Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) activities, and high mobility group box 1 (HMGB1) concentration were measured using the ALT activity assay kit (MAK052; Sigma–Aldrich, St. Louis, MO, USA), AST activity assay kit (MAK055; Sigma–Aldrich), LDH activity assay kit (MAK066; Sigma–Aldrich), and mouse HMGB1 ELISA kit (NBP2-62767; Novus Biologicals, Littleton, CO, USA) according to the manufacturer's protocols, respectively.
Mouse hmgb1 elisa kit
Manufactured by Novus Biologicals
Sourced in United States
The Mouse HMGB1 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse high mobility group box 1 (HMGB1) levels in biological samples.
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4 protocols using mouse hmgb1 elisa kit
1
Serum Biomarkers for Health Assessment
2
Quantifying Inflammatory Markers in Mice
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At the time of sacrifice, blood was harvested in heparin-coated syringes from the inferior vena cava of wild-type mice under terminal isoflurane anesthesia. Plasma was collected after centrifugation of the blood at 10,000 x g for 10 min at room temperature and stored at -80 °C for later analysis. Plasma IL-6 and HMGB1 concentrations were measured using the IL-6 ELISA kit from Millipore corporation (MO, USA) and mouse HMGB-1 Elisa kit (Novus Biologicals (Biotechne, MN, USA), respectively. Plasma samples were diluted twice with the Sample Diluent Buffer of the kit. The standard curve and samples were performed in duplicates. The absorbance was read at 450 nm with an Emax Plus Microplate Reader (Molecular Devices, CA, USA). The standard curve and the samples were performed in duplicates and the absorbance was read at 450 nm.
3
HMGB1 Plasma Quantification by ELISA
4
Quantifying HMGB1 Secretion in CT26 Cells
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The supernatants of CT26 cells were collected after treating with oxaliplatin (10μM or 100 μM) or cisplatin (100 μM) for 24 h. HMGB1 proteins were measured using the mouse HMGB1 ELISA KiT (NBP2-62767, Novus), according to the manufacturer’s instructions.
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