Ncf1 antibody

Fetal bovine serum (FBS) was purchased from BioWhittaker Inc. (Walkersville, MO, USA). The following antibodies were purchased: p-PKC, caspase-9, cyclin D1, cyclin E, CDK2, CDK4, cleaved caspase-3, p53, and PKC antibodies (from Cell Signaling Technology, Danvers, MA, USA); NOX2 antibody (from BD Biosciences, Franklin Lakes, NJ, USA); NCF1 antibody (from LifeSpan Biosciences, Seattle, WA, USA); p-ERK1/2, ERK, p-JNK, JNK, p-p38, p38, p-NF-κBp65, NF-κBp65, p-AKT, AKT, β-actin, pan-cadherin (Pan-cad), PKCα, PKCδ, PKCζ, Bcl-2, and Bax antibodies (from Santa Cruz Biotechnology, Paso Robles, CA, USA); and horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse immunoglobulin G (from Jackson Immunoresearch, West Grove, PA, USA). 2′, 7′-dichlorofluorescein diacetate (CM-H₂DCFDA) was obtained from Invitrogen (Carlsbad, CA, USA). A23187, MβCD, NAC, PD98059, SP600125, and CTB were purchased from Sigma Chemical Company (St. Louis, MO, USA). All of the pharmacological inhibitors listed did not show any significant cytotoxic effects by themselves as confirmed by FACS analysis in each experiment. All other reagents were of the highest purity, commercially available, and were used as received.

Fetal bovine serum (FBS) was purchased from BioWhittaker (Walkersville, MO, USA). The following antibodies were purchased: p-PKC and PKC antibodies (Cell Signaling Technology, Danvers, MA, USA); NOX2 antibody (BD Biosciences, Franklin Lakes, NJ, USA); NCF1 antibody (LifeSpan Biosciences, Seattle, WA, USA); ITLN and Na⁺/K⁺ ATPase antibodies (abcam, Cambridge, MA, USA); p-ERK1/2, ERK, p-JNK, JNK, p-p38 MAPK, p38 MAPK, p-c-Src, c-Src, p-FAK, FAK, pan-cadherin, PKCα, PKCδ, and PKCζ antibodies (Santa Cruz Biotechnology, Paso Robles, CA, USA); Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse IgG antibodies (Jackson Immunoresearch, West Grove, PA, USA); 2′, 7′-dichlorofluorescein diacetate (CM-H₂DCFDA) was obtained from Invitrogen (Carlsbad, CA, USA). A23187, methyl-β-cyclodextrin (MβCD), N-acetyl-l-cysteine (NAC) and 5-azacytidine were purchased from Sigma Chemical Company (St. Louis, MO, USA). The concentrations of all of pharmacological inhibitors listed did not show any significant cytotoxic effects by themselves as confirmed by FACS analysis in each experiment. All other reagents were of the highest purity commercially available and were used as received.

Fetal bovine serum (FBS) was purchased from BioWhittaker Inc. (Walkersville, MO, USA). The following antibodies were purchased: NOX2, Rac1, and ANXA2 antibodies (BD Biosciences, Franklin Lakes, NJ, USA); NCF1 antibody (LifeSpan Biosciences, Seattle, WA, USA); p-JNK, JNK, PKCα^{Ser 657}, p-NF-κBp65, NF-κBp65, IL-1β p17, caspase-1 p10, and β-actin antibodies (Santa Cruz Biotechnology, Paso Robles, CA, USA); Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse IgG antibodies (Jackson Immunoresearch, West Grove, PA, USA). 2′, 7′-dichlorofluorescein diacetate (CM-H₂DCFDA) was obtained from Invitrogen (Carlsbad, CA, USA). Methyl-β-cyclodextrin (MβCD), lipopolysaccharide (LPS), adenosine 5′-triphosphate disodium salt hydrate (ATP), 3-methyladenine, bafilomycin A1, N-acetyl-l-cysteine (NAC), rotenone, and 5-azacytidine were purchased from Sigma Chemical Company (St. Louis, MO, USA). The concentrations of all of pharmacological inhibitors listed did not show any significant cytotoxic effects by themselves as confirmed by FACS analysis in each experiment. All other reagents were of the highest purity commercially available and were used as received.