Trypticase soya agar (TSA), Luria–Bertani (LB), Dulbecco’s modified Eagle’s medium (DMEM) fetal bovine serum (FBS), L-glutamine, penicillin, streptomycin, catalase kit, and MTT (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide) kit were obtained from Sigma-Aldrich (Madrid, Spain). Escherichia coli (E. coli CECT 516), Staphylococcus aureus (S. aureus CECT 8753), Enterococcus faecalis (E. faecalis CECT 184), Pseudomonas aeruginosa (P. aeruginosa CECT 108), and Salmonella typhimurium (S. thyphimurium CECT 409) were obtained from Colección Española de Cultivos (Spain). API 20E was obtained from BioMerieux España S.A. The HeLa cell line was purchased from CLS (Cell Line Service, Eppelheim, Germany). The UltraClean microbial DNA isolation kit was obtained from MO BIO Labs., Inc. (Solana Beach, CA, USA), and DNA purification JetQuick kit from Genomed (Leesburg, VA, USA).
Catalase kit
Manufactured by Merck Group
Sourced in Spain
The Catalase kit is a laboratory product designed to detect and measure the presence of the enzyme catalase in biological samples. Catalase is an important antioxidant enzyme found in most living organisms. The kit provides the necessary reagents and protocols to perform catalase activity assays in a simple and efficient manner.
Automatically generated - may contain errors
Lab products found in correlation
Trypticase soya agar, by Merck Group (2 mentions)
Jetquick dna purification kit, by Genomed (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions)
Penicillin, by Merck Group (2 mentions)
Streptomycin, by Merck Group (2 mentions)
L glutamine, by Merck Group (2 mentions)
Api 20e, by bioMérieux (2 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Thermo Fisher Scientific (1 mentions)
Fitc annexin 5 apoptosis detection kit, by BD (1 mentions)
Dmem medium, by Merck Group (1 mentions)
Bapta am, by Bio-Techne (1 mentions)
3 protocols using catalase kit
1
Bacterial Growth and Cytotoxicity Assays
2
Bacterial Growth and Cytotoxicity Assays
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Trypticase soya agar (TSA), Luria–Bertani (LB), Dulbecco’s modified Eagle’s medium (DMEM) fetal bovine serum (FBS), L-glutamine, penicillin, streptomycin, catalase kit, and MTT (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide) kit were obtained from Sigma-Aldrich (Madrid, Spain). Escherichia coli (E. coli CECT 516), Staphylococcus aureus (S. aureus CECT 8753), Enterococcus faecalis (E. faecalis CECT 184), Pseudomonas aeruginosa (P. aeruginosa CECT 108), and Salmonella typhimurium (S. thyphimurium CECT 409) were obtained from Colección Española de Cultivos (Spain). API 20E was obtained from BioMerieux España S.A. The HeLa cell line was purchased from CLS (Cell Line Service, Eppelheim, Germany). The UltraClean microbial DNA isolation kit was obtained from MO BIO Labs., Inc. (Solana Beach, CA, USA), and DNA purification JetQuick kit from Genomed (Leesburg, VA, USA).
3
Isolation and Purification of LAAO from B. leucurus Venom
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LAAO from B. leucurus venom was isolated and purified as described by Torres et al. (2010) [9 ]. DMEM medium, phenylhydrazone (FCCP), 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) and catalase kit were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Tetramethylrhodamine ethyl ester (TMRE), Fura-2AM and 2,7dichlorodihydrofluorescein diacetate (DCFH-DA) were purchased from Molecular Probes (Eugene, OR, USA). AnnexinV/FITC Apoptosis Detection Kit was from BD Pharmigen (CA, USA). Z-Val-Ala-Asp (OMe)-fluoromethylketone (zVAD), catalase and BAPTA-AM were from Tocris (Bristol, UK).
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