The following compounds were used in our experiments: fisetin (F; Cayman, Ann Arbor, MI, USA), peimine (P; Sigma‒Aldrich, St Louis, USA), artemisinin (Ar; Sigma‒Aldrich, St. Louis, MI, USA), astaxanthin (A; Sigma‒Aldrich, St Louis, USA), bardoxolone methyl (BM, Sigma‒Aldrich, St. Louis, MI, USA), 740 Y-P (7; Med Chem Express, Monmouth Junction, NJ, USA), morphine hydrochloride (M; Fagron, Krakow, Poland), buprenorphine (B; Polfa S.A., Warsaw, Poland) and oxycodone hydrochloride (O; Molteni Farmaceutici, Scandicci, Italy). All of these substances were administered via intrathecal injection (i.t.), a standard procedure in our laboratory [109 (link),111 (link)]. It is performed by using a Hamilton syringe with a thin needle in accordance with instructions described previously [112 (link)]. The injections were performed in the lumbar segment of the spinal cord (between the L5 and L6 vertebrae) with a volume of 5 µL, and the tail reflex was an indicator of correct administration.
Bardoxolone methyl bm
Manufactured by Merck Group
Sourced in United States
Bardoxolone methyl (BM) is a synthetic triterpenoid compound developed by Reata Pharmaceuticals and licensed to Kyowa Kirin for clinical development and commercialization. BM is a nuclear factor erythroid 2-related factor 2 (Nrf2) activator that has been studied for its potential therapeutic effects in various disease conditions.
Automatically generated - may contain errors
Lab products found in correlation
Astaxanthin, by Merck Group (1 mentions)
Artemisinin, by Merck Group (1 mentions)
Morphine hydrochloride, by Fagron (1 mentions)
Peimine, by Merck Group (1 mentions)
Rpmi 2650, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Sh nc, by RiboBio (1 mentions)
2 protocols using bardoxolone methyl bm
1
Intrathecal Administration of Bioactive Compounds
2
Nasal Epithelial Cell Transfection
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Human NPC cell lines CNE1, CNE2 and HNE1 were obtained from the Cellbank of Chinese Academy of Sciences (Shanghai, China), maintained at 37°C with 5% CO2 in Dulbecco's Modified Eagle Medium (Invitrogen, California, USA) and supplemented with 10% fetal bovine serum (FBS; Gibco, USA). The human nasal epithelial cell line RPMI2650 was obtained from ATCC (USA) and cultured in minimum essential medium (Gibco, USA) supplemented with 10% FBS at 37°C with 5% CO2.
The CNE1 and HNE1 cells were transfected by pcDNA3.0 plasmid (NC), pcDNA3.0-LAMB3 (LAMB3), shNC, shLAMB-1# or shLAMB-2# (Ribobio, China) following the manufacturer’s standard protocol.
For co-transfection, the CNE1 and HNE1 cells were co-transfected with shLAMB-2# or shNC and shFOXO3 following the manufacturer’s standard protocol.
To activate NRF2 signaling pathway, the cells were pretreated with 10 nM bardoxolone methyl (BM; Sigma-Aldrich, USA).
The CNE1 and HNE1 cells were transfected by pcDNA3.0 plasmid (NC), pcDNA3.0-LAMB3 (LAMB3), shNC, shLAMB-1# or shLAMB-2# (Ribobio, China) following the manufacturer’s standard protocol.
For co-transfection, the CNE1 and HNE1 cells were co-transfected with shLAMB-2# or shNC and shFOXO3 following the manufacturer’s standard protocol.
To activate NRF2 signaling pathway, the cells were pretreated with 10 nM bardoxolone methyl (BM; Sigma-Aldrich, USA).
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