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Macro photo lens mp e 65 mm

Manufactured by Canon
Sourced in Japan

The Canon Macro Photo Lens MP-E 65 mm is a specialized lens designed for close-up photography. It offers a maximum magnification ratio of 5x, allowing users to capture intricate details and small subjects in high resolution. The lens features a fixed focal length of 65 mm and a minimum focus distance of approximately 23 cm, making it well-suited for scientific, industrial, and nature photography applications.

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15 protocols using macro photo lens mp e 65 mm

1

Measuring Sliding Angles of Superhydrophobic Surfaces

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Sliding angles were measured by depositing a 20-μl water droplet on a planar superhydrophobic surface fixed to a tilt platform. Then, the plate was inclined slowly until the droplet started to move. The whole process was imaged by a high-speed camera (Phantom v1610 with Canon macro photo lens MP-E 65 mm) at a frame rate of 1000 frames/s (fps). Sliding angles have been evaluated from the acquired image sequence using MATLAB. Measured sliding angles for all studied liquid systems are shown in fig. S2F.
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2

New Beetle Species Identification Protocol

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Specimens were mainly collected by hand from decayed wood and fungi in broad-leaved forests and killed with ethyl acetate. For examination of the male genitalia, the last two abdominal segments were detached from the body after softening the specimens in hot water. The aedeagi were mounted in Euparal (Chroma Gesellschaft Schmidt, Koengen, Germany) on plastic slides. Photos of the aedeagi were taken with a Canon G9 camera attached to an Olympus SZX 16 stereoscope; habitus photos were taken with a Canon macro photo lens MP-E 65 mm attached to a Canon EOS 7D camera and stacked with Zerene Stacker (http://www.zerenesystems.com/cms/stacker).
The type specimens treated in this study are deposited in the following public and private collections:
NMPC

National Museum, Praha, Prague, Czech Republic

SHNU

Department of Biology, Shanghai Normal University, P. R. China

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3

Genitalia Examination Protocol for Insects

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For examination of the male genitalia, the apical three abdominal segments were detached from the body after softening in hot water. The aedeagi, together with other dissected parts, were mounted in Euparal (Chroma Gesellschaft Schmidt, Koengen, Germany) on plastic slides. Photos of sexual characters were taken with a Canon G9 camera attached to an Olympus CX31 microscope; habitus photos were taken with a Canon macro photo lens MP-E 65 mm attached to a Canon EOS7D camera.
The type specimens treated in this study are deposited in the following collections:
SHNU

Department of Biology, Shanghai Normal University, P. R. China

cPut

Private collection Volker Puthz, Schlitz, Germany

The measurements are abbreviated as follows:
BL

body length, measured from the anterior margin of the clypeus to the posterior margin of abdominal tergite X

FL

forebody length, measured from the anterior margin of the clypeus to the apicolateral angle of elytra

HW

width of head including eyes

PW

width of pronotum

EW

width of elytra

PL

length of pronotum

EL

length of elytra, measured from humeral angle

SL

length of elytral suture

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4

Visualizing Internal Droplet Flow

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For studying the internal flow of liquid droplets, density-matched tracer particles were used (see the fluorescent particle preparation described above). Fluorescent particles were used for better visualization of internal flow due to the semitransparent superhydrophobic coating. The particle motion inside the closed superhydrophobic capillaries were imaged under exposure of ultraviolet (UV) light (10PCS/lot 110V-240V T5 UV lamp 8W Black Light Blue) using a Canon 70D camera at the frame rate of 24 fps. A Canon macro photo lens MP-E 65 mm was used. See the Supplementary Materials for more details. An illustration of the setup is shown in fig. S4.
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5

Superhydrophobic Droplet Imaging

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The droplet was placed by a pipette inside a 4.20–mm–inner diameter superhydrophobic capillary, taking care that the pipette did not touch the inside wall of the capillary. The droplet motion inside the closed superhydrophobic capillary was imaged with a Canon 70D camera. Images were taken at the rate of one image per second using a Canon macro photo lens MP-E 65 mm. For more details, see the Supplementary Materials. An illustration of the setup is shown in fig. S1A.
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6

Bamboo Litter Arthropod Collection

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The specimens, examined in this paper, were collected by searching logs, sifting rotten bamboo or leaf litter and were euthanised with ethyl acetate. For examination of the genitalia, the last three abdominal segments were detached from the body after softening in hot water. The aedeagus or tergite X, together with other dissected pieces, was mounted in Euparal (Chroma Gesellschaft Schmidt, Koengen, Germany) on plastic slides. Photos of sexual characters were taken with a Canon G9 camera attached to an Olympus SZX 16 stereoscope; habitus photos were taken with a Canon macro photo lens MP–E 65 mm attached to a Canon EOS7D camera and stacked with Zerene Stacker.
The specimens treated in this study are deposited in the Department of Biology, Shanghai Normal University, P. R. China (SHNU) and Naturhistorisches Museum Wien, Austria (NMW).
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7

