Efficient navigation 2

Fluorescent images were obtained at RT with an AxioImager.M2 microscope (Carl Zeiss, Oberkochen, Germany) with an ApoTome2 grid confocal unit (Carl Zeiss) using EC Plan-Neofluar 40×/0.75-NA Air (Carl Zeiss) or Plan-Apochromat 40×/0.95-NA Air (Carl Zeiss) objectives for fat cells, and Plan-Apochromat 63×/1.40-NA Oil (Carl Zeiss) objective for nephrocytes, an Orca Flash 4.0 LT sCMOS camera (Hamamatsu Photonics, Hamamatsu, Japan), and Zeiss Efficient Navigation 2 software (Carl Zeiss). Immersol 518F (Carl Zeiss) immersion oil was used for the 63x objective. Images from 8 consecutive focal planes (section thickness: 0.25 µm for nephrocytes and 0.35 µm for fat cells) were projected onto one single image, except for the colocalization assays, where we aimed to exclude any false positive colocalization, thus assessing only one focal plane. Images were processed in Zeiss Efficient Navigation 2 (Carl Zeiss) and Photoshop CS4 or CS6 (Adobe, San Jose, CA, USA) to present final figures.

Fluorescent images were obtained at RT with an AxioImager.M2 microscope (Carl Zeiss, Oberkochen, Germany) with an ApoTome2 grid confocal unit (Carl Zeiss) using EC Plan-Neofluar 40×/0.75-NA Air (Carl Zeiss) or Plan-Apochromat 40×/0.95-NA Air (Carl Zeiss) objectives for fat cells, and Plan-Apochromat 63×/1.40-NA Oil (Carl Zeiss) objective for nephrocytes, an Orca Flash 4.0 LT sCMOS camera (Hamamatsu Photonics, Hamamatsu, Japan), and ZEISS Efficient Navigation 2 software (Carl Zeiss). Immersol 518F (Carl Zeiss) immersion oil was used for the 63× objective. Images from 8 consecutive focal planes (section thickness: 0.25 µm for nephrocytes and 0.35 µm for fat cells) were projected onto one single image, except for the colocalization assays, where we aimed to exclude any false positive colocalization. Salivary gland images were taken using an AxioImager Z1 microscope (Carl Zeiss) at RT equipped with an Apotome1 grid confocal unit and an AxioCam MRm camera and an EC Plan-Neofluar 40×/0.75-NA objective and AxioVision SE64 Rel. 4.9.1 (Carl Zeiss) software. Images were processed in ZEISS Efficient Navigation 2 (Carl Zeiss) and Photoshop CS4 or CS6 (Adobe, San Jose, CA, USA) to present final figures. Compound eye pictures were taken using a Lumar V12 stereomicroscope (Carl Zeiss) equipped with AxioCam ERc5s camera (Carl Zeiss).