Rneasy lipid tissue mini

Manufactured by Qiagen

Sourced in Germany

The RNeasy Lipid Tissue Mini is a lab equipment product designed for the purification of total RNA from lipid-rich tissues. It utilizes a silica-membrane technology to efficiently capture and purify RNA from various sample types. The product's core function is to enable the extraction and isolation of high-quality RNA for downstream applications, such as gene expression analysis, without bias or interpretation.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

RNeasy Mini Kit/RNeasy Lipid Tissue Mini Kit Qiazol RNeasy Lipid Tissue mini kit RNeasy Lipid Tissue Mini Protocol Kit RNeasy Lipid Tissue Mini Kit RNeasy Lipid Tissue RNeasy Mini

5 protocols using rneasy lipid tissue mini

RNA Extraction and qPCR from Visual Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols

Layer IV of visual cortex was manually dissected and RNA was extracted using the RNeasy Lipid Tissue Mini (Qiagen) according to manufacturer’s instructions. cDNA was synthesized from 500 ng of total RNA with the QuantiTect Reverse Transcription kit (Qiagen). For real-time PCR, triplicates were analyzed with a LightCycler 480 II (Roche). Gene-to-GAPDH ratios were determined using the 2^-∆∆Ct method.

Bernard C., Vincent C., Testa D., Bertini E., Ribot J., Di Nardo A.A., Volovitch M, & Prochiantz A. (2016). A Mouse Model for Conditional Secretion of Specific Single-Chain Antibodies Provides Genetic Evidence for Regulation of Cortical Plasticity by a Non-cell Autonomous Homeoprotein Transcription Factor. PLoS Genetics, 12(5), e1006035.

+ Open protocol

+ Expand

Optimized Bone and Marrow RNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole bone as well as the bone fraction were immersed in a lysis solution (1000 μl QIAzol Lysis buffer or 600 μl RLT buffer, both Qiagen), immediately followed by crushing and lysis on the TissueLyser LT (Qiagen). More specifically, the bone tissue was disrupted and homogenized by a 7 mm diameter stainless bead during two consecutive cycles at 50 Hz, each lasting 5′, with an in between 3′ cooling phase on crushed ice. The procedure was highly efficient as <10 % of the overall recovered RNA amount was isolated after the second crushing session of the remaining bone debris (data not shown).
The bone marrow fraction, collected in 600 μl RLT buffer (Qiagen) was disrupted and homogenized by a 5 mm bead at 50 Hz for 3′. The lysate was pipetted into a new sample tube and dissociation of nucleoprotein complexes was promoted by standing the homogenate 5′ on the benchtop.
mRNA isolation was performed using the semi-automatic system QiaCube (Qiagen).
Three commercial extraction kits of Qiagen were tested: RNeasy Lipid Tissue Mini (Lipid) kit, RNeasy Fibrous Tissue Mini (Fibrous) kit, and RNeasy Mini (RNeasy) kit, all followed by a DNase digest step (to make sure all genomic DNA was removed). All samples were eluted in 40 μl RNase-free water and stored at −80 °C until further analysis.

de Loor H., Smout D., Jørgensen H., Meng C., Van Craenenbroeck A.H, & Evenepoel P. (2022). Isolating mineralized bone and bone marrow mRNA from transiliac bone biopsies stored in a stabilizing solution: A comparative study. Bone Reports, 17, 101624.

