Cell culture reagents: FBS (cat. no. SH30087.01; Hyclone; GE Healthcare Life Sciences); DMEM-high glucose culture medium (50 mM; cat. no. 11965-092; Gibco; Thermo Fisher Scientific, Inc.); DMEM-low glucose culture medium (5 mM; cat. no. 10567-014; Gibco; Thermo Fisher Scientific, Inc.). H9C2 cells were rat embryonic cardiomyocytes purchased from the Cell Resource Center of Shanghai Academy of Life Sciences, Chinese Academy of Sciences; Norvasc was obtained from Sigma-Aldrich (Merck KGaA); ELISA assay kit of CaN (cat. no. E-EL-R0134c) was purchased from Elabscience Biotechnology Co., Ltd.; Fluo-3 AM (calcium ion fluorescent probe; cat. no. S1056) was from Beyotime Institute of Biotechnology; DNase I (RNase-free) was purchased from Beijing Transgen Biotech Co., Ltd.; reverse transcription kit and real-time PCR kit were obtained from Vazyme; CnAβ, nuclear factor of activated T cells 3 (NFAT3), β type myosin heavy chain (β-MHC) and b-actin primary antibodies (cat. nos. ab3673, ab66781, ab207926 and ab8227, respectively) were all purchased from Abcam.
Ab207926
Manufactured by Abcam
Sourced in United Kingdom
Ab207926 is a laboratory equipment product manufactured by Abcam. It serves as a core tool for various research applications. The product's primary function is to facilitate essential laboratory processes, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab8227, by Abcam (2 mentions)
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Ab3673, by Abcam (1 mentions)
Ab140631, by Abcam (1 mentions)
Ab133627, by Abcam (1 mentions)
Ab11575, by Abcam (1 mentions)
T0694, by Sangon (1 mentions)
Bca protein assay kit 23225, by Thermo Fisher Scientific (1 mentions)
4 protocols using ab207926
1
Cardiomyocyte Culture and Signaling Assays
2
Protein Expression Analysis in Cell Lysates
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Proteins extracted from cell lysates or supernatant culture medium were subjected to electrophoresis. After proteins were transferred and blocked, strips were subjected to rabbit anti-β-actin (1:1000; ab8227, Abcam, Cambridge, UK), anti-HOXA9 (1:1000; EPR3655(2) ab140631), anti-MyHC (1:1000; ab207926), anti-MyoD (1:1000; ab133627) antibodies overnight at 4° C.
3
Differentiated Myotube Analysis in C2C12 Cells
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C2C12 cells were fixed using 4% formaldehyde for 15–30 min, then treated with 0.1% Triton X‐100 (Sangon Biotech, T0694) for 10 min permeabilization. The samples were analysed using anti‐MyHC (Abcam, ab207926), and the cells nuclei were stained with DAPI. The differentiated myotube index was calculated as the percentage of the total image number covered by differentiated myotubes, and the measurement was performed using ImageJ software. TA muscle sections were prepared using anti‐Laminin (Abcam, ab11575) and anti‐embryonic myosin heavy chain (MYH3, Abcam, F1.652).
2 (link)
2 (link)
4
Phosphorylation of Cardiac Myosin Binding Protein-C in Myocardial Infarction
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The phosphorylation level of the cMyBP-C M-domain at three serine residues (p273, p282, p302) was determined in SD rats subjected to MI surgery using the Western Immunoassay (Wes) procedure (ProteinSimple, Santa Clara, CA). Left ventricle infarct tissue was lysed in a RIPA buffer (Thermo Fisher Scientific, Waltham, MA) supplemented with protease and phosphatase inhibitors. Protein estimation was performed as per kit (Pierce™ BCA Protein Assay Kit 23225, Thermo Fisher Scientific). Samples were prepared as per the Wes protocol, according to ProteinSimple recommendations. Antibodies used for the expression study are as follows: Anti-cMyBP-C p273 (1:250), cMyBP-C p-282 (1:250), cMyBP-C p302 (1:250) (all provided through material transfer agreement from Professor Sakthivel Sadayappan, College of Medicine, University of Cincinnati); cMyBP-C total (Santa Cruz Cat # SC-137182, Lot # E2913 [1:5 for Wes]); myosin heavy chain (Abcam ab207926 (3-48G5C7); alpha (cardiac) actin (Sigma-Aldrich A9357 clone AC1-20.4.2); Troponin I (Cell Signaling 13083S [D6F8]); and Troponin T (Sigma-Aldrich T6277 Clone JLT-12). Data were normalized to expressions of housekeeping proteins α-actin or hypoxanthine phosphoribosyl transferase (HPRT) to match the protein amount loaded.
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