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Mouse cytokine arrays

Manufactured by RayBiotech
Sourced in Gabon

Mouse cytokine arrays are multi-analyte profiling tools used to detect and quantify the relative levels of various cytokines, chemokines, and other proteins in mouse biological samples, such as cell culture supernatants, tissue homogenates, or serum. These arrays provide a comprehensive assessment of the mouse immune system and inflammatory status.

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2 protocols using mouse cytokine arrays

1

Profiling Cytokine Secretomes in Stromal Cells

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Human cytokine arrays (ab133997) were obtained from Abcam (Cambridge, MA). The medium of hTERT-controlstroma and hTERT-cyclin D1stroma was prepared by culturing cells in DMEM with 10% FBS for 48 hrs. Mouse cytokine arrays were purchased from Raybiotech (Norcross, GA). Conditioned medium of cyclin D1+/+ and cyclin D1−/− MEFs was prepared by culturing cells in serum free DMEM for 48 hrs. The experiments were conducted exactly as described in the manufacture’s protocol.
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2

Comprehensive Cytokine Profiling in Lungs

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A KC (Cxcl1) DuoSet ELISA (RnD Systems, Minneapolis MN) was used to determine KC content in whole lung cell homogenates (50 µg). Raybiotech IGF-1 ELISA (100 µg) and Mouse Cytokine Arrays (50 µg; QAM-CAA-1000; RayBiotech, Inc., Norcross, VA) were also used to detect cytokine, chemokine, and growth factor content within whole lung tissue homogenates. The results were analyzed using Prism 6.0 (GraphPad, La Jolla CA), with the exception of the Raybiotech Mouse Cytokine Arrays which were analyzed at RayBiotech, Inc.
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