Leaf Litter Beetle Morphometric Analysis

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The specimens examined in this paper were mainly collected at various locations in Zhejiang, East China by sifting leaf litter in broad leaf forests. Specimens were killed with ethyl acetate and dried. For examination of the male and female genitalia, the apical three abdominal segments were detached from the body after softening in hot water. The aedeagi, together with other dissected parts, were mounted in Euparal (Chroma Gesellschaft Schmidt, Koengen, Germany) on plastic slides. Photos of sexual characters were taken with a Canon G9 camera attached to an Olympus CX31 microscope; habitus photos were taken with a Canon macro photo lens MP-E 65 mm attached to a Canon EOS7D camera and stacked with Zerene Stacker.
The type specimens treated in this study are deposited in the following public and private collections:
SHNU Department of Biology, Shanghai Normal University, P. R. China
cPut
Private collection V. Puthz, Schlitz, Germany
The measurements of proportions are abbreviated as follows:
BL body length, measured from the anterior margin of the clypeus to the posterior margin of abdominal tergite X
FL forebody length, measured from the anterior margin of the clypeus to the apicolateral angle of elytra
HW
width of head including eyes
PW width of pronotum
EW width of elytra
PL length of pronotum
EL
length of elytra, measured from humeral angle
SL
length of elytral suture
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8

Leaf Litter Dwelling Rove Beetle Protocols

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The specimens examined in this paper were mainly collected at various locations in Guizhou, southwest China, by sifting leaf litter in broad-leaved forests. Specimens were euthanized with ethyl acetate and dried. For examination of the male and female genitalia, the apical three abdominal segments were detached from the body after softening in hot water. The aedeagi, together with other dissected parts, were mounted in Euparal (Chroma Gesellschaft Schmidt, Koengen, Germany) on plastic slides. Photographs of genitalia were taken with a Canon G9 camera attached to an Olympus CX31 microscope; habitus photos were taken with a Canon macro photo lens MP-E 65 mm attached to a Canon EOS7D camera and stacked with Zerene Stacker.
The type specimens treated in this study are deposited in the following public and private collections:
SHNU Department of Biology, Shanghai Normal University, P. R. China;
cPut Private collection V. Puthz, Schlitz, Germany.
The measurements of proportions are abbreviated as follows:
BL body length, measured from the anterior margin of the clypeus to the posterior margin of abdominal tergite X;
FL fore-body length, measured from the anterior margin of the clypeus to the apicolateral angle of elytra;
HW width of head including eyes;
PW width of pronotum;
EW width of elytra;
PL length of pronotum;
EL length of elytra, measured from humeral angle;
SL length of elytral suture.
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9

Insect Specimen Preparation and Measurement

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The material treated in this study is deposited in the Insect Collection of Shanghai Normal University, Shanghai, China (SNUC).
Type labels are cited in their original spelling. A slash (/) is used to separate different labels.
The specimens were killed with ethyl acetate and then dried. Materials were stored in 75% ethanol; genitalia and small parts were embedded in Euparal on plastic slides that were attached to the same pin with the specimens.
Morphological studies were carried out using an Olympus SZX 16 stereoscope. A digital camera Canon EOS 7D with MP-E 65 mm Macro Photo Lens was used for the habitus photos. An Olympus CX31 microscope and a Canon G9 digital camera were used for the photos of small structures.
The measurements of various body parts are abbreviated as follows: BL

– length of the body from the apical margin of the labrum to the abdominal apex

; HL

– length of the head from the anterior margin of the frons to the posterior constriction

; HW

– maximum width of the head

; PL

– length of the pronotum along midline

; PW

– maximum width of the pronotum

; EL

– length at the suture from the apex of the scutellum to the posterior margin of the elytra

; EW

– maximum width of the elytra

; AL

– length of the aedeagus from the apex of the ventral process to the base of the aedeagal capsule

.
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10

Detailed Insect Imaging Protocol

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Material used in this study is deposited in the Insect Collection of Shanghai Normal University, Shanghai, China (SNUC).
Habitus photos were taken using a Canon EOS 7D camera mounted with an MP-E 65 mm Macro Photo Lens. Photos of dissected parts were taken using a Canon G9 camera mounted on an Olympus CX21 microscope, and combined by the automontage software Zerene Stacker.
Total length was measured from the anterior margin of the head to the posterior margin of the abdominal tenth tergite; forebody length, from the anterior margin of the head to the posterior margin of the elytra; pronotum length, length of the pronotum along the midline; elytra length, length of the elytra along the suture; head width, width of the head across the eyes; pronotum width, maximum width of the pronotum.
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