AST-3424 was provided by OBI Pharmaceuticals, Inc. (Asia). AST-3424 was dissolved in absolute ethyl alcohol at a concentration of 100 mM and stored at −20°C. Oxaliplatin (OXA) was purchased from Jiangsu Hengrui Medicine Co., Ltd. (Nanjing, China) and then dissolved in GS before use. 5-Fluorouracil (5-Fu) was purchased from Shanghai Xudong Medicine Co., Ltd. (Shanghai, China) and then dissolved in NS before use. The antibodies of Bax, β-catenin, Caspase3, Bcl-2, PARP, AKR1C3, P21, Smad2/3, MMP-2, and MMP-9 were purchased from GeneTex (Irvine, CA, USA). pSmad2/3 antibody was purchased from Cell Signaling Technology (Beverly, MA, USA). Tubulin antibody and TGF-β growth factor were purchased from Jiangsu Keygen Biotech Corp., Ltd. (Nanjing, China).
Psmad2 3 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The PSmad2/3 antibody is a laboratory reagent used to detect phosphorylated Smad2 and Smad3 proteins in cellular samples. Smad2 and Smad3 are key intracellular mediators of the transforming growth factor-beta (TGF-β) signaling pathway. This antibody can be used to monitor the activation state of the TGF-β pathway in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
β catenin, by GeneTex (1 mentions)
Oxaliplatin oxa, by Jiangsu Hengrui Medicine (1 mentions)
Bcl 2, by GeneTex (1 mentions)
Caspase 3, by GeneTex (1 mentions)
Bcl2 associated x (bax), by GeneTex (1 mentions)
Jc 1 mitochondrial membrane potential assay kit, by Beyotime (1 mentions)
Mitosox red, by Thermo Fisher Scientific (1 mentions)
Collagen 3, by Proteintech (1 mentions)
Collagen 1, by Proteintech (1 mentions)
Smad3, by Proteintech (1 mentions)
Smad2, by Proteintech (1 mentions)
Gapdh, by Proteintech (1 mentions)
Tgf β, by Proteintech (1 mentions)
2 protocols using psmad2 3 antibody
1
Investigating AST-3424 and Chemotherapies
2
Investigating Oxidative Stress Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Nrf2, HO-1, TGF-β, Smad-2, Smad-3, Collagen I, Collagen III, and GAPDH antibodies were purchased from Proteintech (Wuhan, China). The p-Smad2/3 antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). Angiotensin II and ROS fluorescent probe DHE were obtained from Aladdin (Shanghai, China), and the neferine and zinc protoporphyrin (ZnPP, HO-1 inhibitor) were obtained from MedChemExpress (Monmouth Junction, NJ, USA). The mito-SOX Red was obtained from Invitrogen (Thermo Fisher Scientific, USA, cat#: M36008). The superoxide dismutase and malondialdehyde (MDA) assay kits were obtained from Solarbio (Beijing, China). The glutathione (GSH) assay kit and JC1 mitochondrial membrane potential assay kit were obtained from Beyotime Biotechnology (Shanghai, China). The atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) ELISA kits were obtained from Elabscience Biotechnology (Wuhan, China).
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