Log In Sign up
The largest database of trusted experimental protocols

Anti mouse and anti rabbit hrp conjugated secondary antibodies

Manufactured by Bio-Rad
Visit Supplier
Sourced in United States

Anti-mouse and anti-rabbit HRP conjugated secondary antibodies are protein conjugates that bind to the primary antibody targeting mouse or rabbit antigens. The horseradish peroxidase (HRP) enzyme conjugated to the secondary antibody can be used to detect and visualize the target antigen in various immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Snail, by Cell Signaling Technology (2 mentions) N cadherin, by Cell Signaling Technology (2 mentions) Du145, by American Type Culture Collection (2 mentions) Anti phospho akt ser473 9271, by Cell Signaling Technology (1 mentions) Westar nova 2011, by Cyanagen (1 mentions) Anti cdk4 c22, by Santa Cruz Biotechnology (1 mentions) Protease inhibitor cocktail and phosphatase inhibitor cocktail, by Roche (1 mentions) Phospho rtk array, by R&D Systems (1 mentions) C myc 5605, by Santa Cruz Biotechnology (1 mentions) Endothelial cell basal medium 2, by Lonza (1 mentions) Pgsk3 ser 9 21, by Cell Signaling Technology (1 mentions) Microvascular endothelial cell growth medium 2 bullet kit, by Lonza (1 mentions) Lncap, by American Type Culture Collection (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cleaved caspase 9, by Cell Signaling Technology (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Tgf β1, by Cell Signaling Technology (1 mentions) Rhtgf β1, by R&D Systems (1 mentions) P p38 mapk, by Cell Signaling Technology (1 mentions)

5 protocols using anti mouse and anti rabbit hrp conjugated secondary antibodies

1

Western Blot Analysis of Phosphorylated Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer, separated by SDS–PAGE and transferred to nitrocellulose membranes following standard protocols. The membranes were incubated overnight with the following primary antibodies: anti-phospho-Src (Tyr416) (2101, 1:1000), anti-phospho-AKT (Ser473) (9271; 1:1000) and anti-phospho-SMAD3 (Ser423/425) (9520, 1:1000) (Cell Signalling Technology Boston, USA); anti–Tubulin (TU-02, 1:1000), and anti–CDK4 (c-22; 1:1000) (Santa Cruz Biotechnology, Inc., CA, USA). Blots were incubated with HRP-conjugated anti-rabbit and anti-mouse secondary antibodies (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Immunoreactivity was detected by Enhanced Chemiluminescence reaction (WESTAR NOVA 2011, Cyanagen, Bologna, Italy) following the manufacturer’s instructions.
De Santis Puzzonia M., Cozzolino A.M., Grassi G., Bisceglia F., Strippoli R., Guarguaglini G., Citarella F., Sacchetti B., Tripodi M., Marchetti A, & Amicone L. (2016). TGFbeta Induces Binucleation/Polyploidization in Hepatocytes through a Src-Dependent Cytokinesis Failure. PLoS ONE, 11(11), e0167158.
+ Open protocol
+ Expand
2

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed with ice-cold PBS and then lysed with RIPA lysis buffer (150 mmol/L Tris, pH 7.4, 100 mmol/L NaF, 120 mmol/L NaCl, 100 µmol/L sodium orthovanadate, and 1× protease inhibitor cocktail and phosphatase inhibitor cocktail; Roche). Lysates (30 µg) were resolved by SDS-PAGE and transferred to nitrocellulose membranes; which were incubated with primary antibodies at 4°C overnight or at room temperature for 2 hours followed by incubation with HRP-conjugated anti-rabbit and anti-mouse secondary antibodies (Bio-Rad) for 1 hour at room temperature. Immunoreactive bands were visualized by chemiluminescence (Santa Cruz Biotechnology). All primary antibodies were from Cell Signaling Technology with the exception of BRD4 (Abcam), Actin (Abcam) and AXL (Life Technologies). For phospho-RTK arrays (R&D Systems), lysates were procured and applied to arrays according to manufacturer’s instructions. Arrays were visualized using chemiluminescence. Antibodies used for immunoblotting were from Cell Signaling Technology (P-MET-Y1234 #3126, P-CDK9 #2549, P21 #2947, HER3 #12708, P-HER3-Y1289 #4791, ROR2 #88639, HER2 #2242, P-SFK-Y419 #6943, C-MYC #5605, P-MAPK #9101, GAPDH #5174), Santa Cruz Biotechnology (V5 probe #sc-271944), R&D Systems (P-AXL-Y779 #AF2228, AXL #AF154), Abcam (BRD4 #128874, c-MET #59884, Actin #6276, Beta-tubulin #6046) and BD Biosciences (EGFR #610016).
Leonard B., Brand T.M., O’Keefe R.A., Lee E.D., Zeng Y., Kemmer J.D., Li H., Grandis J.R, & Bhola N.E. (2018). BET inhibition overcomes receptor tyrosine kinase-mediated cetuximab resistance in HNSCC. Cancer research, 78(15), 4331-4343.
+ Open protocol
+ Expand
3

