Log In Sign up
The largest database of trusted experimental protocols

Bx60 compound light microscope

Manufactured by Olympus
Visit Supplier

The BX60 is a compound light microscope manufactured by Olympus. It is designed for basic observation and analysis of biological and materials samples. The BX60 features a sturdy optical system and various objectives to provide clear and detailed images of specimens.

Automatically generated - may contain errors

Lab products found in correlation

3 3 diaminobenzidine (dab), by Vector Laboratories (2 mentions) Zn 5 antibody, by Vector Laboratories (2 mentions) N phenylthiourea, by Merck Group (2 mentions) Dig rna labeling mix, by Roche (2 mentions)

Close spelling variants of this product

BX60 microscope (531 citations) BX60 light microscope (96 citations) Compound microscope (22 citations) Compound light microscope (10 citations) BX60 compound microscope (6 citations) BX60 light (2 citations)

4 protocols using bx60 compound light microscope

1

Optic Nerve Quantification in Larvae

Check if the same lab product or an alternative is used in the 5 most similar protocols
Morpholino injected larvae were fixed at 96 hpf in 4% PFA overnight and then decapitated. Zn-5 antibody (ZFIN) was used at 1:200 followed by incubation with 1:200 HRP-conjugated secondary and finally developed with 3,3′-Diaminobenzidine DAB (Vector). After DAB development fish were depigmented with H2O2 and KOH, post fixed and dehydrated in ethanol. Samples were embedded in freeing media, sliced into 30μm sections, and imaged with an Olympus BX60 compound light microscope. 8-bit grey scale numbers were calculated from TIF files of sections contained the optic nerve using Fiji image analysis such that 0=white and 256=black and was converted to percentage whereby 0=white 100=black. 5 evenly spaced pixels from the inner plexiform layer were measured to find the average intensity per eye.
Abrams A.J., Hufnagel R.B., Rebelo A., Zanna C., Patel N., Gonzalez M.A., Campeanu I.J., Griffin L.B., Groenewald S., Strickland A.V., Tao F., Speziani F., Abreu L., Schüle R., Caporali L., La Morgia C., Maresca A., Liguori R., Lodi R., Ahmed Z.M., Sund K.L., Wang X., Krueger L.A., Peng Y., Prada C.E., Prows C.A., Bove K., Schorry E.K., Antonellis A., Zimmerman H.H., Abdul-Rahman O.A., Yang Y., Downes S.M., Prince J., Fontanesi F., Barrientos A., Nemeth A.H., Carelli V., Huang T., Zuchner S, & Dallman J.E. (2015). Mutations in the UGO1-like protein SLC25A46 cause an optic atrophy spectrum disorder. Nature genetics, 47(8), 926-932.
+ Open protocol
+ Expand
2

Optic Nerve Quantification in Larvae

Check if the same lab product or an alternative is used in the 5 most similar protocols
Morpholino injected larvae were fixed at 96 hpf in 4% PFA overnight and then decapitated. Zn-5 antibody (ZFIN) was used at 1:200 followed by incubation with 1:200 HRP-conjugated secondary and finally developed with 3,3′-Diaminobenzidine DAB (Vector). After DAB development fish were depigmented with H2O2 and KOH, post fixed and dehydrated in ethanol. Samples were embedded in freeing media, sliced into 30μm sections, and imaged with an Olympus BX60 compound light microscope. 8-bit grey scale numbers were calculated from TIF files of sections contained the optic nerve using Fiji image analysis such that 0=white and 256=black and was converted to percentage whereby 0=white 100=black. 5 evenly spaced pixels from the inner plexiform layer were measured to find the average intensity per eye.
Abrams A.J., Hufnagel R.B., Rebelo A., Zanna C., Patel N., Gonzalez M.A., Campeanu I.J., Griffin L.B., Groenewald S., Strickland A.V., Tao F., Speziani F., Abreu L., Schüle R., Caporali L., La Morgia C., Maresca A., Liguori R., Lodi R., Ahmed Z.M., Sund K.L., Wang X., Krueger L.A., Peng Y., Prada C.E., Prows C.A., Bove K., Schorry E.K., Antonellis A., Zimmerman H.H., Abdul-Rahman O.A., Yang Y., Downes S.M., Prince J., Fontanesi F., Barrientos A., Nemeth A.H., Carelli V., Huang T., Zuchner S, & Dallman J.E. (2015). Mutations in the UGO1-like protein SLC25A46 cause an optic atrophy spectrum disorder. Nature genetics, 47(8), 926-932.
+ Open protocol
+ Expand
3

