetic solvent isotope effect, FFA and DPA product detection was used to determine ¹O₂ production (Supplementary Note 8,9). Electrochemical characterization was performed using a CHI 760E electrochemical workstation equipped with a standard three-electrode system (Supplementary Note 14). The optimized geometry of BPA was optimized using the Gaussian 09 with a basis set of B3LYP/6–31G (d, p) and was visualized using Multiwfn^{60 (link)}. The aquatic toxicity of the transformation byproducts was predicted using the USEPA ECOSAR program^{26 (link)}.

Liu T., Xiao S., Li N., Chen J., Zhou X., Qian Y., Huang C.H, & Zhang Y. (2023). Water decontamination via nonradical process by nanoconfined Fenton-like catalysts. Nature Communications, 14, 2881.

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UPLC-QTOF Lipids Analysis Protocol

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Lipids analysis was performed with 1290 UPLC (Agilent, USA) equipped with Triple TOF 6600 (Q-TOF, AB Sciex, USA), using a Phenomen Kinetex C18 100A Column (100 × 2.1 mm, 1.7 μm). The mobile phase A was comprised 10 mM Ammonium acetate, 40% H₂O and 60% ACN, and the mobile phase B was comprised 10 mM Ammonium acetate, 10% CAN and 90% IPA. An elution gradient started with 40% B for 12 min; subsequently changed to 100% B within 1.5 min; at 13.7 min, changed to 40% B again. Run duration was 18 min with 300 μL/min. The injection volumes were 1 μL (positive mode) and 3 μL (negative mode). The Analyst TF 1.7 (AB Sciex, USA) constantly assessed the comprehensive scan survey MS data as it collected and touched off the acquisition of MS/MS spectra depending on preselected criteria. The program of ESI source: ion source gas 1 and 2 were 60, curtain gas was 30; the source temperature was held at 550°C, ion spray voltage floating was 5,500 and −4,500 V.

Li Q., Yang L., Li R., Chen G., Dong J., Wu L., Fu Y, & Yang J. (2023). Lipid analysis of meat from Bactrian camel (Camelus bacterianus), beef, and tails of fat-tailed sheep using UPLC-Q-TOF/MS based lipidomics. Frontiers in Nutrition, 10, 1053116.

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Multimodal Imaging and Analytical Techniques

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Scanning electron microscopy (SEM) images were recorded by a scanning electron microscope (Zeiss Sigma). In vivo imaging was conducted by the Spectrum Pre-clinical In Vivo Imaging System (IVIS, PekinEmer). The cell viability was measured by the microplate reader (Bio-Rad, Model550, USA). Blood routine analysis was examined by Auto Hematology Analyzer (MC-6200VET) and blood biochemistry analysis was conducted by biochemical auto analyzer (MNCHIP, Tianjin, China). Trans-epidermal water loss (TWEL) was measured by TEWL tewameter (TM300) (Cologne, Germany). Skin hydration, and skin elasticity were evaluated by Imate Skin Moisture Tester (M − 6602). Scratch wound-healing assay was carried out by fluorescence inverted microscope (Olympus IX73P2F). Mass spectrometry was performed by Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) (Agilent 1290 uplc and Agilent qtof 6550). Confocal microscopy images were recorded on a confocal laser scanning microscope (CLSM) (Nikon C1-si TE2000). Enzyme-linked immunosorbent assay kits were provided by Multi Science.

Liu X., Qin Y., Dong L., Han Z., Liu T., Tang Y., Yu Y., Ye J., Tao J., Zeng X., Feng J, & Zhang X.Z. (2022). Living symbiotic bacteria-involved skin dressing to combat indigenous pathogens for microbiome-based biotherapy toward atopic dermatitis. Bioactive Materials, 21, 253-266.

