Log In Sign up
The largest database of trusted experimental protocols

Fc500 analyzer

Manufactured by Beckman Coulter
Visit Supplier
Sourced in United States

The FC500 Analyzer is a flow cytometry instrument designed for cell analysis and sorting. It is capable of detecting and measuring various physical and biochemical properties of cells or particles suspended in a fluid stream.

Automatically generated - may contain errors

Lab products found in correlation

Propidium iodide, by Thermo Fisher Scientific (1 mentions) Cxp analysis software, by BD (1 mentions) Rnase a, by Thermo Fisher Scientific (1 mentions) Facsdiva software version, by BD (1 mentions) Kaluza flow cytometry analysis software, by Beckman Coulter (1 mentions) Facsaria 3, by BD (1 mentions) Pi rnase staining buffer, by BD (1 mentions) Elisa test kit, by Diaclone (1 mentions) Clone g44 26, by BD (1 mentions) Fitc annexin 5 apoptosis detection kit 2, by BD (1 mentions) Annexin 5 fitc early apoptosis detection kit, by Cell Signaling Technology (1 mentions) Anti cd4 pe cy7, by BD (1 mentions) Stain buffer, by BD (1 mentions) Cd49f fitc, by BD (1 mentions) Cd24 pe, by BD (1 mentions) Cd44 fitc, by BD (1 mentions) Cd140a, by R&D Systems (1 mentions) Cd106, by BD (1 mentions) Cd140a, by BD (1 mentions) Cd146, by Beckman Coulter (1 mentions)

15 protocols using fc500 analyzer

1

Cell Cycle Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded at 350 000 cells per well onto 6-well plates, treated the next day with the compounds as indicated. At each time point, media was then collected from each individual well and cells were trypsinized for 3 min in the tissue culture incubator. Upon centrifugation at 1000 r.p.m. for 5 min, cell pellet was fixed in ice cold 70% ethanol and stored at 4 °C for at least 24 h. On the day of analysis, the fixed cells were treated with RNase A (Invitrogen, Grand Island, NY, USA), stained with Propidium Iodide (PI) (Invitrogen), and analyzed on a Beckman Coulter FC500 Analyzer (Beckman Coulter) using CXP analysis software (BD Biosciences, San Jose, CA, USA). All experiments were performed at least twice independently.
Wong C., Laddha S.V., Tang L., Vosburgh E., Levine A.J., Normant E., Sandy P., Harris C.R., Chan C.S, & Xu E.Y. (2014). The bromodomain and extra-terminal inhibitor CPI203 enhances the antiproliferative effects of rapamycin on human neuroendocrine tumors. Cell Death & Disease, 5(10), e1450-.
+ Open protocol
+ Expand
2

Evaluating Transfection Efficiency via Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
The transfection efficiency was evaluated 48 hr post-transfection via flow cytometric analysis using a Beckman Coulter FC500 analyzer (Beckman Coulter). GFP (expressed from Cas9/sgRNA and minicircle vector, respectively) and mRuby (expressed from HDR DP) expressing cells were analyzed using the Kaluza Flow Cytometry Analysis Software (Beckman Coulter). Single-cell sorting was performed on a BD FACSAria III (BD Biosciences) upon excitation with a 488 nm wavelength solid-state laser detecting GFP-positive cells. Data analysis was performed using BD FACSDiva software version 8.0.4 (BD Biosciences).
Hainzl S., Peking P., Kocher T., Murauer E.M., Larcher F., Del Rio M., Duarte B., Steiner M., Klausegger A., Bauer J.W., Reichelt J, & Koller U. (2017). COL7A1 Editing via CRISPR/Cas9 in Recessive Dystrophic Epidermolysis Bullosa. Molecular Therapy, 25(11), 2573-2584.
+ Open protocol
+ Expand
3

