Fluo-4, AM, cell permeant (Molecular Probes®) 10 × 50 µg (Life Technologies; catalogue number: F-14201). Calcium Orange (Thermo Fisher; catalogue number: C3015). DRAQ5™ Fluorescent Probe Solution (5 mM; Thermo Fisher; catalogue number: 62251). LysoTracker™ Red DND-99—Special Packaging (Thermo Fisher; catalogue number: L7528). ER-Tracker™ Blue-White DPX, for live-cell imaging (Thermo Fisher; catalogue number: E12353). MitoTracker™ Green FM (Invitrogen; catalogue number: M7514). Thapsigargin (ABCAM; catalogue number: ab120286); Maurocalcine (ABCAM; catalogue number: ab141860). Angiotensin II (ABCAM; cataologue number: ab120183); Tetracaine (Sigma-Aldrich catalogue number: T7383).
Angiotensin 2
Manufactured by Abcam
Angiotensin II is a laboratory reagent that functions as a vasoconstrictor, promoting the constriction of blood vessels. It is a key component of the renin-angiotensin system, which plays a role in the regulation of blood pressure and fluid balance. Angiotensin II is commonly used in research applications to study cardiovascular and renal physiology.
Automatically generated - may contain errors
Lab products found in correlation
Tetracaine, by Merck Group (1 mentions)
Calcium orange, by Thermo Fisher Scientific (1 mentions)
Mitotracker green fm, by Thermo Fisher Scientific (1 mentions)
Thapsigargin, by Abcam (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Its premix, by Corning (1 mentions)
Forskolin, by Abcam (1 mentions)
Pd123319, by Bio-Techne (1 mentions)
Potassium chloride (kcl), by Bio-Techne (1 mentions)
3 protocols using angiotensin 2
1
Multicolor Cellular Imaging Techniques
2
Investigating H295R Steroidogenic Capacity
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The H295R cell line was grown in DMEM-F12 medium (GIBCO) with Nu serum and ITS-Premix (Corning). Antibodies against SVIP (Sigma-Aldrich, HPA039807), CYP17A1 (Santa Cruz, sc-374244), CYP11A1(CST, 14217), CYP11B1 (ABCAM, Ab-229884), HSD3β2 (ABCAM, Ab-75710), HMGR (ABCAM, Ab-174830), StAR (ABCAM, Ab-58013), LDLR (ABCAM, Ab-52818), caspase 3 (CST, 9665), cleaved-caspase 3 (CST, 9664), PARP-1 (CST, 9542), eIF2α (CST, 9722), p-eIF2α (CST, 9721), anti-LC3 (CST, 12741), anti-p62 (CST, 5114), actin (Sigma-Aldrich, A5316), and GAPDH (CST, 5174) were used for protein quantity determination by immunoblotting. The Organelle Localization IF Antibody Sampler Kit (CST, 8653) was used in immunofluorescence co-localization experiments. HRP-conjugated anti-mouse or anti-rabbit IgG was purchased from Pierce.
Cycloheximide (66-81-9) was purchased from Calbiochem and 8Br-cAMP (Ab-141448), forskolin (Ab-120058), and angiotensin II (Ab-120183) were purchased from ABCAM.
Cycloheximide (66-81-9) was purchased from Calbiochem and 8Br-cAMP (Ab-141448), forskolin (Ab-120058), and angiotensin II (Ab-120183) were purchased from ABCAM.
3
Vasodilator Compounds in Saline
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