Se methylseleno l cysteine mesecys

Chemical reagents. Sodium selenite and SeMet were purchased from Nacalai Tesque (Kyoto, Japan). Se-Methylseleno-l-cysteine (MeSeCys) was purchased from Acros Organics (Waltham, MA, USA). l-Selenocystine (SeCys2) and sodium selenate were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and Wako Pure Chemical Industries, Ltd. (Osaka, Japan), respectively. SeMetO was synthesized based on the method of Kubachka et al. [39 (link)]. 8-Anilino-1-naphthalenesulfonic acid (ANS) and 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) were purchased from Sigma-Aldrich (Shanghai, China). All chemicals were of reagent grade or higher. Ultrapure deionized water (DIW) with 18.3 MΩ cm resistance was used and was prepared using a Milli-Q system (Merck Millipore, Tokyo, Japan).

Sodium selenate and potassium selenocyanate (SeCN⁻) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Sodium selenite and seleno-l-methionine (SeMet) were purchased from Nacalai Tesque (Kyoto, Japan). Se-Methylseleno-l-cysteine (MeSeCys) and l-selenocystine (SeCys₂) were purchased from Acros Organics (Waltham, MA, USA) and Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), respectively. Trimethylselenonium iodide (TMSe⁺) was purchased from Tri Chemical (Uenohara, Japan). l-Selenohomolanthionine (SeHLan) and Se-methylseleno-N-acetylgalactosamine (SeSug1) were synthesized in our laboratory in accordance with our previous work [6 (link),8 (link)]. The chemical structures of the bioselenocompounds used in this study are shown in Figure 1.