Tricine

Escherichia coli Trans 5α and BL21(DE3) strains and affinity chromatography ProBond resin (Ni²⁺) were bought from TransGen Biotech (Beijing, China) and Promega (Madison, WI), respectively. Trypsase, BamHI and HindIII were merchandised from New England Biolabs (Beijing, China). Tris base of molecular biology grade, NaCl, CaCl₂, anhydrous Na₂CO₃, NH₄HCO₃, NaHCO₃, and NaOH were purchased from Sigma-Aldrich Co. (St Louis, USA). HCl solution (37%), acetonitrile, formic acid (FA), glacial acetic acid were obtained from Thermo Fisher Scientific (Billerica, USA).
Ten percent sodium dodecyl sulfate (SDS) solution, ammonium persulfate, tricine, 5× sample buffer, glycine, 30% acrylamide/bis (29:1), and N,N,N',N' -tetramethylethylenediamine (TEMED) are electrophoresis purity reagents purchased from Bio-Rad Laboratories Inc. (Hercules, USA). Exact-Pro broad range (11-180kDa) pre-stained protein ladder was from Genstar (Beijing, China). Na₂S₂O₃, Coomassie brilliant blue were purchased from Sigma-Aldrich Co. (St Louis, USA). Iso-propyl-thio-β-galactoside (IPTG), kanamycin and imidazole were purchased from Sigma (St Louis, USA). Bacto-yeast extract and Bacto-tryptone were obtained from OXOID (UK).

Commercial O. vulgare essential oil (CO) was purchased from Botanicessence (Product of Spain) (Bangkok, Thailand). Carvacrol, α-tocopherol, 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6, tripyridyl-s-triazine (TPTZ), hydrochloric acid (HCl), acetic acid (CH₃COOH), linoleic acid, hyaluronidase from bovine testes, sodium chloride (NaCl), collagenase from Clostridium histolyticum, N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA), elastase from porcine pancreas, N-succinyl-Ala-Ala-Ala-p-nitroanilide (AAAVPN), sodium phosphate monobasic dihydrate (NaH₂PO₄.2H₂O), and sodium phosphate dibasic dihydrate (Na₂HPO₄.2H₂O) were purchased from Sigma-Aldrich (St. Louis, MO, USA); α-Ascorbic acid was purchased from Asia Pacific Specialty Chemicals Limited (New South Wales, Australia). Anhydrous sodium sulfate (Na₂SO₄), ferric chloride hexahydrate (FeCl₃.6H₂O), ferrous sulphate heptahydrate (FeSO₄.7H₂O), and ammonium thiocyanate (NH₄SCN) were purchased from Loba Chemie (Boisar, Tarapur, India). Bovine serum albumin was purchased from Merck (Darmstadt, Germany). Tricine was purchased from Bio-Rad Laboratories (Richmond, CA, USA). Sodium acetate trihydrate (CH₃COONa.3H₂O), calcium chloride dihydrate (CaCl₂.2H₂O), sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO), dichloromethane, methanol, and ethanol were analytical grade and purchased from RCI Labscan Co., Ltd. (Bangkok, Thailand).