RNA in situ hybridization in zebrafish

Check if the same lab product or an alternative is used in the 5 most similar protocols
For RNA in situ hybridization, wild-type embryos were incubated in n-phenylthiourea (Sigma) to suppress pigment development. The slc25a46 probe sequence was PCR amplified from zebrafish cDNA (Supplementary Table 5) and the DIG RNA Labeling Mix (Roche) was used to synthesize the antisense probe. Probe hybridization and development were carried out using standard protocol39 (link), followed by post fixation in 4% PFA and dehydration in 30% sucrose. Embryos were embedded in tissue freezing media (Triangle Biomedical Sciences), sliced with a cryostat into 30μm sections, and imaged with an Olympus BX60 compound light microscope.
Abrams A.J., Hufnagel R.B., Rebelo A., Zanna C., Patel N., Gonzalez M.A., Campeanu I.J., Griffin L.B., Groenewald S., Strickland A.V., Tao F., Speziani F., Abreu L., Schüle R., Caporali L., La Morgia C., Maresca A., Liguori R., Lodi R., Ahmed Z.M., Sund K.L., Wang X., Krueger L.A., Peng Y., Prada C.E., Prows C.A., Bove K., Schorry E.K., Antonellis A., Zimmerman H.H., Abdul-Rahman O.A., Yang Y., Downes S.M., Prince J., Fontanesi F., Barrientos A., Nemeth A.H., Carelli V., Huang T., Zuchner S, & Dallman J.E. (2015). Mutations in the UGO1-like protein SLC25A46 cause an optic atrophy spectrum disorder. Nature genetics, 47(8), 926-932.
+ Open protocol
+ Expand
4

RNA in situ hybridization in zebrafish

Check if the same lab product or an alternative is used in the 5 most similar protocols
For RNA in situ hybridization, wild-type embryos were incubated in n-phenylthiourea (Sigma) to suppress pigment development. The slc25a46 probe sequence was PCR amplified from zebrafish cDNA (Supplementary Table 5) and the DIG RNA Labeling Mix (Roche) was used to synthesize the antisense probe. Probe hybridization and development were carried out using standard protocol39 (link), followed by post fixation in 4% PFA and dehydration in 30% sucrose. Embryos were embedded in tissue freezing media (Triangle Biomedical Sciences), sliced with a cryostat into 30μm sections, and imaged with an Olympus BX60 compound light microscope.
Abrams A.J., Hufnagel R.B., Rebelo A., Zanna C., Patel N., Gonzalez M.A., Campeanu I.J., Griffin L.B., Groenewald S., Strickland A.V., Tao F., Speziani F., Abreu L., Schüle R., Caporali L., La Morgia C., Maresca A., Liguori R., Lodi R., Ahmed Z.M., Sund K.L., Wang X., Krueger L.A., Peng Y., Prada C.E., Prows C.A., Bove K., Schorry E.K., Antonellis A., Zimmerman H.H., Abdul-Rahman O.A., Yang Y., Downes S.M., Prince J., Fontanesi F., Barrientos A., Nemeth A.H., Carelli V., Huang T., Zuchner S, & Dallman J.E. (2015). Mutations in the UGO1-like protein SLC25A46 cause an optic atrophy spectrum disorder. Nature genetics, 47(8), 926-932.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!