Human pulmonary artery endothelial cells (HPAEC) were obtained from Lonza (East Rutherford, NJ) and used for experiments at passages 5–8. ANP and TRAP6 were purchased from Ana Spec (San Jose, CA). Reagents for immunofluorescence were purchased from Molecular Probes (Eugene, OR). HRP-linked anti-mouse and -rabbit IgG antibodies were obtained from Cell Signaling Inc. (Beverly, MA). Antibodies to phospho- myosin phosphatase targeting subunit1 (MYPT), GEF-H1, diphospho-myosin light chain (MLC), phospho-stathmin, and IκBα were from Cell Signaling Inc (Beverly, MA); stathmin and End-Binding protein-1 (EB1) were from BD Transduction Laboratories (San Diego, CA); ICAM-1 and VCAM-1 were from Santa Cruz Biotechnology (Santa Cruz, CA). Peptidoglycan G (PepG) polymer covers cell surface of S. aureus Gram positive bacteria. PepG of 99% purity isolated from S. aureus, cat-77140, was obtained from Fluka (Buchs, Switzerland). Unless specified, other biochemical reagents including β-Tubulin and acetylated tubulin antibodies were obtained from Sigma (St. Louis, MO).
End binding protein 1 eb1
Manufactured by BD
End-Binding protein-1 (EB1) is a microtubule-associated protein that plays a crucial role in the dynamics and organization of microtubules. It functions as a regulator of microtubule polymerization and is involved in various cellular processes, such as cell division and intracellular trafficking.
Automatically generated - may contain errors
Lab products found in correlation
Stathmin, by BD (2 mentions)
Acetylated tubulin, by Merck Group (1 mentions)
Trap 6, by AnaSpec (1 mentions)
Vcam 1, by Santa Cruz Biotechnology (1 mentions)
β tubulin, by Merck Group (1 mentions)
Icam 1, by Santa Cruz Biotechnology (1 mentions)
Hpaec, by Lonza (1 mentions)
Phospho mlc, by Cell Signaling Technology (1 mentions)
Phospho mypt, by Cell Signaling Technology (1 mentions)
Gef h1, by Cell Signaling Technology (1 mentions)
Phospho y421 cortactin, by Cell Signaling Technology (1 mentions)
Human hgf, by R&D Systems (1 mentions)
Reagents for immunofluorescence, by Thermo Fisher Scientific (1 mentions)
2 protocols using end binding protein 1 eb1
1
Endothelial Cell Signaling Protocol
2
Cultured Human Endothelial Cells Analysis
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Human pulmonary artery endothelial cells (HPAEC) were obtained from Lonza (East Rutherford, NJ). Human HGF was from R&D (Minneapolis, MN). c-Met kinase inhibitor, N-(3-Fluoro-4-(7-methoxy-4-quinolinyl)phenyl)-1-(2-hydroxy-2-methylpropyl)-5-methyl-3-oxo-phenyl-2,3-dihydro-1H-pyrazole carboxamide, was from Millipore (Billerica, MA). Reagents for immunofluorescence were purchased from Molecular Probes (Eugene, OR). Antibodies to phospho-MYPT, GEF-H1, PAK1, phospho-MLC, phospho-Y421 cortactin were from Cell Signaling (Beverly, MA); stathmin, and End-Binding protein-1 (EB1) were from BD Transduction Laboratories (San Diego, CA); Rac1, RhoA, His-tag were from Santa Cruz Biotechnology (Santa Cruz, CA). Stathmin phospho-S63 specific antibody, cat. #76583, was from Abcam (Cambridge, MA). Unless otherwise specified, all biochemical reagents were obtained from Sigma (St. Louis, MO).
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