EGCG (purity, 98%) was purchased from Sigma-Aldrich (Merck KGaA). LPS was obtained from Sigma-Aldrich (Merck KGaA). The Cell Titer 96 Aqueous cell viability assay kit was procured from Promega Corporation. ELISA kits for vascular endothelial growth factor (VEGF; cat. no. DY493), Rantes (cat. no. DY478), MCP-1 (cat. no. SMJE00B), ICAM-1 (cat. no. MIC100), matrix metalloproteinase 2 (MMP-2; cat. no. SMMP200) and TNF-α (cat. no. SMTA00B), as well as nitric oxide (NO; cat. no. SKGE001) assay kits were purchased from R&D Systems, Inc. Anti-Akt (cat. no. 4685), anti-nuclear factor-κB (NF-κB) p65 (cat. no. 3034), anti-p38 (cat. no. 9212), anti-Erk (cat. no. 4695), anti-phosphorylated (p)-Akt (cat. no. 4060), anti-p-NF-κB p65 (cat. no. 3033) and anti-p-ERK (cat. no. 4376) were all obtained from Cell Signaling Technology, Inc. Mouse anti-β-actin (cat. no. sc-58673) was obtained from Santa Cruz Biotechnology, Inc.
Cell titer 96 aqueous cell viability assay kit
Manufactured by Promega
Sourced in United States
The Cell Titer 96 Aqueous cell viability assay kit is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The kit utilizes a novel tetrazolium compound which is bio-reduced by cells into a colored formazan product that is soluble in tissue culture medium.
Automatically generated - may contain errors
2 protocols using cell titer 96 aqueous cell viability assay kit
1
EGCG Modulates Inflammatory Pathways
2
Cell Viability Assay with MTS Colorimetric Reagent
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Viability was measured using the Cell Titer 96™ AQueous cell viability assay kit (Promega, Madison, WI, USA). This assay is based on the cellular conversion of the colorimetric reagent MTS (3,4-(5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2 H-tetrazolium salt) in the presence of electron-coupling reagent phenazine methosulfate into soluble formazan by dehydrogenase, which was only found in metabolically active condition in living cells. Formazan formation was measured on the basis of increased absorbance at 490 nm.
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