Image pro plus 6.0 ipp

Manufactured by Media Cybernetics

Sourced in United States

Image-Pro Plus 6.0 (IPP) is a comprehensive software package designed for image acquisition, processing, and analysis. It provides a suite of tools for tasks such as image enhancement, measurement, and quantification. The software is compatible with a wide range of imaging devices and supports various file formats.

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Lab products found in correlation

Anti claudin 1, by Abcam (1 mentions) Anti mucin 2, by Abcam (1 mentions) Anti lyz, by Abcam (1 mentions) Anti occludin, by Proteintech (1 mentions) Pannoramic digital slide scanner, by 3DHISTECH (1 mentions) Ab2349, by Abcam (1 mentions) Dm4000b, by Leica (1 mentions)

Close spelling variants of this product

Image-Pro Plus 6.0 (4438 citations) Image-Pro Plus (1608 citations) Image-Pro (129 citations) Image-Pro 6.0 (113 citations) IPP 6.0 software (43 citations) Image Pro Plus (IPP; (9 citations) Image-Pro Plus 6.0 image (7 citations) Image-Pro Plus 6.0 (IPP 6.0) (4 citations) Image Pro Plus 6 (IPP; (4 citations) Image Pro Plus 6.0 (IPP) software (2 citations)

5 protocols using image pro plus 6.0 ipp

Lung Histopathology and Immunohistochemical Analysis

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For histopathology examination, lung samples were fixed in 10% neutral buffered formalin, which was made with PBS (pH 7.4, 0.1 M), immediately after collection for histopathological and immunohistochemical examination. Paraffin-embedded sections (4 μm thick) were cut, and conventional hematoxylin and eosin (H&E) staining was performed for histopathological observation [17 (link)]. These sections were evaluated in a blinded fashion by a pathologist. For quantitative analysis of inflammatory cells lymphocytes, neutrophils, and monocytes, one slide for each lung was randomly selected, and 10 fields of each slide were evaluated for cell counting using light microscopy. The total cell amount for each group was then compared.
The existence of IL-6 and TNF-α in the lungs was determined after immunohistochemical staining with the IL-6 and TNF-α antibodies. One slide for each lung was selected randomly, and 5 fields of each slide were evaluated under light microscopy. A brown positive signal was quantitatively expressed as the integrated optical density (IOD), and the average IOD per field for individual lungs was calculated using Image-Pro Plus 6.0 (IPP6) software (Media Cybernetics, Inc., Bethesda, MD, USA). A comparison of the total IODs between each of the two groups was performed [20 (link)].

Wang J., Zhu X., Peng Y., Zhu T., Liu H., Zhu Y., Xiong X., Chen X., Hu C., Chen H., Chen Y, & Guo A. (2020). Mycobacterium tuberculosis YrbE3A Promotes Host Innate Immune Response by Targeting NF-κB/JNK Signaling. Microorganisms, 8(4), 584.

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Immunohistochemical Analysis of Ileum Tissue

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The 4% paraformaldehyde fixed ileum tissue samples were embedded in paraffin and sectioned in 5‐μm thickness. The following primary antibodies were incubated overnight at 4°C: anti‐mucin 2 (1:2000; Abcam, Cambridge, UK), anti‐defensin 5 (1:50; Abcam), anti‐defensin 6 (1:5000; Atlas, Bromma, Sweden), anti‐LYZ (1:100; Abcam), anti‐Occludin (1:100; Proteintech, Rosemont, IL, USA), anti‐zonula occludens 1 (anti‐ZO 1) (1:200; Abcam), and anti‐Claudin 1 (1:700; Abcam). The sections were subsequently incubated for 60 min with a biotinylated secondary antibody (1:100; Zhongshan, Beijing, China). All histological images were captured under a light microscope (Leica, Wetzlar, Germany) at 200× magnification. Image‐Pro Plus 6.0 (IPP6.0; Media Cybernetics, Inc., Rockville, MD, USA) was used for morphological analysis.
The inflammation score was determined by a blinded observer and was strictly based on a fully proven method that assigns a histological score of 0–4 to quantify the degree of inflammation. The criteria of this system include inflammatory cells, goblet cell depletion, immune infiltration, and architecture destruction.¹¹

Li X., Ma H., Sun Y., Li T., Wang C., Zheng H., Chen G., Du G., Ji G., Yang H., Xiao W, & Qiu Y. (2022). Effects of fecal stream deprivation on human intestinal barrier after loop ileostomy. Journal of Gastroenterology and Hepatology, 37(6), 1119-1130.

