Advia 120 2120 haematology system

Manufactured by Siemens

Sourced in Italy

The ADVIA® 120/2120 Haematology System is a lab equipment product from Siemens. It is a fully automated haematology analyser designed to perform complete blood count (CBC) and differential analysis of blood samples.

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Lab products found in correlation

Immulite, by Siemens (4 mentions) Bntmii system analyzer, by Siemens (2 mentions) Creatinine plus assay, by Roche (1 mentions) Glucose analyzer, by Beckman Coulter (1 mentions) Uricase pod method, by Roche (1 mentions)

5 protocols using advia 120 2120 haematology system

Comprehensive Metabolic Profile Assay

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Hemoglobin, hematocrit, and white blood cell count were measured using an automated particle counter (Siemens Healthcare Diagnostics ADVIA Ò 120/2120 Haematology System, Milan, Italy). Glucose, triglycerides, total and high density lipoprotein (HDL) cholesterol levels were assessed by enzymatic methods (Roche, Basel, Switzerland). HbA1c was measured with high performance liquid chromatography using a national glycohemoglobin standardization program (NGSP) certified automated analyzer (Adams HA-8160 HbA1C analyzer, Menarini, Italy). Fibrinogen was determined by an automated nephelometric technology using the BNTMII System analyzer (Siemens Healthcare, Italy). Plasma insulin levels were assessed with a chemiluminescence-based assay (Immulite Ò , Siemens Healthcare GmbH, Erlangen, Germany).

Marini M.A., Fiorentino T.V., Andreozzi F., Mannino G.C., Perticone M., Sciacqua A., Perticone F, & Sesti G. (2017). Elevated 1-h post-challenge plasma glucose levels in subjects with normal glucose tolerance or impaired glucose tolerance are associated with whole blood viscosity. Acta diabetologica, 54(8).

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Hematological and Metabolic Biomarker Measurement

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Hemoglobin, hematocrit, and white blood cell count were determined using an automated particle counter (Siemens Healthcare Diagnostics ADVIA Ò 120/2120 Haematology System, Milan, Italy). Glucose, triglycerides, total and high density lipoprotein (HDL) cholesterol concentrations were determined by enzymatic methods (Roche, Basel, Switzerland). HbA1c was measured with high performance liquid chromatography using a National Glycohemoglobin Standardization Program (NGSP) certified automated analyzer (Adams HA-8160 HbA1C analyzer, Menarini, Italy). Fibrinogen was measured by an automated nephelometric technology using the BNTMII System analyzer (Siemens Healthcare, Italy). Plasma insulin concentration was measured with a chemiluminescencebased assay (Immulite Ò , Siemens Healthcare GmbH, Erlangen, Germany).

Marini M.A., Fiorentino T.V., Andreozzi F., Mannino G.C., Succurro E., Sciacqua A., Perticone F, & Sesti G. (2017). Hemorheological alterations in adults with prediabetes identified by hemoglobin A1c levels. Nutrition, metabolism, and cardiovascular diseases : NMCD, 27(7).

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Comprehensive Hematological and Metabolic Profiling

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Haemoglobin, mean corpuscular haemoglobin (MCH), and mean corpuscular volume (MCV) were determined using an automated particle counter (Siemens Healthcare Diagnostics ADVIA^® 120/2120 Haematology System, Milan, Italy). Glucose, triglycerides, total and high density lipoprotein (HDL) cholesterol concentrations were determined by enzymatic methods (Roche, Basel, Switzerland). HbA1c was measured with high performance liquid chromatography using a National Glycohemoglobin Standardization Program (NGSP) certified automated analyzer (Adams HA-8160 HbA1C analyzer, Menarini, Italy). High sensitivity C reactive protein (hsCRP) levels were assessed by an automated instrument (CardioPhase^® hsCRP, Milan, Italy). Serum creatinine was measured by a clinical chemistry analyzer (Roche/Hitachi Modular Analytics System, P Module) using the Roche Creatinine Plus assay (Hoffman-La Roche, Basel, Switzerland). Erythrocyte sedimentation rate (ESR) was measured automatically by the stopped-flow technique in a capillary microphotometer (Alifax Test 1 System Polverara, Italy). Plasma insulin concentration was measured with a chemiluminescence-based assay (Immulite^®, Siemens Healthcare GmbH, Erlangen, Germany).

Marini M.A., Mannino G.C., Fiorentino T.V., Andreozzi F., Perticone F, & Sesti G. (2017). A polymorphism at IGF1 locus is associated with anemia. Oncotarget, 8(20), 32398-32406.

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Comprehensive Biomarker Assessment Protocol

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HbA1c levels were assessed with high performance liquid chromatography using a National Glycohemoglobin Standardization Program (NGSP) certified automated analyzer (Adams HA-8160 HbA1C analyzer, Menarini, Italy). Serum and urine creatinine concentrations were measured by an automated technique based on a Creatinine Jaffè compensated method for serum and urine (Roche Diagnostics) implemented in an auto-analyzer. Albuminuria was measured in fresh urine samples by immuneturbidimetry (Roche Diagnostics). An automated particle counter (Siemens Healthcare Diagnostics ADVIA^® 120/2120 Haematology System,Milan,Italy) was employed to measure hemoglobin, hematocrit, and WBC count.
Glucose, triglycerides, total and HDL cholesterol levels were assessed by enzymatic methods (Roche, Basel, Switzerland). Plasma insulin levels were measured with a chemiluminescence-based assay (Immulite^®,Siemens Healthcare GmbH,Erlangen, Germany). Levels of hsCRP were assessed by an automated instrument (CardioPhase^® hsCRP,Milan,Italy).

Fiorentino T.V., Marini M.A., Succurro E., Sciacqua A., Andreozzi F., Perticone F, & Sesti G. (2017). Elevated hemoglobin glycation index identify non-diabetic individuals at increased risk of kidney dysfunction. Oncotarget, 8(45), 79576-79586.

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Metabolic Profile Biomarker Analysis

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All laboratory measurements were carried out on peripheral blood samples after at least 12 h of fasting. Glycemia was determined by the glucose oxidase method (glucose analyzer, BeckmanCoulter, Milan). Creatinine levels were measured using the Jaffe method. The estimation of glomerular filtration rate (eGFR) was based on the new CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) equation [42] (link). Serum uric acid (UA) levels were assessed using URICASE/POD method (Boehringer Mannheim, Mannheim, Germany). Serum levels of high-sensitivity C-reactive protein (hs-CRP) were measured by immunoturbidimetric method automated system (Cardio Phase hs-CRP, Milan, Italy). In addition, glycated hemoglobin (HbA1c) was measured by high-performance liquid chromatography certified by the national glycohemoglobin standardization program (NGSP) and using an automatic analyzer (Adams HA-8160 HbA1c analyzer, Menarini, Italy). Analytical determinations were taken using an automatic particle counter (Siemens Healthcare Diagnostics, ADVIA 120/2120 Haematology System, Milan, Italy) to measure haemoglobin, haematocrit, and white blood cell count. Plasma concentrations of insulin were determined by chemiluminescence test (Roche Diagnostics). Insulin resistance was determined by the homeostasis model assessment (HOMA) index [43] (link).

Condoleo V., Bonfrate L., Armentaro G., Pelaia C., Cassano V., De Marco M., Severini G., Pastura C.A., Miceli S., Maio R., Perticone M., Arturi F., Sesti G, & Sciacqua A. (2023). Effects of continuous positive airway pressure on comprehensive geriatric assessment and cognitive function in elderly patients with obstructive sleep apnea syndrome. Internal and emergency medicine, 18(3).

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