Isovanillin

Manufactured by Merck Group

Sourced in United States

Isovanillin is a chemical compound that serves as a key ingredient in the production of various laboratory equipment and supplies. It is a white crystalline solid with a characteristic vanilla-like aroma. Isovanillin is commonly used as a precursor and building block in the synthesis of other chemical compounds and materials utilized in various scientific and industrial applications.

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Lab products found in correlation

5 protocols using isovanillin

Tissue Bath Pharmacology Assay

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Drugs used were as follows: Acetylcholine, 5-hydroxytriptamine (5-HT), and propantheline (Sigma, Germany), lidocaine (Pasteur, Tehran) isovanillin and isoacetovanillon (Sigma, China).
Acetylcholine (250 μM), 5-HT (100 μM), lidocaine (7.4 mM) and propantheline (1 mM) stock solutions were made up in distilled water. isovanillin and isoacetovanillon were made up as 20 mg/ml stock solution in dimethyl sulphoxide (DMSO). Further serial dilutions were prepared in distilled water. Tyrode's solution with the following composition: NaCl; 136.9, KCl; 2.68, CaCl2;1.8, MgCl2; 1.05, NaHCO3; 11.9, NaH₂PO4; 0.42 and glucose 5.55 mM were made up in distilled water. All the chemicals were from Merck (Germany) unless stated otherwise.

Sadraei H., Ghanadian M., Asghari G, & Madadi E. (2014). Antispasmodic activity of isovanillin and isoacetovanillon in comparison with Pycnocycla spinosa Decne.exBoiss extract on rat ileum. Research in Pharmaceutical Sciences, 9(3), 187-192.

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Comprehensive Analysis of Natural Phenolic Compounds

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Gallic acid (GA), trans-caffeic acid (CA) and its methylated products (3-hydroxy-4-methoxycinnamic acid (trans), trans-ferulic acid), 3,4-dihydrobenzaldehyde (DHA) and its methylated products (vanillin, iso-vanillin), protocatechuic acid (PCA), dopamine hydrochloride and its methylated products (3-hydroxy-4-methoxy-phenethylamine hydrochloride, 3-methoxy-tyramine hydrochloride), quercetin, and luteolin were obtained from Sigma-Aldrich (USA). Two major methylated products of GA (3,4-dihydroxy-5-methoxybenzoic acid (5OMeBA), 3,5-dihydroxy-4-methoxybenzoic acid), methylated products of 5OMeBA (5-hydroxyveratric acid, syringic acid), methylated products of PCA (3-hydroxy-4-methoxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid), 5-hydroxyuridine (ho⁵U) and the methylated product 5-methoxyuridine (mo⁵U) were purchased from Carbosynth (UK).

Lee S., Kang J, & Kim J. (2019). Structural and biochemical characterization of Rv0187, an O-methyltransferase from Mycobacterium tuberculosis. Scientific Reports, 9, 8059.

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Quantification of Polyphenol Standards

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Commercial standards of cyanidin-3-O-glucoside chloride, delphindin-3-O-glucoside chloride, malvidin-3-O-glucoside chloride, gallic acid, caffeic acid, ferulic acid, ethyl gallate, taxifolin, chlorogenic acid, hippuric acid, 3-hydroxyhippuric acid, 4-hydroxybenzaldehyde, isovanillin, p-anisic acid, 4-hydroxyphenylacetic acid, 3-hydroxyphenylpropionic acid, 3-methoxyphenylacetic acid, isovanillic acid, homovanillic acid, 3-hydroxy-4-methoxyphenylpropionic acid, syringic acid, quercetin, myricetin, chlorogenic acid, quercetin-3-O-glucuronide, protocatechuic acid, p-coumaric acid, catechin, epicatechin, 4-methoxyquercetin, and quercetin-3-O-glucoside as well as sodium carbonate and Folin and Ciocalteu’s reagent (2N) were purchased from Sigma-Aldrich (St. Louis, MO, USA). caffeic acid glucuronide was supplied by Synthose (Concord, Ontario, Canada). LC-MS grade solvents, including methanol, water, ACN, and formic acid as well as trace metal grade concentrated nitric acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). ⁴⁵Ca was purchased from PerkinElmer (Waltham, MA, USA). EcoLite (+) scintillation cocktail was purchased from MP Biomedicals (Santa Ana, CA, USA).

Cladis D.P., Debelo H., Lachcik P.J., Ferruzzi M.G, & Weaver C.M. (2020). INCREASING DOSES OF BLUEBERRY POLYPHENOLS ALTERS COLONIC METABOLISM AND CALCIUM ABSORPTION IN OVARIECTOMIZED RATS. Molecular nutrition & food research, 64(12), e2000031.

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Quantitative Analysis of Blueberry Polyphenols

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Commercial standards of cyanidin-3-O-glucoside chloride, malvidin-3-O-glucoside chloride, peonidin-3-O-glucoside chloride, petunidin-3-O-glucoside chloride, gallic acid, caffeic acid, ferulic acid, ethyl gallate, taxifolin, chlorogenic acid, 4-hydroxybenzoic acid, 3-hydroxybenzaldehyde, hippuric acid, 3-hydroxyhippuric acid, isovanillin, 4-hydroxyphenylacetic acid, 3-hydroxyphenylpropionic acid, 3-methoxyphenylacetic acid, vanillic acid, homovanillic acid, syringic acid, quercetin, myricetin, quercetin-3-O-glucuronide, 4-methoxyquerceting, p-coumaric acid, epicatechin, kaempferol, and quercetin-3-O-rutinoside as well as sodium carbonate and Folin and Ciocalteu’s reagent (2N) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Extraction and LC-MS grade solvents, including methanol, water, acetonitrile, hexanes, and formic acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). VitaBlue Pure American Blueberry Extract was obtained from FutureCeuticals (Momence, IL, USA); lyophilized composite wild blueberry (Vaccinium angustifolium) powder was obtained from the Wild Blueberry Association of North America (Old Towne, ME, USA).

Cladis D.P., Simpson A.M., Cooper K.J., Nakatsu C.H., Ferruzzi M.G, & Weaver C.M. (2021). BLUEBERRY POLYPHENOLS ALTER GUT MICROBIOTA & PHENOLIC METABOLISM IN RATS. Food & function, 12(6), 2442-2456.

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Phytochemical Analysis of Plant Extracts

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All solvents and reference standards were of analytical grade. Acetonitrile, absolute ethyl alcohol, and formic acid were of HPLC grade (VWR International Ltd, Milan, Italy). Pure standard of apigenin, caffeic acid, (+)-catechin, trans-cinnamic acid, p-coumaric acid, daidzein, trans-ferulic acid, formononetin (7-hydroxy-4'-methoxyisoflavone), gallic acid, iso vanillin, iso vitexin (apigenin-6-C-glucoside), orientin (luteolin-8-C-glucoside), salicylic acid, sinapic acid, syringic acid, vanillic acid, vanillin, vitexin (apigenin-8-C-glucoside), as well as Folin-Ciocâlteu reagent and sodium carbonate were purchased from Sigma-Aldrich ® (Milan, Italy). Deionized water was obtained through an Elix purification system (Millipore, Milan, Italy).

Montevecchi G., Setti L., Olmi L., Buti M., Laviano L., Antonelli A, & Sgarbi E. (2019). Determination of Free Soluble Phenolic Compounds in Grains of Ancient Wheat Varieties ( Triticum sp. pl.) by Liquid Chromatography-Tandem Mass Spectrometry. Journal of agricultural and food chemistry, 67(1).

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