Biocoat control insert 24 well plate 8.0 μm

Cells were transfected with siRNAs as described above. Transwell chambers were used for cell migration (BioCoat Control Insert 24-well plate 8.0 μm; Corning Inc., Corning, NY, USA) and invasion (BioCoat Matrigel Invasion Chamber 24-well plate 8.0 μm; Corning Inc.) analyses. Cells were harvested 24 h after transfection and resuspended in serum-free RPMI 1640 medium, after which 5 × 10⁴ cells were added to the upper chamber. RPMI 1640 medium with 10% fetal bovine serum was added to the lower well. After incubation for 24 h at 37 °C, migrating or invading cells on the lower surface of the filter were fixed and stained using a Diff-Quik staining kit (Sysmex, Tokyo, Japan). Cell numbers were determined by counting in five randomly selected microscope fields per membrane.

Cells were transfected with plasmids as described above. Transwell chambers were used for cell migration (BioCoat Control Insert 24-well plate 8.0 μm; Corning Inc., Corning, NY, USA) and invasion analyses (BioCoat Matrigel Invasion Chamber 24-well plate 8.0 μm; Corning Inc.). Cells were harvested 24 h after transfection and resuspended in culture medium containing 1 mg/ml bovine serum albumin, after which 1 × 10⁵ cells were added to the upper chamber. Culture medium with 10% fetal bovine serum was added to the lower well. After incubation for 24 h at 37°C, migrating or invading cells on the lower surface of the filter were fixed and stained using a Diff-Quik staining kit (Sysmex, Tokyo, Japan). Cell numbers were determined microscopically by counting in five randomly selected fields per membrane.

Cell migration and invasion assays were performed as described previously^{8 (link)}. Briefly, OSCC cells were transfected with siRNAs as described above and incubated for 48 h. Transwell chambers were used for cell migration (BioCoat Control Insert 24-well plate 8.0 μm; Corning Inc., Corning, NY, USA) and invasion (BioCoat Matrigel Invasion Chamber 24-well plate 8.0 μm; Corning Inc.) analyses. The numbers of migrated or invaded cells were determined microscopically by counting in eight randomly selected microscopic fields per membrane.