Npf gal4

Manufactured by Bloomington Drosophila Stock Center

The Npf-GAL4 is a genetic tool used to manipulate the expression of target genes in Drosophila (fruit flies). It functions by driving the expression of the GAL4 transcriptional activator under the control of the Neuropeptide F (Npf) promoter, allowing for the selective targeting of Npf-expressing cells or tissues.

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7 protocols using npf gal4

Drosophila Circadian Rhythm Genetics

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Flies were grown on standard cornmeal-molasses medium and maintained at 25°C. The following strains were used: Iso31 (isogenic w1118 stock; (Ryder et al., 2004 (link))), Pdf-GAL4 (Renn et al., 1999 (link)), 911-GAL4 (InSITE Library; (del Valle Rodríguez et al., 2012 (link))), Dvpdf-GAL4; pdf-GAL80 (Guo et al., 2014 (link)), Clk^jrk (Allada et al., 1998 (link)), and UAS-npf RNAi (Vienna Drosophila Resource Center #108772). The following flies were obtained from Bloomington Drosophila Stock Center: Npf-GAL4 (#25681), UAS-CLKΔ (#36318), UAS-CYCΔ (#36317), tub-GAL80^ts (#7018), and npfr mutant (#10747).

Erion R., King A.N., Wu G., Hogenesch J.B, & Sehgal A. (2016). Neural clocks and Neuropeptide F/Y regulate circadian gene expression in a peripheral metabolic tissue. eLife, 5, e13552.

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Fly Behavioral Assay Protocols

Cited 2 times

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Flies were raised at 25°C and 60% relative humidity on standard cornmeal fly food under 12:12h light:dark cycle. 4–7 days old flies were used for experiments and were transferred to fresh food vials 48h before behavioral tests. Fly population was randomized but was kept age matched in each trial. For food deprivation, flies were kept in empty bottles with a wet cotton plug to prevent desiccation. The following fly lines were ordered from Bloomington stock center: Npf-Gal4 (25681), npfr mutants (10747), 20XUAS-TTS-shi[ts1]-p10 (66600; referred to as UAS-shibire^ts1 in the text), UAS-TrpA1 (26263), MB461B (68327), MB370B (68319), MB504B (68329), MB296B (68308), MB320C (68253), MB077B (68283) and MB112C (68263). UAS-npf-RNAi (108772), UAS-npfr-RNAi (107663) and VT50658 (200166) were ordered from the Vienna Drosophila Resource Center. Other fly lines were described previously: redeye^{6 (link)}, R26E01^{15 (link)}, R35B12¹⁶, and UAS-CaLexA^{17 (link)}. The background control line was Canton-S (Heisenberg) strain.

Chouhan N.S., Griffith L.C., Haynes P, & Sehgal A. (2020). Availability of food determines the need for sleep in memory consolidation. Nature, 589(7843), 582-585.

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Fly Behavioral Assay Protocols

Cited 2 times

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Chouhan N.S., Griffith L.C., Haynes P, & Sehgal A. (2020). Availability of food determines the need for sleep in memory consolidation. Nature, 589(7843), 582-585.

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Drosophila Rearing and Genetics Procedures

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Drosophila melanogaster flies were raised on standard medium (water, cornmeal, soy bean, agar, molasses, yeast, preservative methyl-4-hydroxybenzoate) at 25 °C, 60% humidity and 14 h/10 h light–dark cycles. Canton-S (CS) served as wild-type Drosophila strain in this study. The following colleagues kindly provided additional stocks: D. Armstrong (189Y-GAL4), M. Heisenberg (wild-type Canton-S, rut¹, and dnc¹), T.J. Hirsh (Trh493-GAL4), Y. Rao (5HT-1A-GAL4), H. Scholz (UAS-TNT), P. Shen (UAS-npf), H. Tanimoto (H24-GAL4, NP1131-GAL4 and mb247-GAL4; Tub-GAL80^ts), and T. Zars (mb247-GAL4). The following lines were obtained from the Bloomington Stock Center: Or83b-GAL4 (#2681), c305a-GAL4 (#30829), c739-GAL4(#7362), npf-GAL4 (#25681), 5HT-1B-GAL4 (#27637), UAS-NaChBac (#9469), UAS-5HT-1A^RNAi (#25834 and #33885), UAS-5HT-1B^RNAi (#27634 and #54006), UAS-CD4::tdTomato (#35837) and UAS-mCD8::GFP (#5130).

