Canoco 4.5 software package

Manufactured by Microcomputer Power

Sourced in United States

CANOCO 4.5 is a software package for multivariate data analysis, specifically designed for ecological applications. It provides tools for ordination, gradient analysis, and related techniques to explore the relationships between species and environmental variables.

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Canoco 4.5 Canoco 5.0 CANOCO software CANOCO

2 protocols using canoco 4.5 software package

Microbial Community Analysis via CCA

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A constrained ordination was carried out by a Canonical Correspondence Analysis (CCA) to correlate environmental variables with microbial phyla and samples. A Monte Carlo test with 499 permutations was carried out to ensure the significance of canonical axes. CANOCO 4.5 software package (Microcomputer Power, Ithaca, NY, USA) was used to perform the CCA and the tool CANODRAW for triplot visualization (ter Braak and Smilauer, 2002 ).

Fernandez A.B., Rasuk M.C., Visscher P.T., Contreras M., Novoa F., Poire D.G., Patterson M.M., Ventosa A, & Farias M.E. (2016). Microbial Diversity in Sediment Ecosystems (Evaporites Domes, Microbial Mats, and Crusts) of Hypersaline Laguna Tebenquiche, Salar de Atacama, Chile. Frontiers in Microbiology, 7, 1284.

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Canonical Correspondence Analysis of Microbiome

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A constrained ordination was carried out by a canonical correspondence analysis (CCA) to correlate environmental variables with prokaryotic phyla and samples. A Monte Carlo test with 499 permutations was made to ensure the significance of canonical axes. CANOCO 4.5 software package (Microcomputer Power, Ithaca, NY, USA) was used to perform the CCA and the tool CANODRAW for triplot visualization [31]

Rasuk M.C., Fernández A.B., Kurth D., Contreras M., Novoa F., Poiré D, & Farías M.E. (2016). Bacterial Diversity in Microbial Mats and Sediments from the Atacama Desert. Microbial ecology, 71(1).

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