After 0.25% trypsinization, chondrocytes were seeded onto 96-well plates. Upon 60–70% confluence, the cells underwent transfection with miR-532-5p mimic, OE-ABL1, and their negative control (mimic-NC and OE-NC) (GenePharma, Shanghai, China) plasmids using a LipofectamineTM 3000 kit (Invitrogen, Carlsbad, CA, USA) at a concentration of 75 nM. The cells were divided into control, mimic-NC, miR-532-5p mimic, mimic-NC + OE-NC, miR-532-5p mimic + OE-NC, mimic-NC + OE-ABL1, and miR-532-5p mimic + OE-ABL1 groups.
Oe abl1
Manufactured by Thermo Fisher Scientific
Sourced in United States
The OE-ABL1 is a laboratory instrument designed for the detection and analysis of the ABL1 gene, which is associated with certain types of leukemia. The core function of the OE-ABL1 is to provide accurate and reliable data on the expression levels of the ABL1 gene in biological samples, enabling researchers and clinicians to better understand and monitor the progression of these diseases.
Automatically generated - may contain errors
1 protocol using oe abl1
1
Chondrocyte Transfection and miR-532-5p
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