Mice were anesthetized with sodium pentobarbital (50 mg/kg, i.p.), and the abdominal arteries were isolated, gently cleaned of fat and connective tissue, and cut into rings (1 mm in length) as previously reported [7 (link),29 (link)]. Rings were suspended vertically between stainless steel hooks in organ baths (20 mL) with modified Krebs–Henseleit solution (room temperature) containing (in mM): NaCl 115, KCl 4.7, CaCl2 2.5, MgCl2 1.2, NaHCO3 25, KH2PO4 1.2, and dextrose 10 in order to record the tension. The changes in isometric tension were measured with a force–displacement transducer (TB-651T; Nihon Kohden, Tokyo, Japan) connected to a carrier amplifier (EF601G; Nihon Kohden) and recorded with a pen recorder (WT-645G; Nihon Kohden). The bathing medium was maintained at 37 °C and bubbled continuously with 95% air and 5% CO2. Arterial rings were washed and allowed to equilibrate for 30 min. To measure the tension when the aortic ring snapped, the isometric tension was gradually increased (n = 7, each group).
Tb 651t
Manufactured by Nihon Kohden
Sourced in Japan
The TB-651T is a compact and portable blood pressure monitor designed for professional use in medical settings. It provides accurate blood pressure and pulse rate measurements through the oscillometric method. The device features a large, easy-to-read digital display and operates on AC power or battery power for increased mobility. The TB-651T is intended for clinical applications where reliable blood pressure monitoring is required.
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Lab products found in correlation
3 protocols using tb 651t
1
Measuring Tension in Aortic Rings
2
Isometric Tension Measurement of Aortic Rings
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Mice were anesthetized with sodium pentobarbital (50 mg/kg, i.p.), and the abdominal arteries were isolated, gently cleaned of fat and connective tissue, and cut into rings (1 mm in length) as previously reported [16] (link). Rings were suspended vertically between stainless steel hooks in organ baths (20 ml) with modified Krebs–Henseleit solution (room temperature) containing (in mM): NaCl 115, KCl 4.7, CaCl2 2.5, MgCl2 1.2, NaHCO3 25, KH2PO4 1.2, and dextrose 10 in order to record the tension. The changes in isometric tension were measured with a force–displacement transducer (TB-651T; Nihon Kohden, Tokyo, Japan) connected to a carrier amplifier (EF601G; Nihon Kohden) and recorded with a pen recorder (WT-645G; Nihon Kohden). The bath medium was maintained at 37°C and bubbled continuously with 95% air and 5% CO2. Arterial rings were washed and allowed to equilibrate for 30 min. To measure the tension when the aortic ring snapped, the isometric tension was gradually increased (n = 12, each group).
3
Isometric Contraction and Heart Rate Measurements
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Isometric contraction and HR were measured as described previously [9 (link), 15 (link)]. Male Wistar rats (127–313
g; 5–9-week-old) were deeply anesthetized with urethane (1.5 g/kg, i.p.) and sacrificed.
The left and right atria were isolated for the measurement of isometric contraction and
HR, respectively. Left and right atria were placed in a 15-ml organ bath
filled with Krebs-Henseleit solution, which contained the following compositions (mM): 119
NaCl, 4.8 KCl, 1.2 KH2PO4, 1.2 MgSO4, 2.5
CaCl2, 24.9 NaHCO3, and 10 glucose. The solution was saturated
with a 95% O2–5% CO2 mixture at 37°C. Left atrium, which was
electrically stimulated via a pair of platinum electrodes (field stimulation, 1 Hz, 5
msec, and 1.5 times threshold voltage) connected to an electronic stimulator (SEN-3301;
Nihon Kohden, Tokyo, Japan), was equilibrated at least for 70 min. Right atrium, which was
not electrically stimulated, was also equilibrated at least for 70 min. The resting
tension was adjusted to 0.5 g. Isometric contraction and HR were measured and recorded
with a force-displacement transducer (TB-651T; Nihon Kohden) and PowerLab system.
g; 5–9-week-old) were deeply anesthetized with urethane (1.5 g/kg, i.p.) and sacrificed.
The left and right atria were isolated for the measurement of isometric contraction and
HR, respectively. Left and right atria were placed in a 15-ml organ bath
filled with Krebs-Henseleit solution, which contained the following compositions (mM): 119
NaCl, 4.8 KCl, 1.2 KH2PO4, 1.2 MgSO4, 2.5
CaCl2, 24.9 NaHCO3, and 10 glucose. The solution was saturated
with a 95% O2–5% CO2 mixture at 37°C. Left atrium, which was
electrically stimulated via a pair of platinum electrodes (field stimulation, 1 Hz, 5
msec, and 1.5 times threshold voltage) connected to an electronic stimulator (SEN-3301;
Nihon Kohden, Tokyo, Japan), was equilibrated at least for 70 min. Right atrium, which was
not electrically stimulated, was also equilibrated at least for 70 min. The resting
tension was adjusted to 0.5 g. Isometric contraction and HR were measured and recorded
with a force-displacement transducer (TB-651T; Nihon Kohden) and PowerLab system.
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