Ries A.S., Hermanns T., Poeck B, & Strauss R. (2017). Serotonin modulates a depression-like state in Drosophila responsive to lithium treatment. Nature Communications, 8, 15738.

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Transgenic Fly Behavioral Assays

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All flies are in the w¹¹¹⁸ background. Larvae were reared at 25 °C, and early third instars (~74 hr after egg laying, AEL) were fed before behavioral experiments as previously described^{7 (link)}. The transgenic flies include TH-GAL4^{16 (link)}, UAS-dTrpA1^{31 (link)}, UAS-GCaMP3 (BL32116), UAS-GCaMP6(BL42749), UAS-TH^RNAi (BL25796), and npf-GAL4 were obtained from the Bloomington Stock Center. UAS-Dop1R1^RNAi (V107058) was obtained from the Vienna Drosophila RNAi Center. DopR^f02676 flies were described previously^{18 (link)}.

Pu Y., Palombo M.M, & Shen P. (2018). Contribution of DA Signaling to Appetitive Odor Perception in a Drosophila Model. Scientific Reports, 8, 5978.

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Diverse Drosophila Transgenic Lines

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The following lines were obtained from the Bloomington Drosophila Stock Center: nSyb-GAL4 (#51635), vGlut-GAL4 (#26160), ChAT-GAL4 (#6793), vGAT-GAL4 (#58409), Trh-GAL4 (#38389), Tdc2-GAL4 (#9313), OK107-GAL4 (#854), Akh-GAL4 (#25683), Mip-GAL4 (#51983), Ilp2-GAL4 (#37516), Eth-GAL4 (#51982), Fmrfa-GAL4 (#56837), Lk-GAL4 (#51993), NPF-GAL4 (#25681), phm-GAL4 (#80577), ple-GAL4 (#8848), SifAR-GAL4 (#76670), Tk-GAL4 (#51973), Eip93F RNAi (#57868), EcR RNAi (#58286), vGAT RNAi (#41958), and tubP-GAL80ts (#7019). E93-RNAi, nSyb-GAL4, mCherry-RNAi lines were outcrossed for five generations to a Berlin background (originally from the Heisenberg lab). Flies were raised on standard cornmeal/molasses food and incubated at 25 °C under a 12 h light/12 h dark cycle.

Yip C., Wyler S.C., Liang K., Yamazaki S., Cobb T., Safdar M., Metai A., Merchant W., Wessells R., Rothenfluh A., Lee S., Elmquist J, & You Y.J. (2024). Neuronal E93 is required for adaptation to adult metabolism and behavior. Molecular Metabolism, 84, 101939.

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Drosophila Behavioral Protocols in w^1118 Background

Cited 1 time

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All flies are in the w¹¹¹⁸ background. Larvae were reared at 25 °C, and early third instars (~74 hr after egg laying, AEL) were fed before behavioral experiments as previously described^{7 (link)}. The transgenic flies including TH-GAL4^{23 (link)}, UAS-dTrpA1^{20 (link)},UAS-GCaMP6.0 s (BL42749), UAS-Arclight (BL51056), UAS-GPF.nls (BL4776) and npf-GAL4 were obtained from Bloomington stock center. UAS-Dop1R1^RNAi (V107058) was obtained from the Vienna Drosophila RNAi Center. UAS-CD4::spGFP^1–10, LexAop-CD4::spGFP¹¹ were kindly provided by K. Scott^{19 (link)}.

Pu Y., Zhang Y., Zhang Y, & Shen P. (2018). Two Drosophila Neuropeptide Y-like Neurons Define a Reward Module for Transforming Appetitive Odor Representations to Motivation. Scientific Reports, 8, 11658.

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