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Quantifying Myelin Levels in Mouse Brains

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Mouse brains were embedded and cut into 15‐μm sections, followed by deparaffinization. The sections were then rehydrated in a solution of LFB (0.01% LFB in 95% ethanol) and incubated overnight at 56°C. After washing in 95% and 75% ethanol and removing excess stain, the sections were differentiated in lithium carbonate solution for 15 s, followed by washing in distilled water. Subsequently, the sections were dehydrated and fixed. Images were scanned using PANNORAMIC Digital Slide Scanners (3DHISTECH Ltd, Hungary). Quantification of LFB staining was performed within the corpus callosum zone of three mice per group using the image analysis system Image‐Pro Plus 6.0 (IPP 6.0, Media Cybernetics, Bethesda, MD, USA). The LFB intensity was measured and normalized to the corresponding area to obtain the final calculated results.

Chen S., Liu S., Huang Y., Huang S., Zhang W., Xie H, & Lu L. (2024). 5Z‐7‐Oxozaenol attenuates cuprizone‐induced demyelination in mice through microglia polarization regulation. Brain and Behavior, 14(4), e3487.

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Immunohistochemical Quantification of Lon Protein

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The TMA were reviewed and scored independently by two pathologists who had no prior knowledge of the clinicopathological features of the specimens on the TMA. For IHC quantification, three random representative 400× microscopic fields per section were photographed using a standard Nikon Light Microscope. Then using imaging analysis software Image-Pro Plus 6.0 (IPP) (Media Cybernetics, Rockville, MD) for digital photographs analysis by calculation of integrated optical density (IOD) referencing the method introduced previously [42 (link)]. IOD, the integral calculus of the stained area times the intensity of staining in each pixel in the area, indicates the total amount of staining material in that area. Lon protein expression levels in the TMA were evaluated by scanning the entire tissue microarrays.
The IHC staining of Lon was assessed according to the immune-reactive score (IRS) as described previously [43 (link), 44 (link)], and adjusted slightly, which evaluated both the percentage of positive cells and the staining intensity. The percentage of positive cells was scored as 1 (≤10%), 2 (10-50%), 3 (50-80%), 4 (≥80%)[45 (link)]; staining intensity was graded as 0 (negative), 1 (weak), 2 (moderate), and 3 (strong). The two scores were multiplied and the IRS (values from 0 to 12) was determined as “Low” and “High” corresponding to IRS values of ≤ 6 and>6, respectively.

Liu Y., Lan L., Huang K., Wang R., Xu C., Shi Y., Wu X., Wu Z., Zhang J., Chen L., Wang L., Yu X., Zhu H, & Lu B. (2014). Inhibition of Lon blocks cell proliferation, enhances chemosensitivity by promoting apoptosis and decreases cellular bioenergetics of bladder cancer: potential roles of Lon as a prognostic marker and therapeutic target in baldder cancer. Oncotarget, 5(22), 11209-11224.

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Immunofluorescence Staining of Renal Sections

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Immunofluorescence staining of renal sections were conducted as previously described [8 (link)]. First, antigens were retrieved by boiling in citric acid buffer (pH 6.0) for 15 min. Nonspecific antigens were blocked with 5% bovine serum albumin for 1 h at room temperature. Samples were incubated with rabbit anti-VEGFR2 antibody (ab2349, 1 : 500, Abcam, Cambridge, UK) at 4°C overnight, and then goat anti-rabbit DyLight 488 Antibody (1031-02, 1 : 20,000, Vector Laboratories, Burlingame, CA, USA) or anti-rabbit CY3 antibody (CY-1300-1, 1 : 2000, Vector Laboratories, Burlingame, CA, USA) was added for 1 h. Subsequently, DAPI was applied for 8 min. Stained sections were imaged at 200x on a fluorescence microscope (Leica DM4000B, Leica Microsystems, Wetzlar, Germany), and quantitative analysis was performed using Image-Pro Plus 6.0 (IPP; Media Cybernetics, Rockville, MD, USA) [21 (link)].

Zheng G.H., Shan Q., Mu J.J., Wang Y.J., Zhang Z.F., Fan S.H., Hu B., Li M.Q., Xie J., Chen P., Wu D.M., Lu J, & Zheng Y.L. (2019). Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice. Oxidative Medicine and Cellular Longevity, 2019, 5189819.

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