Blood samples (EDTA) were stained with the following monoclonal antibodies: CD3-eFluor605, CD4-eFluor450, TCRγδ-PE (eBioscience), CD45RO-FITC, CCR7-PE-Cy7, CD4-PerCP, CD8-PerCP, CD8-APC-H7, DNAX accessory molecule-1 (DNAM-1)-FITC (BD), CD161-PE, CD161-APC (Miltenyi), 2B4-PE, NKG2D-PE-Cy7, TCR-Vα7.2-FITC, TCR-Vα24-Jα18-FITC (Biolegend), TCR-Vβ11-PE (Beckman Coulter), and KLRG1-FITC (generous gift from H. Pirchner). An overview of antibody panels is shown in Table S1 in Supplementary Material. Samples were subsequently treated with BD Lysing Solution (BD Biosciences) according to instructions of the manufacturer. Samples were measured on a LSR-II flow cytometer (BD) and analyzed with Kaluza Analysis Software (Beckman Coulter). In addition, the absolute numbers of circulating lymphocyte subsets were determined according to the MultiTest TruCount method (BD), as described by the manufacturer. Data were acquired on a FACSCanto-II flow cytometer (BD) and analyzed with FACSCanto Clinical Software (BD). The number of events for a particular T cell population needed to be more than 100 to allow for subsequent analysis of cellular markers, cytokines, and cytotoxic molecules.
Tcrγδ pe
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TCRγδ-PE is a laboratory instrument designed for the detection and analysis of gamma/delta T cells. It utilizes flow cytometry technology to identify and quantify the presence of gamma/delta T cell receptors on the surface of cells. The TCRγδ-PE provides researchers with a tool to study the role of gamma/delta T cells in various biological processes and disease states.
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Lab products found in correlation
Ccr7 pe cy7, by BD (1 mentions)
Cd3 efluor 605, by Thermo Fisher Scientific (1 mentions)
Cd4 efluor450, by Thermo Fisher Scientific (1 mentions)
Cd161 pe, by Miltenyi Biotec (1 mentions)
Multitest trucount method, by BD (1 mentions)
Cd45ro fitc, by Thermo Fisher Scientific (1 mentions)
Cd161 apc, by Miltenyi Biotec (1 mentions)
Nkg2d pe cy7, by BioLegend (1 mentions)
Facscanto clinical software, by BD (1 mentions)
Kaluza analysis software, by Beckman Coulter (1 mentions)
Lysing solution, by BD (1 mentions)
Cd8 apc h7, by BD (1 mentions)
Cd8 percp, by BD (1 mentions)
Cd4 percp, by BD (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Cd8 pe, by Thermo Fisher Scientific (1 mentions)
Facscan flow cytometer, by BD (1 mentions)
Cd20 pe, by Thermo Fisher Scientific (1 mentions)
Cd4 fitc, by Thermo Fisher Scientific (1 mentions)
2 protocols using tcrγδ pe
1
Multiparametric Flow Cytometry Analysis
2
Immunophenotyping of Intestinal Intraepithelial Lymphocytes
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Cells from the above preparations were diluted to 0.5–1 × 106 cells/ml and labeled according to the single-, two-, or three-color staining procedure using FITC, allophycocyanin (APC), Perp-cy5.5, and phycoerythrin (PE)-labeled mAbs, including CD45-Perp-cy5.5, CD3-APC (BD Pharmingen, San Diego, CA, USA), CD8-PE, CD4-FITC, T-cell receptor (TCR) αβ-PE, TCR γδ-PE, CD20-PE (e-Bioscience, San Diego, CA, USA). Appropriate isotype-matched mAbs were used as negative controls. Labeled IELs were analyzed on an FACScan flow cytometer (Becton Dickinson, CA, USA).
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