Sc 348

Manufactured by Santa Cruz Biotechnology

Sourced in United States

SC-348 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed to perform a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

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5 protocols using sc 348

Quantification of Nrg1 and Targets in Mouse Brain Regions

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Total RNA from the olfactory bulb, prefrontal cortex, dorsal striatum, hippocampus, and cerebellum of Nrg1 III tg and WT mice was assayed via standardized quantitative real-time polymerase chain reaction procedure with TaqMan probes to Nrg1 III, and to targets identified by microarray (RIKEN cDNA 181005G02, potassium inwardly rectifying channel member 13, and transthyretin). mRNA expression was normalized to the geometric mean of reference genes (see supplementary materials for full details). Total protein was isolated from the prefrontal cortex and Nrg1 was quantified using western blotting with an antibody specific to the C-terminus of the Nrg1 protein (1:300, sc-348, Santa Cruz Biotechnology, refer to supplementary materials for details).

Olaya J.C., Heusner C.L., Matsumoto M., Sinclair D., Kondo M.A., Karl T, & Shannon Weickert C. (2017). Overexpression of Neuregulin 1 Type III Confers Hippocampal mRNA Alterations and Schizophrenia-Like Behaviors in Mice. Schizophrenia Bulletin, 44(4), 865-875.

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Antibody Characterization for Neuroscience Research

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In addition to the NRG2 antibodies described above, the commercial and custom antibodies against the following proteins were used in this study: ErbB4, mouse monoclonal Ab-77 (Thermo Fisher; 1 μg/ml), rabbit monoclonal mAB10 (1 μg/ml) ^{7 (link)} and rabbit polyclonal custom antibody 5721 (unpublished; 0.4 μg/ml); Kv2.1, mouse monoclonal (clone K89/34; NeuroMab; 1 μg/ml); rabbit polyclonal antibody against NRG1 (SC-348; Santa Cruz Biotechnology; 0.2 μg/ml); anti-V5 epitope tag (AbD Serotech; 1 μg/ml); Bassoon, rabbit polyclonal (Synaptic Systems; 1:1,000); Gephyrin, mouse monoclonal (clone mAb7a; Synaptic Systems; 1:500); PSD95, mouse monoclonal (clone 7E3-1B8; Pierce; 1:500); Calbindin, mouse monoclonal (clone CB-955; Sigma; 1:1,000); GFP, rabbit polyclonal (Molecular Probes; 1:2,000); Erk2, rabbit polyclonal (C-14; Santa Cruz; 0.2 μg/ml); phospho-Erk2, mouse monoclonal (clone E-4; Santa Cruz; 0.2 ug/ml); GAPDH, mouse monoclonal (clone 6C5; Santa Cruz; 0.2 μg/ml); clathrin heavy chain (CHC), mouse monoclonal (SC-12734; Santa Cruz; 0.1 μg/ml); GluN2A, rabbit monoclonal (clone A12W; Millipore; 1:2,000); GluN2B (clone 13; BD Biosciences; 1 μg/ml); GluA1 (AB1504; Millipore; 0.2 μg/ml).

Vullhorst D., Mitchell R.M., Keating C., Roychowdhury S., Karavanova I., Tao-Cheng J.H, & Buonanno A. (2015). A negative feedback loop controls NMDA receptor function in cortical interneurons via neuregulin 2/ErbB4 signalling. Nature Communications, 6, 7222.