+ Open protocol

+ Expand

RNA Isolation and Quantification Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated from the liver and AT using RNeasy Mini plus and RNeasy Lipid Tissue Mini kits (Qiagen, Wetburg, Leusden, The Netherlands), respectively, according to the manufacturer's instructions. RNA integrity was determined by agarose gel electrophoresis. RNA quantity (OD-260) and quality (260/OD-280) were determined using an ND-1,000 spectrophotometer (NanoDrop Technologies, Rockland, DE). Total RNA (1 μg) was converted into cDNA using a Quantitect Reverse Transcription kit (Roche, Mannheim, Germany) according to the manufacturer's instructions. Real time PCR (RT-PCR) was performed using a 7900HT system (Applied Biosystems, Warrington, UK) as previously explained [45 (link)] by using Power SYBR Green Master Mix (Roche, Mannheim, Germany). Values were corrected using the housekeeping gene Cyclophillin A (Ppia). Primer sequences are available in Supplementary Table 1.

van der Heijden R.A., Sheedfar F., Morrison M.C., Hommelberg P.P., Kor D., Kloosterhuis N.J., Gruben N., Youssef S.A., de Bruin A., Hofker M.H., Kleemann R., Koonen D.P, & Heeringa P. (2015). High-fat diet induced obesity primes inflammation in adipose tissue prior to liver in C57BL/6j mice. Aging (Albany NY), 7(4), 256-267.

+ Open protocol

+ Expand

Quantitative PCR Analysis of Antioxidant Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted from the cerebral cortex and the liver using RNeasy Lipid Tissue Mini and RNeasy Plus Universal Mini Kit (Qiagen, Hilden, Germany), respectively, following the manufacturer’s protocol. RNA samples were reverse transcribed into cDNA using ReverTra Ace qPCR RT Master Mix with gDNA Remover kit (Toyobo, Osaka, Japan) in accordance with the manufacturer’s protocol. qPCR was conducted in duplicate 10 µL reactions containing 2-µL cDNA, 5-µL TaqMan Fast Advanced Master Mix (Applied Biosystem, Waltham, MA, USA), 0.5-µL TaqMan Gene Expression assay (TXN, Mm00726847_s1; TXNIP, Mm00452393_m1; ACTB, Mm01205647_g1) (Applied Biosystem) and 3.5 µL of RNase free water on a LightCycler 480 instrument (Roche Diagnostic) using the following cycling parameters: Uracil N-glycosylase incubation at 50 °C for 2 min, polymerase activation at 95 °C for 2 min, followed by 40 cycles of denaturation at 95°C for 1 s, annealing/extension at 60 °C for 20 s.

Pahrudin Arrozi A., Abu Bakar Z.H., Taguchi H., Yanagisawa D, & Tooyama I. (2021). A Fluorine-19 Magnetic Resonance Probe, Shiga-Y5, Downregulates Thioredoxin-Interacting Protein Expression in the Brain of a Mouse Model of Alzheimer’s Disease. Molecules, 26(17), 5342.

+ Open protocol

+ Expand

Quantifying Gene Expression in Tumor and Brain Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumors and naïve brains from tumor-bearing or control animals were removed after perfusion with ice-cold Ringer’s solution, snap-frozen, and stored at −80°C until RNA extraction. RNA was extracted using an RNeasy Lipid Tissue Mini (QIAGEN #74804) kit according to the manufacturer’s instructions. RNA concentrations were measured with a NanoDrop (Thermo Scientific) and samples were stored at −80°C. cDNA was synthesized from total RNA using the SuperScript III First-Strand Synthesis System (Invitrogen). Real-time, quantitative PCR (qPCR) was performed using the FastStart SYBR Green Master mix (Roche Applied Science) according to the manufacturer’s instructions. Amplification was performed in the Applied Biosystems 7500 Fast Real-Time PCR System (Life Technologies). Optimized PCR primers were utilized (BioRad). β-actin was used as an internal control and the comparative CT method was used to calculate changes in expression. mRNA expression results were transformed to z-scores and utilized to generate heat-maps and to perform principal component analysis (PCA) in MatLab (MathWorks).

Herting C.J., Chen Z., Pitter K.L., Szulzewsky F., Kaffes I., Kaluzova M., Park J.C., Cimino P.J., Brennan C., Wang B, & Hambardzumyan D. (2017). Genetic driver mutations define the expression signature and microenvironmental composition of high-grade gliomas. Glia, 65(12), 1914-1926.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!