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Tropix phospha light chemiluminescent assay kit

Manufactured by Thermo Fisher Scientific
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The Tropix Phospha-Light Chemiluminescent Assay Kit is a laboratory instrument designed to detect and quantify phosphatase enzyme activity through a chemiluminescent reaction. It provides a sensitive and reliable method for measuring various phosphatase levels in biological samples.

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Lab products found in correlation

Griptite 293 msr cells, by Thermo Fisher Scientific (2 mentions) Varioskan flash luminometer, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Phospha-Light kit (9 citations) Phospha-Light (4 citations) Tropix kit (2 citations)

2 protocols using tropix phospha light chemiluminescent assay kit

1

Quantifying Chimpanzee Adenovirus Neutralizing Antibodies

Cited 1 time
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Chimpanzee adenovirus ChAdOx1-specific neutralizing antibody titers were assessed using a secreted placental alkaline phosphatase (SEAP) quantitation assay as described6 (link). Briefly, GripTite MSR 293 cells (Invitrogen, catalog no. R795–07) were infected with the serial diluted serum in phenol red–free DMEM (Life Technologies, catalog no. 31053028) and the ChAdOx1-SEAP reporter virus in a 1:1 mixture for 1 hour before replacing with phenol red–free 10% FBS DMEM for 24 hours. For each sample, SEAP concentration was assessed in 50 μl aliquots of culture supernatant, with CPSD as an indicator substrate (Tropix Phospha-Light Chemiluminescent Assay Kit, Life Technologies, catalog no. T1017). Luminescence intensity was measured using a Varioskan Flash luminometer (Thermo Fisher Scientific). Serum dilution neutralization titers were measured by linear interpolation of adjacent values (to 50% inhibition) to determine the serum dilution required to reduce SEAP concentration by 50% compared to wells with virus alone.
van Doremalen N., Lambe T., Spencer A., Belij-Rammerstorfer S., Purushotham J.N., Port J.R., Avanzato V.A., Bushmaker T., Flaxman A., Ulaszewska M., Feldmann F., Allen E.R., Sharpe H., Schulz J., Holbrook M., Okumura A., Meade-White K., Pérez-Pérez L., Edwards N.J., Wright D., Bissett C., Gilbride C., Williamson B.N., Rosenke R., Long D., Ishwarbhai A., Kailath R., Rose L., Morris S., Powers C., Lovaglio J., Hanley P.W., Scott D., Saturday G., de Wit E., Gilbert S.C, & Munster V.J. (2020). ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques. Nature, 586(7830), 578-582.
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2

Quantifying Chimpanzee Adenovirus Neutralizing Antibodies

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Chimpanzee adenovirus ChAdOx1-specific neutralizing antibody titers were assessed using a secreted placental alkaline phosphatase (SEAP) quantitation assay as described6 (link). Briefly, GripTite MSR 293 cells (Invitrogen, catalog no. R795–07) were infected with the serial diluted serum in phenol red–free DMEM (Life Technologies, catalog no. 31053028) and the ChAdOx1-SEAP reporter virus in a 1:1 mixture for 1 hour before replacing with phenol red–free 10% FBS DMEM for 24 hours. For each sample, SEAP concentration was assessed in 50 μl aliquots of culture supernatant, with CPSD as an indicator substrate (Tropix Phospha-Light Chemiluminescent Assay Kit, Life Technologies, catalog no. T1017). Luminescence intensity was measured using a Varioskan Flash luminometer (Thermo Fisher Scientific). Serum dilution neutralization titers were measured by linear interpolation of adjacent values (to 50% inhibition) to determine the serum dilution required to reduce SEAP concentration by 50% compared to wells with virus alone.
van Doremalen N., Lambe T., Spencer A., Belij-Rammerstorfer S., Purushotham J.N., Port J.R., Avanzato V.A., Bushmaker T., Flaxman A., Ulaszewska M., Feldmann F., Allen E.R., Sharpe H., Schulz J., Holbrook M., Okumura A., Meade-White K., Pérez-Pérez L., Edwards N.J., Wright D., Bissett C., Gilbride C., Williamson B.N., Rosenke R., Long D., Ishwarbhai A., Kailath R., Rose L., Morris S., Powers C., Lovaglio J., Hanley P.W., Scott D., Saturday G., de Wit E., Gilbert S.C, & Munster V.J. (2020). ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques. Nature, 586(7830), 578-582.
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