Each plasma sample was separated by centrifugation for 10 min at 3,012.4 g at 4°C. Total cholesterol (TC), triglyceride (TG), and high-density lipoprotein cholesterol (HDL-C) levels were measured using commercial kits (AM 202-K, AM 157S-K, and AM 203-K, respectively; Asan Pharmaceutical Co., Seoul, Korea). LDL cholesterol (LDL-C) levels were calculated using the Friedewald equation (21 (link)).
Am202 k
Manufactured by Asan Pharmaceutical
The AM202-K is a laboratory equipment designed for conducting specific analysis and testing procedures. It is a versatile device that can be utilized for various applications within a research or testing environment. The core function of the AM202-K is to perform precise measurements and data collection, supporting the needs of scientific investigations and quality control processes.
Automatically generated - may contain errors
Lab products found in correlation
Am157s k, by Asan Pharmaceutical (7 mentions)
Am203 k, by Asan Pharmaceutical (4 mentions)
Triton x 100, by Avantor (1 mentions)
Quantikine elisa kit, by R&D Systems (1 mentions)
Am102 k, by Asan Pharmaceutical (1 mentions)
Am103 k, by Asan Pharmaceutical (1 mentions)
Ezrmi 13k, by Merck Group (1 mentions)
Accu chek aviva, by Roche (1 mentions)
Am201 k, by Asan Pharmaceutical (1 mentions)
Ab65341, by Abcam (1 mentions)
Adi 900 019a, by Enzo Life Sciences (1 mentions)
Lf ek0239, by AbFrontier (1 mentions)
Multiskan skyhigh, by Thermo Fisher Scientific (1 mentions)
Csb e07272m, by Cusabio (1 mentions)
Csb e04650m, by Cusabio (1 mentions)
8 protocols using am202 k
1
Plasma Lipid Profile Measurement
2
Hepatic Lipid and Inflammatory Analysis
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Triglycerides and total cholesterol were extracted from the liver tissue with chloroform-methanol (2:1, vol:vol), as previously described [21 (link)]. Briefly, chloroform-methanol was added to homogenized liver tissue, vortexed, and centrifuged; the lower phase was collected and evaporated at room temperature under a fume hood. The resulting semi-dried pellets were then dissolved in 1% Triton X-100 (VWR, USA). The hepatic and serum triglycerides (TG) and total cholesterol (TC) contents were quantified using commercial kits (Asan Pharmaceutical Co., Seoul, Korea, #AM157S-K, #AM202-K). Hepatic functional parameters such as alanine aminotransferase (ALT) and aspartate transaminase (AST) activities were measured using commercial kits (Asan Pharmaceutical Co., Seoul, Korea, #AM102-K, #AM103-K). Serum concentrations of TNF-α and IL-6 were determined using Quantikine ELISA kits (R&D Systems, Minneapolis, MN, USA, #MTA00B, #M6000B).
3
Metabolic Biomarkers Quantification
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Blood glucose levels were measured by Accu-Chek Aviva glucose monitors (Roche Diagnostics, Indianapolis, IN, USA) and plasma insulin was measured using an ELISA kit (cat. no. EZRMI-13K; Millipore, Bedford, MA, USA). Plasma levels of total cholesterol (TC), triglyceride (TG) and HDL-cholesterol were measured using commercially available kits (cat. no’s. AM202-K, AM157S-K and AM203-K, respectively; Asan Pharmaceutical, Seoul, Korea). For liver TG quantification, liver tissues were homogenized and extracted in chloroform, methanol and DW (2/1/1 ratio).
4
Extraction and Analysis of Softshell Turtle
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Fresh softshell turtle (T. sinensis) bodies were obtained from the Gunwi Zara Aquaculture Farm (Ubo, Gunwi, Korea). A voucher specimen is deposited in the laboratory of Dr. Mi-Ryung Kim (Silla University, Busan, Korea). Whole bodies (2 kg) were extracted with boiling water (20 L) for 25 h. After removing the residue and oily layer by centrifugation at 3,000 g for 10 min, the aqueous portion was concentrated using a rotary evaporator to obtain the extract (220 g; yield 11%). The extract was adjusted to 88 mg/mL (or 50 mg/mL as protein) with distilled water. Bovine serum albumin was used as the standard when determining the protein content (8 (link)). Assay kits for determining glucose (AM201-K), urea (AM165-K), lactate dehydrogenase (LDH; LDH-LQ), triglyceride (AM157S-K), and cholesterol (AM202-K) were purchased from Asan Pharmaceutical (Seoul, Korea). The glutathione peroxidase (GPx) kit (K762-100) was purchased from BioVision (Milpitas, CA, USA). The other reagents used in this study were of analytical grade.
5
Lipid Profile Measurement Protocol
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Plasma triglyceride (TG), total cholesterol (TC), and high-density lipoprotein cholesterol (HDL-C) concentrations were measured using commercially available kits (AM157S-K, AM202-K, and AM203-K, respectively; Asan Pharmaceutical Co., Seoul, Korea). Low-density lipoprotein cholesterol (LDL-C) concentration was calculated by the method of Friedewald et al. [21 (link)].
6
Plasma Lipid and Adipokine Profiling
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The levels of plasma TG, TC, and HDL-C were measured using commercially available kits (AM157S-K, AM202-K, and AM203-K; Asan Pharmaceutical Co., Seoul, Korea). NEFA was determined using commercial kits (ab65341; Abcam Inc., Cambridge, MA, USA). Plasma LDL-C level was calculated using a previously reported method [51 (link)] In addition, commercial kits were used to evaluate adipokines such as leptin (#ADI-900-019A; Enzo Life Sciences AG, Lausen, Switzerland) and adiponectin (LF-EK0239; AbFrontier, Seoul, Korea).
7
Biochemical Analysis of Blood Samples
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All the biochemical tests were performed using commercial kits. Whole blood was collected using EDTA vacutainers. Serum was separated by centrifuging the blood at 3000× g 10 min at 4 °C. Triglyceride (TG, AM1575K) and total cholesterol (TC, AM202K) were detected using commercial test kits from Asan Pharmaceutical, Seoul, Republic of Korea. Plasma levels of adiponectin (CSB-E07272m, CUSABIO, Houston, TX, USA) and leptin (CSB-E04650m, CUSABIO, Houston, TX, USA) were determined using commercial sandwich ELISA kits. The absorbance at 450 nm was detected using Multiskan SkyHigh microplate spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The unknown sample concentration (ng/mL) was determined from the standard curve.
8
Blood Lipid Measurement in Mice
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Briefly, 1.0–1.2 ml total blood was collected from each mouse using cardiac puncture following sacrifice. The collected blood was immediately centrifuged (1,000 × g for 30 min at 4°C) to obtain plasma. The plasma levels of triglycerides (TG) and total cholesterol (TC) were measured using commercial kits (AM157S-K for TG and AM 202-K for TC) (Asan Pharmaceutical, Co., Ltd.).
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