The NQO1 activity assays kit (#ab184867) was purchased from Abcam (Cambridge, MA, USA). According to the manufacturer’s instructions, OC3-IV2 cells were lysed with 1X extraction buffer containing 1 mM phenylmethylsulfonyl fluoride for 15 min on ice, and then centrifuged at 17,000 × g at 4 °C for 20 min. The supernatant was collected, and then, the protein concentration of each sample was determined by bicinchoninic acid assays (Santa Cruz Biotechnology). A diluted sample and reaction buffer were added into 96-wells plate containing menadione or isoplumbagin with cofactor NADH and water-soluble tetrazolium salt (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, a highly sensitive tetrazolium reagent). The absorbance of reduced water-soluble tetrazolium salt was measured at 450 nm wavelengths by a spectrophotometer.
Nqo1 activity assays kit
Manufactured by Abcam
Sourced in United States
The NQO1 activity assays kit is a laboratory tool designed to measure the activity of the enzyme NAD(P)H quinone oxidoreductase 1 (NQO1). NQO1 is an important enzyme involved in cellular defense against oxidative stress. The kit provides a quantitative method to assess NQO1 activity in biological samples.
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NQO1 Activity Assay Protocol
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NQO1 Activity Assay Protocol
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NQO1 activity assays kit (#ab184867) was purchased from Abcam (Cambridge, MA, USA). According to the manufacturer's instructions, cells were lysed with 1X extraction buffer containing 1 mM phenylmethylsulfonyl fluoride for 15 min on ice, and then centrifuged at 17,000 × g at 4°C for 20 min. The supernatant was collected and then the protein concentration of each sample was determined by bicinchoninic acid assays (Santa Cruz Biotechnology). Diluted sample and reaction buffer were added into 96-wells plate containing menadione or isoplumbagin with cofactor NADH and water-soluble tetrazolium salt
The absorbance of reduced water-soluble tetrazolium salt was measured at 450 nm wavelengths by a spectrophotometer.
The absorbance of reduced water-soluble tetrazolium salt was measured at 450 nm wavelengths by a spectrophotometer.
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