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Antibody Characterization for Neuroscience Research

Cited 1 time

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In addition to the NRG2 antibodies described above, commercial and custom antibodies against the following proteins were used in this study: ErbB4, rabbit monoclonal mAB10 (1 μg ml⁻¹; ref. 7 (link)) and rabbit polyclonal custom antibody 5721 (unpublished; 0.4 μg ml⁻¹); Kv2.1, mouse monoclonal (clone K89/34; NeuroMab; 1 μg ml⁻¹); rabbit polyclonal antibody against NRG1 (SC-348; Santa Cruz Biotechnology; 0.2 μg ml⁻¹); anti-V5 epitope tag (AbD Serotech; 1 μg ml⁻¹); Bassoon, rabbit polyclonal (Synaptic Systems; 1:1,000); Gephyrin, mouse monoclonal (clone mAb7a; Synaptic Systems; 1:500); PSD95, mouse monoclonal (clone 7E3-1B8; Pierce; 1:500); Calbindin, mouse monoclonal (clone CB-955; Sigma; 1:1,000); GFP, rabbit polyclonal (Molecular Probes; 1:2,000); Erk2, rabbit polyclonal (C-14; Santa Cruz; 0.2 μg ml⁻¹); phospho-Erk2, mouse monoclonal (clone E-4; Santa Cruz; 0.2 μg ml⁻¹); GAPDH, mouse monoclonal (clone 6C5; Santa Cruz; 0.2 μg ml⁻¹); clathrin heavy chain, mouse monoclonal (SC-12,734; Santa Cruz; 0.1 μg ml⁻¹); GluN2A, rabbit monoclonal (clone A12W; Millipore; 1:2,000); GluN2B (clone 13; BD Biosciences; 1 μg ml⁻¹); and GluA1 (AB1504; Millipore; 0.2 μg ml⁻¹).

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Immunohistochemical Profiling of Neuronal Markers

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Following primary antibodies were used in the study: anti-NRG1 (rabbit; 1:500, sc-348, Santa Cruz, USA), anti-VAChT (guinea pig; 1:250, AB1588, Merck Millipore, USA), anti-ChAT (goat; 1:100, AB144P, Merck Millipore), anti-NeuN (mouse; 1:1000, MAB377, Merck Millipore), anti-Kv2.1 (mouse; 1:250, clone K89/34, NeuroMab, USA), anti m2 muscarinic receptor (rat; 1:500, MAB367, Merck Millipore), anti-GFAP (mouse; 1:500, G3893, Sigma-Aldrich, USA), anti-Iba1 (rabbit; 1:500, 019-19741, Wako, Japan), anti-ErbB3 (rabbit; 1:50, sc-285, Santa Cruz), and anti-ErbB4 (mouse; 1:300, MS-270-P0, Thermo Fisher Scientific, USA).

Lasiene J., Komine O., Fujimori-Tonou N., Powers B., Endo F., Watanabe S., Shijie J., Ravits J., Horner P., Misawa H, & Yamanaka K. (2016). Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons. Acta Neuropathologica Communications, 4, 15.

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Protein expression analysis in homogenates

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TBS/NP40-soluble homogenates were analysed by SDS-PAGE (~15 to 50 μg protein per lane) and western blotting using antibodies to: apoD (sc-34760 or sc-166612, Santa Cruz, 1:5000), apoE (Cat# 178479, Merck Millipore, 1:5,000), APP (WO2 monoclonal provided by Dr Qiao-Xin Li and Prof Colin Masters, University of Melbourne, 1:200), APP C-terminal fragments (A8717, Sigma, 1:10,000), BACE1 (Ab108392, clone EPR3956, Abcam, 1:5,000) NRG-1 C-terminal fragments (SC-348, Santa Cruz, 1:500), HNE (recognizes proteins modified by the aldehydic lipid peroxidation end product, 4-hydroxynonenal) (MAB3248, R&D Systems, 1:2,000) and β-actin (A5060, Sigma, 1:10,000). Signals were detected using species-specific HRP-conjugated secondary antibodies (Dako) and enhanced chemiluminescence and quantified using ImageJ software. Integrated optical density data were normalized to -actin levels and expressed as relative values.

Li H., Ruberu K., Muñoz S.S., Jenner A.M., Spiro A., Zhao H., Rassart E., Sanchez D., Ganfornina M.D., Karl T, & Garner B. (2015). Apolipoprotein D modulates amyloid pathology in APP/PS1 Alzheimer's disease mice. Neurobiology of aging, 36(5).

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