Quantifying LGR5 Expression in Transfected Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The expression of LGR5 in transfected A549/H1299 cells was measured using the rat anti-human LGR5 antibody (Miltenyi Biotec, Germany) according to the manufacturer’s instructions. The cells were suspended at a density of 1×106 cells/mL in Hank’s balanced salt solution (HBSS) with 1% FCS, incubated with the antibody (10 µL) for 30 min, and then washed once by HBSS with 1% FCS. Flow cytometry was performed on an FC 500 analyzer (Beckman Coulter). Data were analyzed by FlowJo (San Carlos, CA, USA).
Gao F., Xu J.C., You X.R., Gao X., Wei J.L., Li S.X., Zhu C.L, & Yang C. (2019). The biological functions of LGR5 in promoting non-small cell lung cancer progression. Translational Cancer Research, 8(1), 203-211.
+ Open protocol
+ Expand
4

Cell Cycle Analysis of HCC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Control, RU-A1- and C-209-treated HCC cells (1 × 106) were fixed by adding cold 75% ethanol drop by drop with constant agitation. Samples were stored in ethanol overnight at −20 °C. The following day, the alcohol was washed off with PBS and 500 μl of PI/RNase staining buffer (BD Biosciences, #550825) was added to each sample before analysis through a Beckman Coulter FC500 Analyzer.
Bartucci M., Hussein M.S., Huselid E., Flaherty K., Patrizii M., Laddha S.V., Kui C., Bigos R.A., Gilleran J.A., El Ansary M.M., Awad M.A., Kimball S.D., Augeri D.J, & Sabaawy H.E. (2017). Synthesis and Characterization of Novel BMI1 Inhibitors Targeting Cellular Self-Renewal in Hepatocellular Carcinoma. Targeted oncology, 12(4), 449-462.
+ Open protocol
+ Expand
5

Cytotoxicity and Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa or 3T3 cells were plated in triplicated in 96-well microtiter plates at 4 × 104/well one day before the assay. Human peripheral blood mononuclear cells (PBMCs), isolated from the buffy coat fraction of healthy volunteers' peripheral blood by density-gradient centrifugation, or Jurkat E6–1 cells were added in 5:1 effector:target (E:T) ratio on HeLaor 3T3 cells in the presence of purified EgA1 LiTE or EgA1 ATTACK antibodies. After 24 hours aliquots of the culture supernatants were harvested and the levels of IL-2 were measured by using a commercially available ELISA test kit (Diaclone, Besançon, France). Determination of CD69 expression was performed after 24 hours incubation using PE-conjugated anti-CD69 mAb (clone FN50, BD Biosciences, San José, CA, USA) and FITC-conjugated anti-CD3 mAb (clone UCHT1, Abcam) by flow cytometry as described.4 (link) The samples were analyzed with a Beckman-Coulter FC-500 Analyzer.
Harwood S.L., Alvarez-Cienfuegos A., Nuñez-Prado N., Compte M., Hernández-Pérez S., Merino N., Bonet J., Navarro R., Van Bergen en Henegouwen P.M., Lykkemark S., Mikkelsen K., Mølgaard K., Jabs F., Sanz L., Blanco F.J., Roda-Navarro P, & Alvarez-Vallina L. (2017). ATTACK, a novel bispecific T cell-recruiting antibody with trivalent EGFR binding and monovalent CD3 binding for cancer immunotherapy. Oncoimmunology, 7(1), e1377874.
+ Open protocol
+ Expand
6

Cell cycle analysis of SUM159 and MDA-MB-231

Check if the same lab product or an alternative is used in the 5 most similar protocols
SUM159 and MDA-MB-231 cells were incubated with vehicle control or CDDO-Im (10, 100 and 200 nM) for 24 h. The cells were then harvested by trypsinization and fixed in ice-cold 70% ethanol for 30 min. The ethanol-fixed cells were incubated with Ribonuclease A solution (1 µg/ml) and PI (1 mg/ml) for 30 min in the dark at room temperature and analyzed by FC500 Analyzer (Beckman Coulter, Brea, CA).
So J.Y., Lin J.J., Wahler J., Liby K.T., Sporn M.B, & Suh N. (2014). A Synthetic Triterpenoid CDDO-Im Inhibits Tumorsphere Formation by Regulating Stem Cell Signaling Pathways in Triple-Negative Breast Cancer. PLoS ONE, 9(9), e107616.
+ Open protocol
+ Expand
7

