Anti mouse cd62l clone mel 14

Manufactured by BD

Anti-mouse CD62L (clone MEL-14) is a monoclonal antibody that binds to the mouse CD62L (L-selectin) cell surface antigen. CD62L is expressed on various immune cells and plays a role in leukocyte trafficking.

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Anti-CD62L (clone MEL-14, (6 citations) CD62L (clone MEL‐14, (7 citations) Anti-CD62L (MEL-14) (9 citations) CD62L (MEL-14; (19 citations) MEL-14 (9 citations) Anti-mouse CD62L (2 citations) Clone 14 (13 citations) Anti-CD62L (31 citations) CD62L (98 citations) Mouse anti (2 citations)

2 protocols using anti mouse cd62l clone mel 14

Quantification of Antigen-Specific CD8+ T Cells

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Allophycocyanin-labelled dextramers specific for OVA H-2Kb (257-SIINFEKL-264) were purchased from Immudex (Copenhagen, Denmark). Samples for flow cytometry were stained with the appropriate antibody cocktails in ice-cold PBS supplemented with 2 mM EDTA and 1% FBS. Anti-mouse CD45 (clone 30F11), CD8α (clone 53-6.7), CD103 (clone 2E7), CD44 (clone IM7), CD45.1 (clone A20) and CD45.2 (clone 104) antibodies were obtained from eBioscience. Anti-mouse CD62L (clone MEL-14) and CD69 (clone H1.2F3) antibodies were obtained from BD Biosciences. Anti-mouse CD279 (PD-1, clone 29F.1A12) was obtained from Biolegend. Events were acquired using an LSRFortessa SORP (Becton Dickinson) flow cytometer or Spectral Cell Analyzer SP6800 (Sony) and data were analysed using FlowJo V10 software (Tree Star).

Enamorado M., Iborra S., Priego E., Cueto F.J., Quintana J.A., Martínez-Cano S., Mejías-Pérez E., Esteban M., Melero I., Hidalgo A, & Sancho D. (2017). Enhanced anti-tumour immunity requires the interplay between resident and circulating memory CD8+ T cells. Nature Communications, 8, 16073.

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Murine Immune Cell Phenotyping

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Anti-IDH2 was from Abcam (# ab131263), anti-IDH1 was from Cell Signaling Technology (#8137S), and anti-HIF1α is from Novus biologicals (#NB100–105). Anti-mouse CD3 (clone 145–2C11, cat#16–0031), anti-mouse CD28 (clone 37.51, cat#16–0281), anti-mouse IFNγ (Clone XMG1.2,Cat#16–7311), anti-mouse IL-4(Clone 11B11, cat#16–7041), anti-mouse FOXP3 (clone FJK-16s, cat#17–5773) are from eBioscience. Anti-mouse CD4 (clone RM4–5, Cat#550954), anti-mouse CD25 (clone 7D4, Cat#558642), anti-mouse CD44 (Clone IM7, Cat#559250), anti-mouse CD62L (clone MEL-14, Cat#560507), anti-mouse IL-17 (clone TC11–18H10, Cat#559502), anti-mouse IFNγ (Clone XMG1.2, Cat#561040, for staining) and anti-CD90.1 (Thy1.1) (clone OX-7, cat#561406) were from BD Bioscience.

Xu T., Stewart K.M., Wang X., Liu K., Xie M., Ryu J.K., Li K., Ma T., Wang H., Ni L., Zhu S., Cao N., Zhu D., Zhang Y., Akassoglou K., Dong C., Driggers E.M, & Ding S. (2017). Metabolic control of TH17/iTreg balance by an epigenetic mechanism. Nature, 548(7666), 228-233.

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