Chloramphenicol cm

The bacterial strains and plasmids used in this study are listed in the Supplemental Material Table S2. Unless specified otherwise, S. aureus strains were cultivated in tryptic soy broth (TSB; Oxoid, Basingstoke, UK) medium with shaking at 200 rpm or on tryptic soy agar (TSA) at 37 °C. When required, the antibiotic of chloramphenicol (Cm) (Sangon Biotech, Shanghai, China) was added to the S. aureus cultures at 10 μg/mL for plasmid selection and maintenance.

The bacterial strains and plasmids used in this study are listed in Table 1. The APEC XM strain (O2:K1) was donated by Dr. Chengping Lu, Nanjing Agricultural University. It was isolated from a duck brain with symptoms of septicemia and meningitis. The clbA deletion mutant and complemented mutant were derived from the APEC XM. All bacteria were grown aerobically on Luria-Bertani (LB) plates or in LB broth at 37°C with agitation (180 rpm), except for the mutants containing the temperature-sensitive plasmid pCP20 or pKD46, which was grown at 30°C. Strains harboring antibiotic resistance genes were cultured in LB containing ampicillin (Amp, 100 μg/mL) (Sangon Biotech, Shanghai, China) or chloramphenicol (Cm, 34 μg/mL) (Sangon Biotech, Shanghai, China) when appropriate. Plasmids pKD3, pKD46 and pCP20 were used for the λ-Red mediated recombination system. pBR322 was used for the construction of the complemented mutant. To determine growth rates, bacteria were incubated at 37°C in LB broth for 24 h with continuous agitation (180 rpm). The number of live bacteria was measured at 1 h intervals by determining the optical density (OD) at 600 nm. The growth curve experiment was performed with three biological replicates.

The bacterial strains and plasmids used in this study are described in supplementary Table S4. Unless otherwise indicated, all P. aeruginosa strains were isogenic of wild‐type strain PAO1 and grown at 37°C in Luria broth (LB; Sangon), 1/10 LB (vol/vol) or ABTGC media, a chemically defined media
³⁵ (link). E. coli strains were grown at 37°C in LB. Antibiotics were added to the appropriate media at the following concentrations: for P. aeruginosa, gentamycin (Gm; Sangon) 30 µg/ml, carbenicillin (Carb, Sangon) 200 µg/ml; for E. coli, Gm 30 µg/ml, ampicillin (Ap; Sangon) 100 µg/ml, chloramphenicol (Cm, Sangon) 6 µg/ml, kanamycin (Kan; Sangon) 50 µg/ml. l‐Arabinose (MilliporeSigma) was added into the medium when strains contain the l‐arabinose inducible promoter P_BAD.