Prostate Cancer Cell Line Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human PC3, DU145 and LNCaP cell lines were obtained from ATCC (Manassas, VA) and maintained in DMEM High Glucose (PC3 and DU145) or RPMI-1640 (LNCaP) (HyClone) with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 100 μg/ml streptomycin in 5% CO2 humidified atmosphere at 37°C. Primary antibodies against cleaved caspase-3, cleaved caspase-9, N-Cadherin, p-GSK3 Ser9/21, β-catenin and Snail were purchased from Cell Signaling (Boston, MA), and anti-β-actin was from Sigma (St Louis, MO). Anti-mouse and anti-rabbit HRP conjugated secondary antibodies were obtained from BioRad (Hercules, CA). Human dermal microvascular endothelial cells (HMEC) were obtained from ATCC. Cells were maintained in Endothelial Cell Basal Medium-2 (Lonza) with Microvascular Endothelial Cell Growth Medium-2 Bullet Kit (Lonza). All cultures were maintained in a humidified 5% CO2 incubator at 37°C, and routinely passaged when 80–90% confluent.
Gao F., Al-Azayzih A, & Somanath P.R. (2015). Discrete functions of GSK3α and GSK3β isoforms in prostate tumor growth and micrometastasis. Oncotarget, 6(8), 5947-5962.
+ Open protocol
+ Expand
4

Prostate Cancer Cell Line Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Metastatic (Androgen independent) prostate cancer cell lines (PC3 and DU145) were obtained from ATCC (Manassas, VA) and maintained in DMEM-High Glucose (Hyclone, Logan, UT) with 10% FBS, 100 units/ml penicillin and 100 μg/ml streptomycin in 5% CO2 humidified atmosphere at 37 °C. Primary antibodies against p-Pak1/2, total Pak1, p-Smad2/3, total Smad2/3, E-cadherin, N-cadherin, Vimentin, Keratin8/18, Snail, Slug, cleaved caspases 9 and 3, TGFβ1, TRAF6 and p-P38-MAPK were purchased from Cell Signaling (Boston, MA). Primary antibody against Rac1 and β-actin were purchased from Sigma-Aldrich (St Louis, MO). Anti-mouse and anti-rabbit HRP conjugated secondary antibodies were purchased from Bio-Rad (Hercules, CA). Selective Pak1 inhibitor (IPA 3) was purchased from Tocris bioscience (Minneapolis, MN). SiPak1 and control siRNAs were purchased from Cell Signaling (Boston, MA). Rh-TGFβ1 was purchased from R&D systems (Minneapolis, MN). SiTRAF6, SiSmad2 and AlexaFluor-labelled Phalloidin were purchased from Life technologies (Carlsbad, CA).
Al-Azayzih A., Gao F, & Somanath P.R. (2015). P21 Activated Kinase-1 Mediates Transforming Growth Factor β1-Induced Prostate Cancer Cell Epithelial to Mesenchymal Transition. Biochimica et biophysica acta, 1853(5), 1229-1239.
+ Open protocol
+ Expand
5

Quantitative Analysis of ACE2 Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell lysates were prepared using 1X RIPA lysis buffer (Millipore, Temecula, CA) supplemented with protease and phosphatase inhibitor tablets (Roche Applied Science, Indianapolis, IN). Protein concentration was measured by the DC protein assay (Bio-Rad Laboratories, Hercules, CA) and approximately 40–50 μg of cell lysates in Laemmli buffer were used. Densitometry was performed using NIH ImageJ software. Primary antibodies against ACE2 (Cat No. MABN59, Millipore Sigma, Burlington, MA), β-actin (Cat No. A2228, Millipore Sigma), and β-tubulin (Cat No. 2118, Cell Signaling, Danvers, MA), pSer473 Akt (Cat No. 9271, Cell Signaling) and Akt (Cat No. 9272, Cell Signaling) were used in the study. Anti-mouse and anti-rabbit HRP conjugated secondary antibodies were obtained from Bio-Rad, Hercules, CA.
Adil M.S., Verma A., Rudraraju M., Narayanan S.P, & Somanath P.R. (2021). Akt‐independent effects of triciribine on ACE2 expression in human lung epithelial cells: Potential benefits in restricting SARS‐CoV2 infection. Journal of Cellular Physiology, 236(9), 6597-6606.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!