Flow Cytometry Analysis of MCF10DCIS Cell Subpopulations

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The detailed procedure was reported previously [24 (link)]. MCF10DCIS cells isolated from mammospheres were stained with antibodies against CD44-APC, Clone G44–26 from BD Biosciences (Cat# 559942, RRID: AB_398683) and CD10-PE from Thermo Fisher Scientific (Cat# 12–0106-41, RRID: AB_10714985). The stained MCF10DCIS cells were analyzed by flow cytometry using an FC500 Analyzer (Beckman Coulter) to determine the percentage of 4 different CD44−/CD10+, CD44+/CD10+, CD44−/CD10− and CD44+/CD10−subpopulations.
Shan N.L., Minden A., Furmanski P., Bak M.J., Cai L., Wernyj R., Sargsyan D., Cheng D., Wu R., Kuo H.C., Li S.N., Fang M., Maehr H., Kong A.N, & Suh N. (2020). Analysis of the Transcriptome: Regulation of Cancer Stemness in Breast Ductal Carcinoma In Situ by Vitamin D Compounds. Cancer prevention research (Philadelphia, Pa.), 13(8), 673-686.
+ Open protocol
+ Expand
8

CDDO-Im Induces Apoptosis in SUM159 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
SUM159 cells were incubated with vehicle control or CDDO-Im (10, 50, 100, 150 or 200 nM) for 24 h. Cells were harvested by trypsinization, and the dead cells in culture medium were also collected by centrifugation. The collected cells were stained with Annexin V and PI using the FITC Annexin V Apoptosis Detection Kit II (BD Pharmigen, San Jose, CA), following the manufacturer’s instruction. The stained cells were analyzed by FC500 Analyzer (Beckman Coulter).
So J.Y., Lin J.J., Wahler J., Liby K.T., Sporn M.B, & Suh N. (2014). A Synthetic Triterpenoid CDDO-Im Inhibits Tumorsphere Formation by Regulating Stem Cell Signaling Pathways in Triple-Negative Breast Cancer. PLoS ONE, 9(9), e107616.
+ Open protocol
+ Expand
9

Apoptosis Detection in HCC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Control, RU-A1-, C-209-, and camptothecin-treated HCC cells (1 × 106) were washed and stained with Annexin VFITC Early Apoptosis Detection kit (Cell Signaling, #6592) according to the manufacturer’s instructions before analysis on a Beckman Coulter FC500 Analyzer.
Bartucci M., Hussein M.S., Huselid E., Flaherty K., Patrizii M., Laddha S.V., Kui C., Bigos R.A., Gilleran J.A., El Ansary M.M., Awad M.A., Kimball S.D., Augeri D.J, & Sabaawy H.E. (2017). Synthesis and Characterization of Novel BMI1 Inhibitors Targeting Cellular Self-Renewal in Hepatocellular Carcinoma. Targeted oncology, 12(4), 449-462.
+ Open protocol
+ Expand
10

Flow Cytometric Analysis of Lymphocyte Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells (1×106 cells/ml) were washed twice with Stain Buffer (BD Pharmingen) and resuspended in 50 µl Stain Buffer (BD Pharmingen) containing surface markers PE Cy7-anti-CD4 (BD Pharmingen), PC5.5-anti-CD8 (Beckman Coulter, Brea, CA), or appropriate isotype control was added. After 30 min on ice, cells were washed twice with Stain Buffer. These cells were then stained with Acridine orange, Cyto-ID Green, TUNEL, or Annexin-V staining (as below) and analyzed by flow cytometry (Beckman-Coulter FC-500 Analyzer) using Beckman-Coulter Kaluza software. CD4+ and CD8+ cells were determined after gating on a population of lymphocytes in which 3×104 to 5×104 cells per sample were counted and processed for Acridine orange, Cyto-ID Green, TUNEL, or Annexin-V assays.
Lin C.W., Lo S., Hsu C., Hsieh C.H., Chang Y.F., Hou B.S., Kao Y.H., Lin C.C., Yu M.L., Yuan S.S, & Hsieh Y.C. (2014). T-Cell Autophagy Deficiency Increases Mortality and Suppresses Immune Responses after Sepsis. PLoS ONE, 9(7), e102066.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!