Chitosan oligosaccharides classified by molecular weight (GO2KA1; MW < 1000 Da) were purchased from Kunpoong Bio Co. Ltd. (Jeju, Korea). Corn starch, casein, vitamin mix, mineral mix, calcium phosphate and sodium chloride were purchased from Raon Bio (Yonginsi, Korea). Total cholesterol and total glyceride kits were purchased from Stanbio laboratory (Boerne, TX, USA). Unless noted, otherwise, all chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The fast SYBR real-time PCR master mix, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), bovine calf newborn serum (BCS), penicillin-streptomycin (P/S), and trypsin-EDTA were obtained from Life Technologies (Grand Island, NY, USA). Adiponectin ELISA kit was purchased from Thermo Fisher Scientific (Invitrogen, Carlsbad, CA, USA). 3T3-L1 cells (ATCCV®CL-173TM) were used below passage 12. 3T3-L1 preadipocytes were propagated and cultured in DMEM medium supplemented with 10% BCS and 1% P/S until confluent and maintained for additional 2 days and differentiated as reported previously [26 (link)] with or without GO2KA1.
Adiponectin elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Adiponectin ELISA kit is a quantitative immunoassay designed for the measurement of adiponectin levels in biological samples. It uses the enzyme-linked immunosorbent assay (ELISA) principle to detect and quantify the target analyte.
Automatically generated - may contain errors
Lab products found in correlation
Total cholesterol and total glyceride kits, by EKF Diagnostics (2 mentions)
Fast sybr real time pcr master mix, by Thermo Fisher Scientific (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Tnf α elisa kit, by Thermo Fisher Scientific (1 mentions)
Insulin elisa kit, by ALPCO (1 mentions)
3 protocols using adiponectin elisa kit
1
Chitosan Oligosaccharides Modulate 3T3-L1 Adipogenesis
2
Arginyl-fructose Enriched Barley Powder
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Arginyl-fructose (AF)-enriched barley powder (BEE) was purchased from Kunpoong Bio Co., Ltd. (Jeju, Korea). Corn starch, casein, vitamin mix, mineral mix, calcium phosphate, and sodium chloride were purchased from Raon Bio (Yonginsi, Korea). Total cholesterol and total glyceride kits were purchased from Stanbio laboratory (Boerne, TX, USA). Unless noted, all chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The fast SYBR real-time PCR master mix, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), bovine calf newborn serum (BCS), penicillin-streptomycin (p/S), and trypsin-EDTA were obtained from Life Technologies (Grand Island, NY, USA). Adiponectin ELISA kit was purchased from Thermo Fisher Scientific (Invitrogen, Carlsbad, CA, USA). 3T3-L1 cells (ATCCV®CL-173TM) were used below passage 12. 3T3-L1 preadipocytes were propagated and cultured in DMEM medium supplemented with 10% BCS and 1% P/S until confluent and maintained for additional 2 days and differentiated as reported previously [26 (link)] with or without BEE.
3
Metabolic Biomarkers and Glucose Homeostasis
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The plasma levels of glucose, insulin, TNFα, leptin and adiponectin and hepatic AMPK activity, were measured using a glucose meter (OneTouch, LifeScan, Milpitas, CA, USA), Insulin ELISA kit (ALPCO, Salem, NH, USA), TNFα ELISA kit (Invitrogen), Leptin ELISA kit (Novex), Adiponectin ELISA kit (Thermo Fisher Scientific) and AMPK kinase assay kit (Cyclex, Nagano, Japan), respectively, according to the manufacturers' recommended protocols.
For the glucose tolerance test, the mice were subjected to fasting for 16 h, and then, injected intraperitoneally with glucose (1 g/kg body weight). For the insulin tolerance test, insulin was injected intraperitoneally at 0.75 U/kg body wt. The blood glucose level was measured via tail bleeding.
For the glucose tolerance test, the mice were subjected to fasting for 16 h, and then, injected intraperitoneally with glucose (1 g/kg body weight). For the insulin tolerance test, insulin was injected intraperitoneally at 0.75 U/kg body wt. The blood glucose level was measured via tail bleeding.
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