Umr 1149

On day 90, blood was collected from mice before euthanasia by submandibular puncture into tubes containing 5 µL EDTA 0.5 mol/L. After centrifugation (6000× g, 20 min, 4 °C), plasma was aliquoted and frozen at −80 °C until further analysis. Measurements of plasma AST, ALT, triglycerides (TG), high-density lipoprotein cholesterol (HDL), total cholesterol and ferritin were performed on the biochemistry platform (CRI, UMR 1149, Paris) with an Olympus AU400 Chemistry Analyzer. LDL (low-density lipoprotein) cholesterol was calculated according to the Friedewald formula:
Measurements of non-fasting plasma insulin and leptin were performed using a mouse-specific insulin and leptin ELISA Kit (Merck Millipore, Burlington, MA, USA).

On day 90, blood was collected from mice before euthanasia by submandibular puncture into tubes containing 5 µL EDTA 0.5 mol/L. After centrifugation (6000× g, 20 min, 4 °C) plasma was aliquoted and frozen at −80 °C until further analysis. Measurements of plasma aspartate-transferase (AST), alanine-transaminase (ALT), triglycerides (TG), high-density lipoprotein cholesterol (HDL), total cholesterol, and ferritin were performed using the biochemistry platform (CRI, UMR 1149, Paris) with an Olympus AU400 Chemistry Analyzer. Llow-density lipoprotein (LDL) cholesterol was calculated according to the Friedewald formula: LDL-cholesterol = total cholesterol − HDL-cholesterol − TG/2.2 (mmol/L), with all TG < 4.6 mmol/L.
Measurements of non-fasting plasma insulin and leptin were performed using mouse-specific insulin and leptin ELISA Kit (Merck Millipore, Molsheim, France).

Blood samples were collected in heparin-coated tubes via cardiac puncture, centrifuged and then plasma samples were stored at −80 °C. Plasma cholesterol, triglycerides, high-density lipoprotein (HDL), aspartate transaminase (AST) and alanine transaminase (ALT) measurement were performed by the Biochemistry Platform (CRI, UMR 1149, Paris) using Olympus AU400 Chemistry Analyzer.