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Quantification of Oxidized Glutathione by HPLC-MS

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The oxidized glutathione (GSSG) diffusion experiment was performed on an ion trap (6340, Agilent Technologies). Analyses were performed using an isocratic flow (1100 HPLC, Agilent Technologies) of 50μl/min (50% A = 0.1% formic acid in water, 50% B = 0.1% formic acid in acetonitrile). The system was operated in positive electrospray ionization mode. In MS mode, the maximum accumulation time was 500 ms, scan range of 50-2200 m/z, and averages = 3. For multiple reaction monitoring (MRM), masses were selected and isolated for MS(n) with a width of 4.0. For E. coli, a direct injection of 30-592 cells (OD) generated a satisfactory signal in an injection volume of 2 μl. ESI-TOF analyses were performed using an isocratic flow (1290 UPLC, Agilent Technologies) of 50μl/min (50% A = 0.1% formic acid in water, 50% B = 0.1% formic acid in acetonitrile) coupled to a Q-TOF (6538, Agilent Technologies). The system was operated in positive electrospray ionization mode, using extended dynamic range (50-1700 m/z), 1 scan per second. For E. coli, direct injection of 148-2960 cells in an injection volume of 10 μl generated satisfactory signal, without saturating the chip or MS.

Heinemann J., Noon B., Mohigmi M.J., Mazurie A., Dickensheets D.L, & Bothner B. (2014). Real-time digitization of metabolomics patterns from a living system using mass spectrometry. Journal of the American Society for Mass Spectrometry, 25(10), 1755-1762.

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Serum Biomarkers for Bone Health

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Fasting morning serum was collected from all subjects. Serum was
archived at −80 degrees C and analyzed in one batch after all visits were
completed. Laboratory assays were performed in the Core Laboratory of the CUMC
Clinical and Translational Research Center. Serum calcium, albumin, and
creatinine were measured using automated techniques. Serum 25-hydroxyvitamin
D₂ and D₃ were measured by Ultra-performance Liquid
Chromatography combined with tandem mass spectrometry (UPLC-MS/MS) using a 1290
UPLC and a 6410 Tandem Mass Spectrometer (Agilent, Santa Clara, CA). Inter-assay
coefficient of variation (CV) was 2.9% for 25OHD₂ and 5.4% for
25OHD₃. Intact parathyroid hormone (iPTH) was measured by
chemiluminescent immunoassay (CLIA, Siemens Healthcare Diagnostics, Deerfield,
IL; CV 8.3%). Serum C-terminal telopeptide of type 1 collagen (CTX) was measured
by ELISA (Immunodiagnostics Systems, Scottsdale AZ; CV <10%). Serum
osteocalcin was measured by ELISA (Immunodiagnostic Systems, Scottsdale,
Arizona; CV 2.7%). Serum bone alkaline phosphatase was measured by ELISA (Quidel
Corp, Sand Diego, CA; CV 7.6%).

Dash A.S., Agarwal S., McMahon D.J., Cosman F., Nieves J., Bucovsky M., Guo X.E., Shane E, & Stein E.M. (2019). Abnormal Microarchitecture and Stiffness in Postmenopausal Women with Isolated Osteoporosis at the 1/3 Radius. Bone, 132, 115211.

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Characterization of Radiolabeled Compounds

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All chemical reagents and solvents were purchased from commercial sources (Sigma-Aldrich, St. Louis, MO, USA; BroadPharm, San Diego, CA, USA; Fisher Scientific, Hampton, NH, USA) and used as received unless otherwise stated. For aqueous buffer solution preparation, Milli-Q water was obtained from a Millipore Gradient Milli-Q water system (Burlington, MA, USA). Nuclear magnetic resonance (NMR) spectra were recorded on a Bruker 400 MHz NMR (Billerica, MA, USA). Liquid Chromatography-Mass Spectrometry (LC-MS) of compounds were performed by an Agilent 6540 Accurate-Mass Quadrupole Time-of-Flight LC/MS system equipped with 1290 UPLC (Santa Clara, CA, USA). HPLC purifications were performed in an Agilent 1260 Infinity Preparative HPLC system equipped with 1260 photodiode array detector (PDA) and an Agilent Prep-C18 column (150 × 21.2 mm, 5 μm) (Santa Clara, CA, USA). The radiolabeled compounds were characterized by a Waters 600 HPLC system equipped with a Waters 2996 PDA (Milford, MA, USA) and an in-line Shell Jr. 2000 radio detector (Spotsylvania, VA, USA).

Debnath S., Hao G., Guan B., Thapa P., Hao J., Hammers H, & Sun X. (2022). Theranostic Small-Molecule Prodrug Conjugates for Targeted Delivery and Controlled Release of Toll-like Receptor 7 Agonists. International Journal of Molecular Sciences, 23(13), 7160.

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